免疫化学

Combining many subjects technological development,such as immunology, chemistry,radiation and shadowgraphy,thoroughly inquires the changeing relation of cells struct

通过结合免疫、化学、放射及影像学等多学科的技术发展，使人们进一步深入了解细胞结构和功能的变化关系，进而阐明材料对细胞的作用机制，是今后细胞培养法评价材料生物相容性的发展方向。

Methods: After stimulation with different concentrations of silicon dioxide, the morphological changes of the above macrophages were observed and the TNF-α expression was detected by immunocytochemistry as well.

用不同浓度的SiO2刺激巨噬细胞后，观察其细胞的形态学改变，并用免疫细胞化学检测其TNF-α的蛋白表达。

Methods The expression of Egr-1 in animal model of silicosis was analyzed by using immunohistochemistry.

用免疫组织化学方法观察大鼠实验性硅沉着病组织中肺巨噬细胞Egr 1表达的动态变化

Concentrations of DA and 5-HT in different regions were measured by high performance liquid chromatography; the expression of SERTmRNA was detected by in situ hybridization; the degeneration of neuron and terminal was demonstrated by Bielschowsky and Glee Marsland silver staining; and the protein expression of GFAP was detected by immunohistochemistry.

2采用高效液相色谱法测定不同脑区神经递质DA和5-HT的含量；采用原位杂交方法检测SERTmRNA；Bielschowsky及Glee Marsland氏改良银浸染法显示神经元胞体、树突和轴突；免疫组织化学方法检测GFAP的表达。

Methods The expression of MMP9 protein was tested by using immunohistochemical SP method in 18 cases of normal cervical epithelium, 20 cases of carcinoma in situs of cervix, and 55 cases of invasive cervical cancer.

采用免疫组织化学SP法检测MMP9蛋白在18例正常宫颈上皮、20例宫颈原位癌、55例宫颈浸润癌中的表达。

Methods: The expression of apoptosis and the positive expression of p53, bcl－ 2 proteins were observed in 31 cases of breast cancer in situs, 20 breast dysplasia and 20 breast hyperplasia by using DNA nick end labeling technique and immunohistochemical staining . Eight normal breast tissues were used as control.

利用DNA缺口末端标记技术和免疫组织化学染色，原位观察31例乳腺癌，20例乳腺不典型增生和20例乳腺单纯性增生中细胞凋亡和p53、bcl－2蛋白的表达，以 8例正常乳腺组织作为对照。

Methods By immunohistochemical method, the expression of bcl-2 and bad gene were observed in 8 cases of normal breast tissues 19 cases of breast hyerplasia, 20 cases of breast atypical, 48 cases of breast cancer in situs ,and test the expression of ER , PR and bcl-2 , bad in the breast cancer, Eight normal breast tissues were used control.

利用免疫组织化学染色SABC法，原位观察19例乳腺单纯性增生，20例乳腺非典型增生，48例乳腺癌组织中bcl-2、bad蛋白的表达以8例正常乳腺组织作对照。

Results ec was cultured and subcultured successfully. vascular endothelial cells show slabstone appearance enchased arrange under phase contrast microscope. the growth appearance was showed under sem. some dispersive high electron density of rod type weible palade body were seen under tem.

结果 培养出血管内皮细胞并传代，倒置显微镜下血管内皮细胞呈"鹅卵石"形态，扫描电镜显示"铺路石"样生长，透射电镜可见内皮细胞特征性w p小体，免疫组织化学鉴定ⅷ因子。

Immunofluorescence was used to analyze the expression of the SMA and identify the cells.

通过免疫细胞化学技术检测血管平滑肌肌动蛋白的表达来鉴定细胞性质。

We also analyzed the processes of secretion of Ustiloxin A in culture medium and in false smut ball in the field by immuno-gold labeling.

通过免疫细胞化学标记，我们分析了稻曲病菌分泌毒素的行为过程，并且分析了田间稻曲球中的毒素的分泌情况。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。