免疫化学

Chondrogenesis was assessed with Alcian blue staining, Safranin O/Fast Green staining and collagen Ⅱ immunohistochemistry at 4, 7, and 14 days after initial chondrogenic induction.

在诱导后4，7，14 d应用阿尔新兰染色、蕃红O/固绿染色和Ⅱ型胶原免疫细胞化学评价软骨形成情况。

The rabbit model of osteoarthritis was established according to Hulth's method. The rabbits were sacrificed at the 8th week after administration of Yanghe Decoction for 14 days, and then rabbit tibia articular cartilage was removed. Sections of the cartilage were stained with Safranin O for histological examination. The cartilage histological characteristics were observed according to the method of Mankin. Immunohistochemical staining was performed to investigate the expression of VEGF.

按照Hulth法建立膝骨关节炎模型，术后第5周起至第7周末，阳和汤组予混有阳和汤的颗粒饲料，正常组及模型组均给予普通饲料；术后第8周分别取各组胫骨平台关节软骨，采用番红O染色，进行Mankin评分；免疫组织化学染色测定关节软骨中VEGF表达的阳性指数。

The central part was composed of hypertrophic chondrocytes, for which the number increased with time. The cartilaginous tissue at the time of 2 weeks' cultivation appeared strongly positive staining of collagen type Ⅱ immunohistochemistry and safranine "O", as well as strongly metachromatic to toluidine blue.

其中心为肥大软骨细胞，且随培养时间的延长而增多。2周时，软骨组织甲苯胺蓝染色呈强的红色异染反应，Ⅱ型胶原免疫组织化学染色呈强阳性，番红"O"染色呈强阳性。

RESULTS: HE and safranine O staining both showed no cellular structure in the matrix with only recesses left by the removed cells.

软骨蛋白聚糖免疫组织化学染色阳性，提示脱细胞关节软骨基质内仍存在软骨蛋白聚糖。

Objective : To investigate expression of oncogene and its significance in benign and malignant preomorphic adenomas of salivary gland.

采用免疫组织化学方法对5例瘤旁腺体，21例良性多形性腺瘤和24例恶性多形性腺瘤中C-H-ras和P53蛋白产物的表达进行形态学观察。

Methods The expression of COX-2 and Survivin of 30 cases of SACC and 20 cases of normal salivary gland was examined with immunohistochemical technique.

采用免疫组织化学检测30例涎腺腺样囊性癌及20例正常涎腺组织中COX-2与Survivin蛋白的表达情况。

Transcription, western, and immunohistochemical analysis showed increased levels of dystrophin transcript and protein, and correct localization at the sarcolemma.

用免疫组织化学分析用纳米作为载体进行寡聚核苷酸递送的实验鼠抗肌萎缩蛋白基因RNA和抗肌萎缩蛋白的水平得到提高，而且恢复了抗肌萎缩蛋白在肌肉细胞膜上的正确位置。

Methods:Immunohistochemistry and image analysis methods were conducted to examine the expressions of EGF,TNF-α and TGF-β1 in both groups at 3,5,7,9 and 14 days after scalding.

用免疫组织化学方法结合图像数据分析，分别在烫伤后第3、5、7、9、14d检测EGF、TNF-α、TGF-β1表达水平的变化。

Objective: To investigate the effects of sulindac on the growth and motility of ovarian cancer cell SKOV3 and its involved mechanism. Methods: In order to detect the effects of sulindac on cultured SKOV3, sulindac with different concentration and untreated control were set up. The changes in morphology of SKOV3 were observed by phase contrast microscopy. MTT colorimetric assay was used to study the effects of sulindac on SKOV3 cell growth. Motility of SKOV3 was determined by scarification assay.

目的：研究舒林酸对卵巢癌SKOV3细胞的生长和细胞运动的影响以及对基质金属蛋白酶2（MMP2）、环氧合酶-2(COX-2)表达的影响：方法：将不同浓度的舒林酸加入培养液中观察其对SKOV3细胞形态的影响，采用MTT法观察舒林酸对SKOV3细胞生长的影响，通过划痕试验观察舒林酸对SKOV3细胞运动的影响，利用免疫细胞化学方法研究舒林酸对SKOV3细胞MMP2、COX-2表达的影响。

Methods: in order to detect the effects of sulindac on cultured skov3, sulindac with different concentration and untreated control were set up. the changes in morphology of skov3 were observed by phase contrast microscopy. mtt colorimetric assay was used to study the effects of sulindac on skov3 cell growth. motility of skov3 was determined by scarification assay.

目的：研究舒林酸对卵巢癌skov3细胞的生长和细胞运动的影响以及对基质金属蛋白酶2（mmp2）、环氧合酶-2(cox-2)表达的影响：方法：将不同浓度的舒林酸加入培养液中观察其对skov3细胞形态的影响，采用mtt法观察舒林酸对skov3细胞生长的影响，通过划痕试验观察舒林酸对skov3细胞运动的影响，利用免疫细胞化学方法研究舒林酸对skov3细胞mmp2、cox-2表达的影响。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。