免疫化学

Methods HB-EGF protein in active psoriatic lesions before and 10 days after the treatment of tazarotene was detected by immunohistochemistry.

用免疫组织化学的方法检测维甲酸诱导前后HB-GFE在进行期银屑病皮损中的表达。

The results were as follows:(1) The CGRP- positive fibers were found in the middle and caudal part of the striatum, and located between the caudate putamen and pallidum, corresponding to the MrD of the striatum.

应用免疫细胞化学ABC法对降钙素基因相关肽在大鼠纹状体边缘区内的分布以及与杏仁核、终纹床核等边缘系统结构之间的联系进行了研究。

Methods:The expression of CK20mRNA in portal venous blood from 30 colorectal cancer patients was detected by fluorescent quarto RTPCR,and the results of CD44v6 and PCNA in colorectal cancer tissue were determined by means of immunohistochemistry,and then compared with control groups through statistics analysis.

应用荧光定量RTPCR法检测50例大肠癌患者回流门静脉血中CK20mRNA，同时应用免疫组织化学方法测定大肠癌癌组织中CD44v6、PCNA的表达，并与对照组比较。

The blank group, the pure chondrogenic inductor group and the group of the G ranula of P enetrating Bone and Removing Pain mixed with chondrogenic inductor. We adopted pro-culture solution, pure chondrogenic induced culture solution （ TGF-β310ug/L , Dex10-7mol/L , VitC50mg/L ） and the chondrogenic induced culture solution which included the serum of the G ranula. All groups were cultivated in 50ml cell culture bottles. The effects of the G ranula of P enetrating Bone and Removing Pain on chondrogenic phenotype differentiation of BMSCs were investigated after being cultivated for 1, 2, 3 weeks, then cells observed by inverted phase contrast microscope and immunocytochemical stain.

3将第2代SD大鼠BMSCs分为空白对照组、单纯软骨诱导剂组及透骨消痛颗粒含药血清加软骨诱导剂组，采用原培养液、软骨诱导培养液（TGF-β310ug/L，Dex10-7mol/L，VitC50mg/L）及透骨消痛颗粒含药血清的软骨诱导培养液，50ml细胞培养瓶内进行培养，1、2、3周后通过倒置相差显微镜及免疫细胞化学染色等实验技术，研究透骨消痛颗粒对BMSCs诱导成软骨细胞的影响。

Results］The gross morphology of the tendons showed the experimental group had lesser adhesion and better reactiveness than that in the control group.The fibrin had degraded at 3 weeks.

分别于术后1周，3周，6周及12周对移植部位进行大体标本观察，细胞示踪、组织学检查、免疫组织化学检查、形态测定分析及生物力学测定。

Methods Review was done in 108 cases of children's bone LCH, using the envision way of immunohistochemistry dyeing on 40 cases, to reclassify them according to the WHO separate criteria made in 2000, then followed them up.

回顾性分析108例小儿骨LCH的临床资料，其中40例行免疫组织化学染色。

1.To study the expression of in the different glucose concentration with the time changing. 2.To study the effect of different concentration of Hepatocyte Growth Factor on the Integrin-linked kinase in protein and mRNA level by Mesangial Cells in high concentration (25 mmol/L) of glucose. 3.To study the effect of different concentration of HGF on the Fibronectin'secretary by Mesangial Cells. 4.To study latent therapeutic action and possible mechanism of HGF on the glomeruler sclerosis. The difference of ILK's experession on mRNA level in different concentration of glucose and HGF are detected by means of revers transcription-polymerase chain reaction,and by means of immunohistochemistry to detect their difference in protein level;the effection of HGF to the FN's secretary in high glucose are detected by means of enzyme linked innunosorbent assay.

1探讨在不同的血糖浓度下大鼠肾小球系膜细胞中ILK伴随时间变化在蛋白和mRNA水平表达的差异 2探讨不同浓度的HGF对高糖环境下(25mmol/L)大鼠肾小球系膜细胞中ILK在蛋白和mRNA水平表达的影响 3探讨不同浓度的HGF对高糖环境下大鼠肾小球系膜细胞的FN的分泌的影响 4初步探讨HGF对肾小球硬化的潜在的治疗作用及其可能机制采用RT-PCR的方法分析在不同血糖浓度和不同HGF浓度下大鼠肾小球系膜细胞中ILK在mRNA水平表达的差异；采用免疫组织化学的方法直观的观察它们在蛋白水平表达的差异；采用ELISA法分析不同浓度的HGF对高糖环境下大鼠肾小球系膜细胞的FN的分泌的影响。

Methods Mouse models of tibia fracture healing were established, and callus samples were collected 1, 3, 7, 14, 21 and 28 days after fracture. The development of callus and new bone formation were evaluated with roentgenology, Micro-CT and tetracycline double labeling method, and the expression of HIF-1α, vascular endothelial growth factor, Runx2 and ALP in callus were detected with RT-PCR, Western blotting and immunohistochemistry. The relationship between HIF-1α and fracture healing was analysed.

建立小鼠胫骨骨折模型，骨折后1、3、7、14、21、28 d取材，应用X线摄片、Micro-CT和四环素荧光双标记技术，观测骨痂组织的演变和新骨形成状况；采用RT-PCR、Western blotting和免疫组织化学方法，检测骨痂组织细胞HIF-1α、血管内皮生长因子和成骨性标志物（Runx2和ALP）的表达状况，分析HIF-1α与骨折愈合进程的关系。

Methods :The Alcianblue Safranin and immunohistochemical staining were employed to observe the density of MC, PCNA and oncogene bcl-2 expression in the specimens of 74 cases with gastric cancer.

采用阿尔辛蓝-沙红及免疫组织化学染色法，同步检测74例胃癌标本中的肥大细胞数及PCNA、bcl-2基因蛋白的表达。

The histological staining showed that toluidine blue, safranin O and anti-collagen II immunohistochemistry staining were positive.

组织学观察显示，三维多孔支架甲苯胺蓝染色、番红O染色、Ⅱ型胶原免疫组织化学染色均呈阳性。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。