免疫化学

METHODS摘要: Otocyst epithelia surgically isolated from fetal rats were cultured and passaged. Proliferation and differentiation of cultured cells were observed using BrdU labeling to find the progenitors. Immunocytochemistry (Factin, cytokeratin and calretinin) was used to detect cell properties of cultured cells.

方法摘要：取孕鼠胚胎听囊细胞进行体外培养，用免疫细胞化学方法，分别对培养的细胞进行细胞角蛋白、F肌动蛋白和calretinin染色观察细胞特征；BrdU染色观察细胞增殖。

We select 5 cirrhotic tissues and 5 HCC with their paired samples for tissue transplantation and cultured fibroblasts.

免疫组织化学染色ABC法检测三种组织样本中c-kit、CD133、CD90、CD34、CK18和CK-19等。

Methods Three cases of palisaded myofibroblastoma were studied by histopathology and immunohistochemistry, combining with the review of the literature.

对3例栅状肌纤维母细胞瘤进行组织形态学和免疫组织化学研究，结合文献对本病的诊断标准、鉴别要点及组织来源进行探讨。

Methods: A early model of brain radiation-induced injuries in the rat was developed. Analysis of the expression of TNF-α TNFR1 and TNFR2 in the pallium was done at different dose and time level after whole-brain irradiation with immunohistochemistry and computer technology.

在大鼠早期脑放射性损伤模型基础上，应用免疫组织化学方法染色标本，运用计算机图像技术计算并分析大鼠大脑放射性损伤后大脑皮质区在不同剂量、不同时间点TNF-α、TNFR1和TNFR2的动态表达。

There were also glands and extracellular materials in different stages of development. Immunocytochemical study showed that factor Ⅷ antigen and PALP were positive in vascular endothelium.

免疫细胞化学方法显示：正常流出或刮出组织的毛细血管内皮因子Ⅷ相关抗原和胎盘碱性磷酸酶呈阳性反应。

The samples were fixed with paraformaldehyde and embedded in paraffin.

应用免疫组织化学技术检测鼻粘膜组织中水通道蛋白1、2、3、4、5的分布情况。

Methods Healthy male SD rats of different age groups were recruited(1-, 2- and 3- week groups, 1- and 2- month groups, 6- month grown group, and 18- month aged group; n=5 each). Immunohistochemistry was performed on the coronal sections of brain of the rats perfused with paraformaldehyde.

健康雄性SD大鼠，分为7天、2周、3周、1月、2月、成年（6月龄）和老年（18月龄）7组，每组5只，共35只，多聚甲醛灌注固定，冰冻冠状切片，免疫组织化学染色，光镜观察摄像，细胞计数。

All of the specimens collected were divided into two parts, one part for immunohistochemical staining after fixated by 4% paraformaldehyde, the other part for culturing fibroblasts.

所有取材部位未经任何治疗，标本经无菌取材后分成2份，其中一份用40 g/L多聚甲醛固定后用于免疫组织化学检测，另一份用于成纤维细胞培养。

MAIN OUTCOME MEASURES: Neurologic function rating, pathological changes, volume of hematoma and immunohistochemistry of these two groups.

主要观察指标：两组大鼠神经功能学评分，病理变化，血肿大小，免疫组织化学染色结果。

METHODS: 30 days and 60 days after being cutting olfactory peduncle,the rats were put to death by perfusion for fixation.

随机分为嗅茎切断30 d组(n=10)和60 d组(n=15，其中5只作免疫组织化学)。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。