免疫化学

This study that observed the expression and distribution of 4 apoptotic factors, relation apoptotic factor with apoptosis, changes of T and B subgroup, and changes of molecular pathology in 14 cows suffering from enzootic bovine leucosis from several resides was detected by histochemistry, TUNEL technology, immune-fluorescent flow cytometry and so on. The aim of this experiment supplies theoretic basis for preventive and therapy of EBL, and consult dates for T lymphoid cell leucosis of human being .

本研究检查并剖检了14例临床与实验室诊断为EBL的病牛，观察分析了肿瘤发生的情况和特征，采用免疫组织化学ABC法、原位末端标记TUNEL技术和免疫荧光流式细胞检测法等技术，从EBL肿瘤细胞凋亡特性入手，围绕4种凋亡因子和几种免疫相关因子与EBL肿瘤发生的关系及其在诊治方面的应用进行了首次有价值的探索，其目的在于为防治EBL提供科学的理论依据，同时，也将为人类T细胞白血病Ⅰ。

Immunohistochemical method: The CD34 monoclonal antibody was selected as the first antibody, with the immunohistochemical SP procedure, then the pathological slice was displayed by DAB and stained again by hematein. One slice was used as a negative control and reacted with PBS fluid(0.01mmol/L) which was the substitute of anti- CD34.(the kits coming from Boster Biotechnology CO.LTD). The number of the vessels and vascular endothelial cells were displayed by immunohistochemical method.

免疫组化方法：病理组织切片以CD34单克隆抗体作为第一抗体，按照免疫组织化学SP步骤进行，DAB显色，苏木素复染，每组用0.01mmol/l的PBS液替代CD34抗体作阴性对照(试剂购自于武汉博士德公司)，应用免疫组化显示血管，对不同时期的血管生成情况及早期的炎性反映情况进行观察。

In this paper, the ability and mechanism of antibacterial and immune defense ofChinese shrimp was studied using cytological, biochemical, molecular biological andproteomic methods. A sensitive and stable antibacterial and lysozyme assay methodswere selected. The effect of Vibrio anguillarum and laminarin (a kind of beta-1,3-glucan) on the immune ability of haemolymph of Chinese shrimp was analyzed.The changes of total haemocyte counts, antibacterial ability, lysozyme activity andlysosome membrane stability were detected.

本文采用细胞学，生物化学，分子生物学和蛋白质组学等技术，对对虾的抗菌能力和免疫防御机制进行研究，筛选了灵敏稳定的抑菌和溶菌检测方法；并对灭活鳗弧菌和海带多糖(laminarin，一种B-1， 3 葡聚糖)免疫诱导后的中国明对虾血细胞数目，血淋巴的抑菌活力，溶菌能力，溶酶体膜的稳定性进行分析；对两种重要免疫因子溶菌酶和精氨酸激酶进行克隆和表达分析。

The results of immuhistochemistry show: compared with normal retina, more 3-NT-positive cells and iNOS-positive cells appear in inner nucleus layer of diabetic retina; less eNOS-positive cells appear in inner nucleus layer and vascular endoderm of diabetic retina; less nNOS -positive cells appear in inner nucleus layer of diabetic retina; more ET-positive cells, ETRA- positive cells, ETRB- positive cells appear in inner nucleus layer of diabetic retina; moreα- synuclein- positive cells appear in ocular cone and rod layer of diabetic retina. Conclusions 1. RFDD-PCR is an efficient technique for research diseases genomics as a mass screening to complete gene expression with the identifying of candidate gene related to disease.

免疫组织化学结果显示：13-NT和NOS：与正常视网膜相比，8周糖尿病大鼠视网膜中，INL的3-NT和iNOS免疫阳性细胞明显增多，INL和血管内皮层的eNOS阳性细胞明显减少，INL的nNOS阳性细胞也明显减少；2ET及ETR：与正常视网膜相比，8周糖尿病大鼠视网膜中，ET、ETRA、ETRB免疫阳性细胞明显增多，增多的阳性细胞主要集中于INL，而在血管内皮层增多不明显；3α-synuclein：与正常视网膜相比，8周糖尿病大鼠视网膜中，α-synuclein免疫阳性细胞明显增多，增多的阳性细胞主要集中于视网膜视锥视杆层。

Methods: The expression of P57(superscript kip2) was examined in proliferating, involuting human hemangiomas and normal skin tissues by using immunohisto-chemical technique. Immunohistochemical technique for factor Ⅷ-related antigen was used to identify that the cells which expressed P57(superscript kip2) were endothelia.

采用免疫组织化学方法检测人皮肤血管瘤增生期、退化期及正常皮肤组织中P57(上标 kip2)的表达水平，并结合第Ⅷ因子相关抗原的免疫组织化学染色证实表达P57(上标 kip2)的细胞是血管内皮细胞。

Methods Fifteen healthy, female SD rats were employed in the experiment. Sensitized systematically with keyhole limpet hemocyanin, the animals were inoculated with the same antigen through cochlea basal turn into the labyrinth. Adminstrated 5-bromo-2′-deoxyuridine intraperitoneally, the rats were sacrificed and the temporal bones were harvested at 3, 7,14 day after labyrinth vaccination respectively. The frozen sections of the decalcified samples were dealt with H-E staining and immunohistohemical methods to investigate the cellular infiltration, BrdUrd and IgG positive cells in the ES.

