免疫化学

Methods Epileptic animal models of adult Sprague-Dawley rats were induced by intraperitoneal injection of pilocarpine, which were divided into four groups including extrogenous NPY interference group.

方法将 SD大鼠随机分为 4组，采用匹鲁卡品癫痫动物模型及外源性 NPY侧脑室注入干预法，并以免疫组织化学染色观察 NPY的表达。

CARAPAX ET PLASTRUM TESTUDINIS/pharmacology ; REINFORCING KIDNEY ; MYELOID PROGENITOR CELLS ; CELL CULTURE ; IMMUNOHISTOCHEMISTRY

龟板/药理学；补肾；骨髓祖代细胞；细胞培养；免疫组织化学

Recently, it is proved that SP is also involved in pain modulation, acupuncture analgesia and immunoin- hibition. However, the role of CGRP in them is unclear Therefore, the dy- namic changes of CGRP-, SP-like immunoreactivity and expression ofα- CGRP,β-CGRP mRNAs in dorsal root ganglion and spinal cord in the arthritic rats were observed by using immunohistochemistry and in situ hybridization techniques combined with behavioral tests, respectively. Subsequently, the effects of this peoptide on the develoment of polyarthritis were investigated by intrthecal injections of CGRP and its antagonist in the rats. In addtion, GAL is a novel neuropeptide which coexists with CGRP and SP, but it may play a different role in the primary sensory system. Thus, we also observed the changes of GAL and GAL mRNA in DRG neurons and the spinal cord. The results were as follows

为此本文拟在大鼠佐剂性关节炎模型上，结合行为学测定、用免疫组织化学、原位核酸分子杂交技术观察脊髓背角及DRG中CGRP、SP、α-CGRP mRNA、β-CGRPmRNA的动态变化及电针的影响，此外，用鞘内和外周注射CGRP的工具药探讨了CGRP在佐剂性关节炎发生发展中的可能作用；甘丙肽（Galanin，GAL）也是新近发现的一个神经肽，并与CGRP和SP共存于DRG及初级传入末梢中，在神经损伤模型中脊髓CGRP、SP减少，GAL则明显增加，显示GAL与CGRP和SP的作用可能不同，因此在佐剂性关节炎模型上也同时观察了GAL和GAL mRNA的变化，实验结果如下

Methods: Using immunohistochemical methods detect the expressions of IL-3 in serum of 66 patients with nasal polyp and 22 normal people.

采用免疫组织化学方法检测66例鼻息肉患者血清标本和22例正常人血清标本中IL-3的水平。

In order to investigate the effect of IFN-γon the expression of TNF-αin the hypothala- mus-pituitary-ovary axisof RU486-induced aborted SD rats, RU486 was used to establish the abortion model, then the aborted rats were administrated with different doses of IFN-γintraperitoneal injection, the method of immunohistochemical ultrasensitive SP was us- ed to study the variation of positive TNF-αcells in hypothalamus, pituitary, ovary and uterus in normal pregnant group A, aborted model group B and three different doses of IFN-γgroup C,D,E. The main goal of this research was to study whether the low dose of IFN-γcould have a protective function to pregnancy and the mechanism of its function so as to provide morph- ologic basis for the scientific using of IFN-γin the process of animal abortion control.

为探讨IFN-γ对流产大鼠下丘脑-垂体-卵巢轴(hypothalamus-pituitary- ovary-axis，HPOA )TNF-α表达的影响，本试验用米非司酮(RU486)建立SD大鼠流产模型，通过腹腔注射不同剂量IFN-γ，采用超敏感免疫组织化学SP法(链霉素抗生物素—过氧化物酶法)，观察了孕13天(d13)SD大鼠正常妊娠A组、流产模型B组、不同剂量IFN-γC、D、E组下丘脑、垂体、卵巢、子宫TNF-α的表达变化，探讨低剂量IFN-γ对妊娠是否有保护作用及其作用机制，期望能为在妊娠过程中如何科学应用IFN-γ防止动物流产提供形态学依据。

Methods Immunnohistochemistry was used to detect the expression of Survivin protein in the trophoblasts of placenta which were taken from 10 samples of postdate labor and 10 samples of fullterm labor.

采用免疫组织化学方法对10例过期妊娠和10例正常足月妊娠产妇的胎盘滋养细胞层Survivin表达进行检测和对比研究。

As mvd was significantly correlated with surgical stage（p.01）,higher level of mvd tended to be related to poor prognosis（p.05）.conclusion mvd is correlated with tumor malignancy,infiltration and metastasis,and can be used as an important prognosticator of endometrial carcinoma.

mvd与子宫内膜癌恶性程度和浸润转移密切相关，是临床判断预后的重要指标。关键词：子宫内膜癌；血管生成；微血管密度；免疫组织化学；临床病理因素；预后

Cell survival and differentiation were studied with immunohistochemistry, Prussian blue staining, and HE staining.

采用免疫组织化学、普鲁士蓝和HE染色对脑组织切片进行干细胞存活、分化及病理学研究。

MethodsEarly embryos at different stages and uterus of the non-pregnant, and pregnant uterus of 1 to 8 days pseudopregnancy in mice were obtained.

方法取小鼠早期胚胎、动情期未孕、真孕及假孕1～8d小鼠子宫，利用免疫组织化学染色及图像分析法，检测p38MAPK在不同发育阶段早胚及围植入期子宫内膜中的表达和变化规律。

The aim of this study was to investigate PGT, 15-PGDH and CBR1 expression in themouse uterus during early pregnancy and their regulation in related models of pseudopregnancy,delayed implantation, artificial decidualization, steroid hormone treatment by in situ hybridization,RT-PCR and immunohistochemistry.

本研究利用原位杂交、免疫组织化学和RT-PCR等方法，检测了PG转运载体PGT、代谢酶15-PGDH和CBR1在小鼠早期妊娠子宫中的表达，并利用假孕、延迟着床及激活、人工蜕膜化、性腺类固醇激素处理等模型，研究了它们在小鼠子宫中的表达与调节。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。