免疫化学

The immunocytochemistry position of cell cycle proteins in hepatocellular carcinoma cytogeny HCC????

杨连君，王文亮，司晓辉。细胞周期相关蛋白在肝癌细胞系HCC？？？？9204表达的免疫细胞化学定位［J］。

Under fluorescence microscope, cytolymph could emit red fluorescence with Rhodamine, and cellular nucleus could emit blue fluorescence with DAPI, accompanying myofilament structure.

免疫细胞化学检测：荧光显微镜下，罗丹明染色细胞浆呈红色荧光，并可见肌丝结构；DAPI染色细胞核呈蓝色荧光。

Methods Immunohistochemical streptavidin-peroxidase method was used to detect the expression of MMP-2, MMP-9, TIMP-1, TIMP-2 in 20 cases of normal human cytotrophoblast cells,15 cases of partial hydatidiform moles, 55 cases of complete hydatidiform moles, 15 cases of invasive moles and 8 cases of choriocarcinoma.

应用链霉菌抗生物素蛋白-过氧化物酶免疫组织化学方法对20例正常早孕(2周)绒毛组织、15例部分性葡萄胎、55例完全性葡萄胎、15例侵蚀性葡萄胎、8例绒毛膜癌组织中MMP-2、MMP-9、TIMP-1、TIMP-2进行检测。

Mehtods Thirty male SpragueDawley rats were randomly divided into acute stress,chronic stress and control group(each n=10),acute and chronic stress models were made with forced swimming and empty bottle stimulation separately,and frequency of getting into central grille, detention time , outer grille numbers,modifying numbers and dejecture grain number were observed with openfield experimental box;expressions of neuropeptide Y and adenylcyclase in prefrontal lobe cortex, hippocampus CA3,striatum of the groups were observed using the immunohistochemistry method and correlation analyses conducted.

将30只雄性SD大鼠随机分为急性应激组、慢性应激组和正常对照组，每组10只，用强迫游泳和空瓶刺激分别制作急性和慢性应激模型，采用开野实验箱观察各组大鼠进入中央格次数、停留时间、外周格子数、修饰次数、粪便粒数；采用免疫组织化学方法检测各组大鼠神经肽Y、腺苷酸环化酶在前额叶皮质，海马CA3区，纹状体的表达水平，并进行相关分析。

Mehtods Thirty male Sprague??Dawley rats were randomly divided into acute stress,chronic stress and control group(each n=10),acute and chronic stress models were made with forced swimming and empty bottle stimulation separately,and frequency of getting into central grille, detention time , outer grille numbers,modifying numbers and dejecture grain number were observed with open??field experimental box;expressions of neuropeptide Y and adenylcyclase in prefrontal lobe cortex, hippocampus CA3,striatum of the groups were observed using the immunohistochemistry method and correlation analyses conducted.

将30只雄性SD大鼠随机分为急性应激组、慢性应激组和正常对照组，每组10只，用强迫游泳和空瓶刺激分别制作急性和慢性应激模型，采用开野实验箱观察各组大鼠进入中央格次数、停留时间、外周格子数、修饰次数、粪便粒数；采用免疫组织化学方法检测各组大鼠神经肽Y、腺苷酸环化酶在前额叶皮质，海马CA3区，纹状体的表达水平，并进行相关分析。

They were misdiagnosed as tuberculosis (6 cases), leukemia(4 cases), chronic tonsillitis(2 cases), lymphadenitis(3 cases) and intussusception,myelitis,didymitis,chronic rhinitis,histocytosis,skin allergy.

恶性淋巴瘤易发生临床误诊，重视病变部位的活检，进行常规病理检查同时进行免疫组织化学检查是减少恶性淋巴瘤误诊的重要措施。

Methods The NGF, BDNF, and NT3 genes of rats were cloned, the eukaryote expression vectors were established, the three kind of recombinant vectors were used to transfect astrocytes, the positive cloned cells were cultured dilatedly after G418 sifting; using supernates of culture liquid of astrocytes modified by gene to culture PC12 or TrkB-PC12, the expression and its level of gene target cells aimed genes were measured by Western blotting or immunohistochemical method.

克隆大鼠NGF、BDNF和NT3基因，构建真核表达载体；3种重组载体分别转染星形胶质细胞，G418筛选后获得阳性克隆细胞进行扩大培养；取基因修饰星形胶质细胞培养液上清培养PC12细胞或TrkB-PC12细胞；用Western blotting杂交或免疫组织化学方法检测基因靶细胞目的基因的表达及其水平。

Methods HA and TGFβ1 were measured concurrently in synovial fluid samples from 23 patients with mild OA, 28 patients with severe OA and 6 patients with disciform meniscus. The determinations of positive rate of cellsin genual synovium with HA and TGFβ1 were detected by using immunohistochemical techonology.

提取23例OA病变轻微患者，28例病变严重患者，6例对照者的滑膜组织及关节液，采用免疫组织化学方法检测膝关节滑膜组织中HA、TGFβ1的阳性细胞率及通过夹心ELISA法测定膝关节滑液中HA、TGFβ1含量。

Methods IL-1and MMP-13were measured concurrently in synovial fluid samples from23patients with mild OA,28patients with severe OA and6patients with disciform meniscus.The determinations of positive rate of cells in genual synovium with IL-1and MMP-13were carried out using immunohistochemical techonology.

提取23例OA病变轻微患者、28例OA病变严重患者、6例对照者的滑膜组织及关节液，采用免疫组织化学方法检测膝关节滑膜组织中IL-1、MMP-13的阳性细胞率及通过夹心ELISA法测定膝关节滑液中IL-1、MMP-13含量。

Objective To study the relationship between IL-1,MMP-13and pathogenesis of knee osˉteoarthrosis.Methods IL-1and MMP-13were measured concurrently in synovial fluid samples from23patients with mild OA,28patients with severe OA and6patients with disciform meniscus.The determinations of positive rate of cells in genual synovium with IL-1and MMP-13were carried out using immunohistochemical techonology.

目的 探讨白细胞介素-1（IL-1）、基质金属蛋白酶-13（MMP-13）在膝关节骨性关节炎发病中的作用方法提取23例OA病变轻微患者、28例OA病变严重患者、6例对照者的滑膜组织及关节液，采用免疫组织化学方法检测膝关节滑膜组织中IL-1、MMP-13的阳性细胞率及通过夹心ELISA法测定膝关节滑液中IL-1、MMP-13含量。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。