免疫化学

Then the cell proliferation activity was detected using MTT colorimetrics, the cell cycle and apoptosis after LPS stimulation using flow cytometry, and expressions of caspase3, Bcl2, Bax using immunocytochemical staining.

mg/L的LPS作用于细胞，采用MTT染色法检测LPS对细胞增殖的影响；采用流式细胞术检测细胞周期及凋亡；采用免疫组织化学染色技术检测细胞的caspase3、Bcl2、Bax表达变化。

Objective To detect the effects of ethology, the neuron and glia of dorsal commissural nucleus in rats with irritable bowel syndrome induced by distented the colon, and provide theory evidence for the pathogeny of IBS.

目的 采用行为学和免疫荧光化学的方法，观察直肠扩张刺激对IBS大鼠痛行为学以及大鼠骶髓后连合核(dorsal commissural nucleus， DCN)中神经元和胶质细胞的影响，为探讨IBS发生机制提供理论依据。

In this paper, modern isolation and analysis methods were used to study on the extraction, purification、characteristics and bioactivities of Coprinus comatus polysaccharides, systematically. The research offers some scientific basis for comprehensive utilization of Coprinus comatus.

本论文以毛头鬼伞子实体为原料，利用现代分离分析手段，对毛头鬼伞粗多糖的提取纯化工艺、理化性质、化学结构以及毛头鬼伞多糖的体外免疫活性进行了较系统的研究。

Methods: 30 adul male Sprague-Dawley rats were divided randomly into model group and control group. The rats were injected into lateral ventricle with coriaria lactone (50g/kg). The sections of the brains were processed by immunochemistry with antibodies against SYN and GFAP.

成年健康雄性SD大鼠30只，随机分为模型组及对照组，采用马桑内酯(50g/kg)侧脑室注射法建立急性癫痫动物模型，应用免疫组织化学方法观察癫痫持续状态下SYN和GFAP的表达。

The model mice were then treated by giving GDNF into the striatum. After a seven-day survival period, the model mice were sacrificed to get the segment of substantial nigra fixed, embedded and coronally sectioned continuously. The microsections were processed by immunohistochemistry using anti -TH and anti-CB antibodies to label DA neurons and CB-containing neurons respectively, which were counted under microscope and analyzed statistically.

利用1-甲基－4-苯基1，2，3，6-四氢吡啶(1-methyl-4-phenyl-1，2，3，6-tetrahydropyridine，MPTP)制备成年小鼠PD模型，通过单侧纹状体定位注射GDNF，取中脑黑质节段，做连续冠状石蜡切片，结合免疫组织化学染色方法，观察各组酪氨酸羟化酶(tyrosine hydroxylase，TH)、钙结合蛋白(calbindin D28k，CB)的表达，光镜观察并细胞计数，统计学分析。

Methods: rat costochondral growth plate chondrocytes were isolated and cultured. growth kinetics of rgcs during serial passage was analyzed, and α sma, collagen typeⅱ and gag expressions of primary to 5th passage rgcs were detected by immunocytochemistry, western blot and alcian blue staining, respectively.

分离、培养大鼠肋生长板软骨细胞，对原代至第5代rgcs进行细胞生长动力学分析，阿尔新兰染色、免疫细胞化学法和蛋白质印迹分别检测蛋白多糖，α sma和ⅱ型胶原的表达。

In ACC, NGF,TrkA and p75 mainly existed in cells of duct-like, cribriform and mass-like structures.According to the optical density scanning of immunohistochemistric stain of NGF and TrkA in salivary gland and tumor tissues and quantification analysis, it was shown that the average optical density of NGF and TrkA increased in order of salivary gland tissues around neoplasm, mucoepidermiod carcinoma, and adenoid cystic carcinoma.

2叫 吉林大学硕士学位论文通过对NGF和TrkA在涎腺组织和涎腺肿瘤中的免疫组织化学染色结果进行光密度扫描，对所测平均光密度定量分析，结果显示：NGF和TrkA平均光密度值按照肿瘤周围涎腺组织、豁液表皮样癌和腺样囊性癌依次升高，统计学分析在各组间均有极显著差异(P.01)，表明NGF和TrkA通过自分泌机制调控涎腺腺样囊性癌和豁液表皮样癌的生长与增殖，通过旁分泌机制参与涎腺腺样囊性癌向神经周围的浸润。

Adenocarcinoma had a solid trabecular, papillary, cribriform or diffuse pattern. The cribriform-morular and papillary cells were positive for CEA and EMA, but focally positive for AFP by immumohistochemical analysis.

腺癌组织学表现为实体巢状或弥散分布，以及肿瘤性的乳头状上皮、筛状结构，免疫组织化学染色CEA、EMA阳性，而AFP灶性阳性。

The mice were killed 3, 7 and 14 days after operation (8 mice per group at a time), the tissue of cystiform was harvested to receive gross, histology and immunohistochemistry observation, as well as RT-PCR and Western blot detection.

术后观察动物一般情况，于给药后3、7 及14 d，每组取8 只小鼠背部囊壁组织行大体、组织学观察及免疫组织化学、RT-PCR、Western blot 检测。

The mechanism of Si Junzi Tang and panax sapogenol were studied on the effect of gastrointestinal cells protection by applying advanced technology of histochemistry, Holt′s cytodieresis mircocirculation, radio-immunity and subcellular ultrastructure.

进而应用酶组织化学、Holt细胞分裂测定、微循环测定、放射免疫测定、亚细胞超微结构观察等先进技术，研究四君子汤、人参皂甙抗胃肠细胞损伤、保护胃肠细胞的作用机理。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。