免疫化学

Intimal areas were measured using morphometric analysis of perfusion-fixed vein graft specimens, and intimal thickness was calculated using circumferential measurements. The SMC proliferation was studied by the immunohistochemical detection of proliferating cell nuclear antigen. Expression of MMP-2、MMP-9 mRNA in vein grafts and unoperated control Vein grafts was detected by reverse transcription polymerase chain reaction. Substrate gel zymography was used to determine the proteolytic activity.

分别行HE染色、Verhoeff弹性纤维染色观察组织病理变化，计算机病理图象分析系统测量新生内膜厚度及面积，免疫组织化学方法检测静脉壁增殖细胞核抗原表达以观察细胞增殖情况，半定量逆转录-聚合酶链反应检测静脉壁基质金属蛋白酶-2(MMP-2)和基质金属蛋白酶-9(MMP-9)mRNA的表达，明胶酶谱法检测MMP-2、MMP-9活性，比较各组之间的差别。

Methods Twenty-two adolescent SD female rats were selected. All rats were killed by dislocation of cervical vertebra, the eyeballs were in paraffin imbedding and made to a series of sections, using immunohistochemical method; ERα and ERβ distribution were investigated in uvea tissue of rats; and quantitied by Tanaka scores analytical method. The uteri of rats was used as positive control and PBS as negative control.

选择青春期SD雌性大鼠22只，采用颈椎脱臼处死大鼠，取眼球作常规石蜡包埋，连续切片， SP免疫组织化学方法显示ERα、ERβ在葡萄膜组织的表达；采用Tanaka记分法对ERα、ERβ进行定量分析，并设正常大鼠子宫作阳性对照；PBS代替一抗作阴性对照。

And use immunohistochemical methods to observe the vascularization and infiltration.

用免疫组织化学观察血管形成与浸润的关系。

MethodsExpressions of SP and vasoactive intestinal peptide in tongue base tissues of 49 patients with OSAHS were analyzed by immunohistochemical staining.

方法通过免疫组织化学染色对49例OSAHS患者的舌根组织的P物质及血管活性肠肽的表达进行分析。

The morphological changes of VEC in each group were observed by the inverted microscope, and the expression of ICAM-1 in each group was detected by immunocytochemistry.

通过倒置相差显微镜观察和比较，各组内皮细胞在形态上的变化，用免疫细胞化学染色方法检测ICAM-1在各组内皮细胞中的表达。

Methods 34 cases were invesigated under the light microscopic l e vel and were studied by immunohistochemical technics.

报告34例鼻腔圆形细胞恶性肿瘤，观察其病理组织学表现，用免疫组织化学方法标记肿瘤。

The method is taken not pregnant estrum and pregnant 1 D, 2 D, 3 D, 4 D in the morning 4, D afternoon 4, the small rat uterus of D midnight, 5 D, 6 D, 7 D, 8 D makes section, with immune organization chemical coloring and image analyse a technology to be to STAT3 inside uterus of early pregnant small rat velar expression undertakes detecting.

方法取未孕动情期及孕1 d、2 d、3 d、4 d上午4、d下午4、d午夜、5 d、6 d、7 d、8 d的小鼠子宫做切片，用免疫组织化学染色和图像分析技术对STAT3在早孕小鼠子宫内膜的表达进行检测。

The density of AR of HFSC is consistent with the capability of differentiation of those cells. 3. Cells induced in vitro expressed EMA and Lipid droplets were observed by rathonum red staining, while those as control didn't, which confirmed that androgen can facilitate the differentiation of vibrissa follicle stem cells into sebocytes.

用免疫细胞化学检测到EMA在诱导后的细胞中的表达呈阳性，而在未经诱导处理的细胞中的表达呈阴性；用油红O染色检测到诱导后的细胞中有脂滴的形成，而在未经诱导的细胞中没有脂滴的形成；表明雄激素对毛囊干细胞向皮脂腺细胞定向分化具有促进作用。

Beam walking test was used to evaluate the improvement of motor function at weeks 1,2,3 and 4 after operation. The quantity of growth-associated protein 43(GAP-43) and synaptophysin around ischemia area were examined by immunohistochemical techniques.

术后1周，2周，3周，4周应用横木行走试验评定运动功能改善，免疫组织化学方法检测缺血周围区生长相关蛋白(GAP-43)、突触素SVP）表达的变化。

With a model of reversible middle cerebral artery occlusion in rats, the effects of Losartan on the water content of the brain, the contents of Evens blue and the expression of MMP9 were investigated after 24 hours of reperfusion following two hours of cerebral ischemia.

采用血管内栓线阻断法制备大鼠局灶性脑缺血再灌注损伤模型，于脑缺血2？h、再灌注24？h后测定脑组织含水量、伊文斯蓝含量，免疫组织化学方法测定MMP9表达。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。