免疫化学

METHODS: The expressions of p15, p16 and PCNA proteins in 41 cases of cervical carcinomas and 30 cases of normal cervical tissues were detected by the supersensitive ElivisionTM immunohistochemical method.

应用免疫组织化学ElivisionTM二步法检测41例宫颈癌及30例正常宫颈组织中p16、p15及PCNA的表达情况，并结合病理分级进行分析。

Ovarian carcinoma Oncogene Tumor suppresser gene Immunohistochemistry

卵巢癌；癌基因；抑癌基因；免疫组织化学

Cerebral hemorrhage rat models were established via injection of autologous arterial blood in caudate nucleus. Two days after modeling, 5 μL BrdU-labeled human embryonic NSCs suspension was transplanted at four points surrounding hematoma cavity in the rats. After 1 and 2 weeks, rats were sacrificed. Adjacent sections were doubly stained by BrdU/microtubule-associated protein 2 (MAP-2) and BrdU/glial fibrillary acidic protein.

通过注射自体动脉血到尾状核制作大鼠脑出血模型，出血后2 d将标有5'-溴脱氧尿嘧啶的人胚神经干细胞悬液移植到血肿腔周围的4点，1，2周后处死大鼠，相邻脑组织切片行5'-溴脱氧尿嘧啶/微管相关蛋白2和5'-溴脱氧尿嘧啶/胶质纤维酸性蛋白免疫组织化学双染。

METHODS: Cerebral cortex cells of 8-week aborted human fetus were harvested and cultured in vitro to obtain human embryonic NSCs. Cerebral hemorrhage rat models were established via injection of autologous arterial blood in caudate nucleus. Two days after modeling, 5 μL BrdU-labeled human embryonic NSCs suspension was transplanted at four points surrounding hematoma cavity in the rats.

通过注射自体动脉血到尾状核制作大鼠脑出血模型，出血后2 d将标有5'-溴脱氧尿嘧啶的人胚神经干细胞悬液移植到血肿腔周围的4 点，1，2周后处死大鼠，相邻脑组织切片行5'-溴脱氧尿嘧啶／微管相关蛋白2和5'-溴脱氧尿嘧啶／胶质纤维酸性蛋白免疫组织化学双染。

Methods:Using cell culture and immunohistochemical techniques,the peripheral blood specimens from 33 RA patients were designed to test the human T cell lymphotropic virus type Ⅰ related antigens,reverse transcriptase and spontaneous syncytium formation.

采用细胞培养、免疫组织化学技术等对33例RA患者的外周血样本进行人类嗜T淋巴细胞病毒Ⅰ型相关抗原、逆转录酶活性测定及观察自发性合胞体形成。

Objective To observe the condition of stain of thyroglobulin in thyroid glands and investigate the relationship between the PMI and the condition of degrade of thyroglobulin.

目的 观察不同死亡时间（Postmortem interval，PMI）甲状腺球蛋白的免疫组织化学表达，探讨甲状腺球蛋白的表达特点与PMI之间的关系，为推断PMI的研究提供新的思路和方法，为法医实践中中短期PMI的推断提供科学依据。

Results Same as hyaline cartilage, the calcified cartilage was stained light flavo-green and weak double refraction by the Picrosirius-polarization method. In the immunohistochemistry analysis of collagen Ⅱ protein, the calcified cartilage was stained strong green fluorescence and the collagen protein parallelled in joint surface near the tidemark. The content of collagen Ⅱ protein was (20.16%±0.96%) of tissue dry weight by amino acid analysis, and that of the hydroxyapatite was (65.09%±2.31%) of the inorganic composition in calcified cartilage.

结果 天狼猩红染色见钙化软骨层与透明软骨层一样黄绿色淡染、弱双折光性；Ⅱ型胶原蛋白免疫组织化学分析，钙化软骨层呈强绿色荧光，潮线附近的胶原蛋白呈板层平行分布；氨基酸定量分析钙化软骨层Ⅱ型胶原蛋白含量占组织干质量的（20.16%〖KG-*4〗±0.96%）；X-射线衍射定量分析钙化软骨层中的羟基磷灰石含量占无机成分的（65.09%±2.31%）。

Methods The expression of TM in 40 cases of gastric carcinoma was detected by means of immunohistochemical ABC.

应用免疫组织化学ABC法对40例手术切除胃癌标本进行TM表达的研究。

At day 7, 14 and 21 after induction respectively, Toluidine blue staining and immunocytochemical staining were performed to detect differentiation.

在诱导第7，14，21天分别行甲苯胺蓝染色、免疫细胞化学染色检测其分化结果。

In this study, we make a systematic observation on localazation of mast cells in rabbit by toluidine blue staining method.

本研究采用甲苯胺蓝染色法和免疫组织化学方法，对肥大细胞(Mast cell，MC)在家兔体内的分布定位进行了系统观察。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。