免疫化学

With a model of reversible middle cerebral artery occlusion in rats, the effects of Losartan on the water content of brain, the contents of Evens blue and the expression of MMP-9 were investigated after 24 hours of reperfusion following two hours of cerebral ischemia.

采用血管内栓线阻断法制备大鼠局灶性脑缺血再灌注损伤模型，于脑缺血2h、再灌注24h后测定脑组织含水量、伊文斯蓝含量，免疫组织化学方法测定MMP9表达。

The staining level of NFκB and TGFβ1 of bulbar conjunctiva endepidermis in DM was higher than that in control group and was positive correlation. CONCLUSIONIn type 2 DM, the value of BUT and Schirmer decrease and the degree of cornea fluorescein staining increase is incident to the xeroma.

本研究采用免疫组织化学方法对2型糖尿病患者结膜上皮细胞中TGFβ1和NFκB的表达情况进行了检测，并比较分析了正常人群和2型糖尿病患者的眼表状态。

We detected fasting plasma glucose by glucose oxidase,fasting insulin by chemiluminescent immunoassay, triglyceride and high density lipoprotein-cholesterol by zymology,tumor necrosis factor-αby enzyme linked immunosorbent assay, the reverse of the product of FPG and FIns is used as the insulin sensitivity index,that is,ISI=1/FPG×FIns.

血糖测定采用葡萄糖氧化酶法；胰岛素测定采用化学发光法；血脂测定采用酶法；肿瘤坏死因子-α测定采用双抗体夹心酶联免疫法；胰岛素敏感指数采用空腹血糖与空腹胰岛素乘积的倒数，即ISI=1/FPG ×FIns。

We detected fasting plasma glucose by glucose oxidase, fasting insulin by chemiluminescent immunoassay, triglyceride and high density lipoprotein-cholesterol by zymology, tumor necrosis factor-αby enzyme linked immunosorbent assay, the reverse of the product of FPG and FIns is used as the insulin sensitivity index, that is, ISI=1/FPG×Fins.

血糖测定采用葡萄糖氧化酶法；胰岛素测定采用化学发光法；血脂（甘油三酯TG、高密度脂蛋白HDL-C）测定采用酶法；肿瘤坏死因子-α测定采用双抗体夹心酶联免疫法；胰岛素敏感指数采用空腹血糖与空腹胰岛素乘积的倒数，即ISI=1/FPG ×FIns。

INTERVENTIONS: In experimental group, the emetic, cisplatin, was injected abdominally 10 mg/kg. In the control, the physiological saline of same dose was injected. Afterwards, the activity changes in rats were observed at room temperature, quiet and light-avoided environment. Six hours later, the brain tissue was collected for frozen continuous sectioning. Immunohistochemical staining method was used to observe the distribution of Fos positive neurons in brainstem and forebrain nuclei and to count positive cell.

干预：实验组腹腔注射催吐剂顺氯氨铂10 mg/kg，对照组注射等剂量的生理盐水后，于室温、安静、避光环境下观察大鼠的行为学变化。6 h后取大鼠脑组织，进行冷冻连续切片，用免疫组织化学染色方法观察大鼠脑干和前脑核团的Fos阳性神经元的分布，并进行阳性细胞记数。

HRP retrograde tracing ; abducens nucleus ; vestibular nucleus ;γ-amino-butyric acid; immunohistochemical staining ; rats

HRP逆行标记；展神经核；前庭神经核；γ-氨基丁酸；免疫组织化学；大鼠

The double labelling technique by combination of horseradish peroxidaseretrograde tracing with immunohistochemical staining for γ-amino-butyric acidwas used to observe the distribution of GABA-positive neurons in the abducens and vestibular nucleus,and its projection to oculomotor nucleus of rats.

用辣根过氧化物酶(horseradish peroxidase，HRP)逆行标记结合γ-氨基丁酸(γ-amino-butyric acid，GABA)的免疫组织化学双标技术观察大鼠的展神经核和前庭神经核内GABA阳性神经元的分布，以及其向动眼神经核的投射。

Methods Tissue samples were collected from 20 patients with condylomata acuminate and 15 normal human controls.

采用EliVisionTMplus免疫组织化学技术分别检测20例尖锐湿疣和15例正常人对照皮肤中TGFβRⅠ、TGFβRⅡ、Smad1/2/3、磷酸化Smad2/3（p-Smad2/3）和Smad4的表达。

METHODS: expression of Pgp and MDR1 mRNA were detected by immunohistochemistry and reverse transcriptionpolymerase chain reaction in the specimens from 25 breast cancer patients with neoadjuvant chemotherapy, 15 breast cancer patients without neoadjuvant chemotherapy and 10 patients with breast adenosis.

采用免疫组织化学及逆转录多聚酶链反应技术，对25例接受术前新辅助化疗的乳腺癌患者、15例未接受术前化疗的乳腺癌患者、10例乳腺腺病患者组织标本内Pgp 和MDR1 mRNA的表达情况进行检测。

METHODS: The expression of Pgp and MDR1 mRNA were detected by immunohistochemistry and reverse transcriptionpolymerase chain reaction in the specimens from 25 breast cancer patients with neoadjuvant chemotherapy, 15 breast cancer patients without neoadjuvant chemotherapy and 10 patients with breast adenosis.

采用免疫组织化学及逆转录多聚酶链反应技术，对25例接受术前新辅助化疗的乳腺癌患者、15例未接受术前化疗的乳腺癌患者、10例乳腺腺病患者组织标本内Pgp 和MDR1 mRNA的表达情况进行检测。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。