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免疫化学

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Occurrence,migration,tissue localization and quantity changes of CD3+,CD4+,and CD8+ T lymphocyte subpopulations in chicken caecal tonsil was examined immunohistochemically by using the CD3,CD4 and CD8 monoclonal antibodies.

通过免疫组织化学方法,应用CD3、CD4和CD8单克隆抗体研究了CD3+ T淋巴细胞及CD4+和CD8+T淋巴细胞亚群在鸡盲肠扁桃体中的出现、迁移、组织定位分布及数量变化规律等一系列发育过程。

Co.,Ltd.,Tianjin 300380,China;4.Beijing Zhaoyan New Drug Research Center Co.,Ltd.,Beijing 100176,ChinaAbstract: The occurrence,migration,tissue localization and quantity changes of IgM+,IgG+ and IgA+ B lymphocytes in chicken caecal tonsil were examined using the IgM,IgG and IgA monoclonal antibodies by immunohistochemical method.

通过免疫组织化学方法,应用IgM、IgG和IgA单克隆抗体研究了IgM+、IgG+和IgA+ B淋巴细胞在鸡盲肠扁桃体中的出现、迁移、组织定位分布以及数量变化规律等一系列发育过程。

PTTG and bFGF were examined in 41 cases of primary gallbladder cancer and 20 cases of chronic calculous cholecystitis tissues by immunohistochemical assay.

应用免疫组织化学,检测 4 1例原发性胆囊癌及 2 0例慢性结石性胆囊炎中PTTG和bFGF的表达水平。

Using CP (Colophony-Paraffin) embedding tissue section technique and immunocytochemical method (Streptavidin-Peroxidase, SP), we have investigated the distribution of GABA and 5-HT immunoreactive neurons in the brain of female and male ants, Camponotus Japonicus. Special attention has been paid to the comparative research on the differences between female and male ants. We also try to give possible interpretations leading to these differences in the behavioral aspects.Here we show that in the brain of female and male C.

本实验采用树脂石蜡组织包埋切片技术(国家发明专利ZL98125709.7)和链霉菌抗生物素蛋白-过氧化物酶(Streptavidin-Peroxidase,SP)免疫组织化学方法,对日本弓背蚁雄蚁和雌蚁成虫脑中的GABA和5-HT能神经元的分布进行了详细的研究,比较了雄蚁和雌蚁的异同,并对可能造成这些差异的行为学联系进行了初步的探讨。

Using Colophony-Paraffin embedding tissue section technique and immunohistochemical streptalidin-peroxidase method, we have investigated the distribution of 5-HT neurons in the brain of Camponotus japonicus, compared the immunoreactivity with GABA, and primarily discussed the character of 5-HT distribution.

本实验采用树脂石蜡组织包埋切片技术和链霉菌抗生物素蛋白-过氧化物酶免疫组织化学方法,研究了5-HT能神经元在日本弓背蚁大工蚁脑中的分布情况,并与GABA的分布进行了比较,初步探讨了5-HT在各个脑区的分布规律和特点。

Methods The animal model of optic nerve injury at the canalicular segment were produced in healthy adult rabbits.

建立家兔视神经损伤的动物模型,利用免疫组织化学方法观察管内段视神经损伤后GAP-43的表达。

Methods RGD peptide were through direct covalent linkage attached on micro-channel surface with 1-ethyl-3- 3-dimethylaminopropyl carbodiimide hydrochloride and N-hydroxysuccinimide to A549 cells.

方法采用化学共价连接的方法固定RGD多肽,建立由流体控制系统、免疫反应系统构成的细胞识别芯片系统,用于细胞的筛选/捕获;选用不同密度的细胞进行进样试验,考察不同细胞密度对通道内细胞数量的影响,并讨论不同的孵育时间对细胞捕获的影响。

Methods RGD peptide were through direct covalent linkage attached on micro-channel surface with 1-ethyl-3- 1 3-dimethylaminopropyl carbodiimide hydrochloride and N- hydroxysuccinimide to A549 cells.

采用化学共价连接的方法固定RGD多肽,建立南流体控制系统、免疫反应系统构成的细胞识别芯片系统,用于细胞的筛选/捕获;选用不同密度的细胞进行进样试验,考察不同细胞密度对通道内细胞数量的影响,并讨论不同的孵育时间对细胞捕获的影响。

decrease or absence expression of pten and fhit protein play an important role in the genesis and development of gastric carcinoma; the two proteins can be used to judge carcinomatous biological behaviors and prognosis.

本研究采用免疫组织化学方法检测56例胃腺癌组织及10例正常组织中pten和fhit基因蛋白的表达情况,探讨其在胃腺癌发生、发展中的作用。

Methods: The expression of GHR were detected by immunohistochemistry technique in cancer specimens and paired normal esophageal tissue from 50 patients with esophageal or cardial cancer.

应用免疫组织化学的方法测定50例食管贲门癌标本及其附近正常食管粘膜中GHR表达情况,分析食管贲门癌组织和正常食管粘膜中GHR表达及其与临床、病理因素的关系。

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