免疫化学

Cell cycle and surface antigen were detected with flow cytometry. Adipogenic differentiation and osteogenic differentiationwere tested by immunohistochemistry.

流式细胞仪测定细胞周期及细胞表型，免疫组织化学染色检测其体外诱导成脂肪和成骨分化的能力。

Corpus cavernosum;Aging;Collagen;Tunica albuginea;Erectile dysfunction;Immunohistochemistry;Penis

中文关键字阴茎海绵体；老化；胶原蛋白；阴茎白膜；勃起功能障碍；免疫组织化学；阴茎

Titanium plate first was cleaned by glow Discharge using argon plasma to eliminate surface contaminants and to produce a consistent and reproducible titanium oxide surface layer. Then an intermediary allylamine deposition was covalently linked to the oxide layer by glow discharge, followed by the covalent binding of albumin to the free terminal NH2 groups using glutaraldehyde as a coupling agent. Surface morphologies were observed by scanning electron microscopy and atomic force microscopy.

样本以扫描式电子显微镜及原子力显微镜﹙AFM﹚观察表面形态变化，以能量分散光谱仪进行表面元素变化之定性分析，使用X光光电子能谱仪检测表面化学键结与元素变化作定性分析与半定量分析，并以胶样金免疫标示法配合使用场发射枪扫描式电子显微镜(Field Emission Scanning Electron Microscope， FEG SEM)，观察钛金属表面白蛋白连接情形作定性分析与定量分析。

Function of 1 bowel protective screen and protective screen of its characteristic bowel include protective screen of alvine mucous membrane, chemical protective screen, biology protective screen and immune protective screen, its are main the function is to resist the bacterium inside alvine antrum and toxin enter system inside.

本文现就化疗药物对肠屏障功能的损伤机制及其防治进展作一综述。1肠屏障功能及其特征肠屏障包括肠黏膜屏障、化学屏障、生物屏障和免疫屏障，其主要功能是抵御肠腔内细菌及毒素进入体内。

KEY WORDS myofibroblastoma; amianthoid fiber; tumor pathology; immunohistochemistry

关键词，肌纤维母细胞瘤；石棉样纤维；肿瘤病理学；免疫组织化学

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

Litter size, sex ratio at birth, and anogenital distance in offspring of both sexes were examined, as well as vaginal opening in female offspring.

采用逆转录聚合酶链反应技术和免疫组织化学染色技术检测各组胎盘ERα、ERβ受体的表达。

Methods:The expression intensity and distribution of PCNA were examined with immunohistochemistry method in 15 cases of recurrent oral aphthous and normal oral mucosa.

用免疫组织化学技术检测 PCNA在 RAU中的表达变化规律。

Methods:The expression intensity and distribution of PCNA were examined with immunohisto chemistry method in 15 cases of recurrent oral aphthous and normal oral mucosa.

方法用免疫组织化学技术检测PCNA在RAU中的表达变化规律。

On the other hand, LNT did not prolong the survival period when combined with S-1 in Colon-26-bearing athymic mice.

本文证明用S-1与LNT结合体内化学免疫治疗的效果，我们阐述了体内外树状突细胞功能的增进。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。