选用SD大鼠15只，以钥孔虫戚血蓝蛋白（keyhole limpet hemocyanin，KLH）全身免疫后，经耳蜗底回钻孔以相同抗原进行内耳免疫，然后分别在内耳免疫后3、7和14 d腹腔注射溴脱氧尿嘧啶核苷（bromodeoxyuridine，BrdUrd）后处死动物，取颞骨经组织学处理制作冰冻切片，用免疫组织化学技术观察内淋巴囊的细胞浸润、增殖和IgG细胞的分布状况。

Chemiluminescence Immunoassay is an efficient detection tool combined from chemiluminescent reactions and immunoassay.

化学发光免疫技术是化学发光与免疫测定结合起来的一种高效检测手段。

MAIN OUTCOME MEASURES: Morphology change of spinal cord was detected by light microscope and electronmicroscope, secreted brain-derived neurotrophic factor was measured by immunohistochemistry and PT-PCR, and the combine behavioral score was used to assess the functional recovery of spinal cord. RESULTS: Immunohistochemistry result showed that OECs transplantation could partly reduce the number of apoptosis cells in spinal gray matter, delay cells reduction in spinal white matter, and accelerate the medullary sheath repair and regeneration.

主要观察指标：应用光学显微镜、电子显微镜观察脊髓形态的变化，采用免疫组织化学、PT-PCR 的方法检测脊髓组织脑源性神经营养因子的分泌，以改良的Gale联合行为评分法对脊髓功能进行评定，结果：免疫组织化学检测显示，嗅鞘细胞移植能部分改善大鼠脊髓灰质神经细胞的凋亡程度，延缓白质神经纤维的减少，促进髓鞘的修复与再生。

Each 1 μL site of spinal cord receiving 109 /L OECs at the speed of 1μL/ min. MAIN OUTCOME MEASURES: Morphology change of spinal cord was detected by light microscope and electronmicroscope, secreted brain-derived neurotrophic factor was measured by immunohistochemistry and PT-PCR, and the combine behavioral score was used to assess the functional recovery of spinal cord. RESULTS: Immunohistochemistry result showed that OECs transplantation could partly reduce the number of apoptosis cells in spinal gray matter, delay cells reduction in spinal white matter, and accelerate the medullary sheath repair and regeneration.

主要观察指标：应用光学显微镜、电子显微镜观察脊髓形态的变化，采用免疫组织化学、PT-PCR 的方法检测脊髓组织脑源性神经营养因子的分泌，以改良的Gale联合行为评分法对脊髓功能进行评定，结果：免疫组织化学检测显示，嗅鞘细胞移植能部分改善大鼠脊髓灰质神经细胞的凋亡程度，延缓白质神经纤维的减少，促进髓鞘的修复与再生。

The results showed that (1) COX-2 mRNA and protein existed in the mature male rat testis. COX-2 was localized in spermatocytes by immunohistochemistry.(2) COX-2 also existed in adult man testis. COX-2 protein was positively stained in spermatocytes and Leydig cells.(3) 2 weeks after administration of rofecoxib, the level of testosterone in the whole testis reached its lower values, being only 50% of control values. However, testosterone level recovered during 4 weeks. After such treatment, histologic examination of these testes showed atrophy of the seminiferous tubules and maturation arrest of spermatogenesis.(4) 2 weeks post-EDS, expression of COX-2 decreased significantly (P.005), in comparison with vehicle-treatment control. 4 weeks after treatment, a new generation of fetal like Leydig cells repopulated in the testicular interstitium resulting in COX-2 expression partially recovered. Although the expression of COX-2 mRNA and protein are enhanced at 8th week after using external testosterone, it wasn't significant higher than control group.

实验研究证明：(1)正常成年雄性大鼠睾丸组织中COX-2在mRNA和蛋白质水平均存在表达，免疫组织化学结果显示COX-2定位于曲细精管内的生精细胞；正常成年男性睾丸组织中同样存在COX-2表达，免疫组织化学结果显示COX-2定位于曲细精管内的生精细胞和Leydig细胞；(2)服用特异性COX-2酶抑制剂rofecoxib 2周后，实验组大鼠睾丸组织内睾酮的含量减少，为正常对照组的50％；持续用药4周后睾丸组织内睾酮浓度逐渐恢复至正常水平；COX-2酶活性降低后病理组织切片显示睾丸内曲细精管萎缩，生精紊乱，持续用药4周时影响最明显；(3)注射特异性Leydig细胞杀灭剂EDS 2周后，实验组大鼠睾丸组织内睾酮浓度降至极低水平时，COX-2的蛋白和基因表达水平也显著低于正常空白对照组（P.005）；使用EDS后第4周，睾丸组织中的睾酮浓度逐渐回升，同样组织中COX-2蛋白表达和mRNA表达水平也相应提高接近正常水平；使用EDS后4周开始给予外源性睾酮，6周和8周时COX-2表达水平的绝对值虽有提高，但与正常大鼠空白对照组比较并无显著性差异，说明外源性雄激素刺激睾丸合成COX-2的作用并不明显。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。