免疫化学

All rats were killed 8 days after operation and the expression of transforming growth factor-13 was detected by immunohistochemical method.

术后第8天分别处死各组大鼠，用免疫组织化学方法测定转化生长因子日的表达情况。

Six of rats in each group were sacrificed at days 2, 7, and 14 after operation, and the expression of Fos protein in the spinal cord were assayed with the immunohistochemical methods and image analysis.

于术后第2，7，14天各组分别处死大鼠各6只，取脊髓，应用免疫组织化学方法和图像分析系统检测脊髓中Fos蛋白的表达。

Results The number and optical density of Bax in hippocampus of PTZ group rats was higher than that of control group rats.

然后处死大鼠取脑，免疫组织化学的方法检测海马神经元Bax和Bcl-2蛋白的表达。

Computer image analysis was used to measure the average optical density of synaptophysin.

方法采用免疫组织化学方法，观察突触素在不同周龄的人胎儿海马中的表达水平，利用计算机图像分析技术测量不同周龄阶段海马突触素表达的平均光密度。

Methods: The effects of Opuntia on proliferation of fibroblast were measured with MTT colorimetric assay, and PCNA productions were examined with immunocytechemistry.

以小鼠成纤维细胞株(NIH3T3)为研究对象，采用四氮唑盐比色法测定仙人掌对成纤维细胞增生的影响，采用免疫细胞化学染色法检测成纤维细胞的增殖细胞核抗原的表达。

The journal publishes reviews covers the whole field of the biology and biochemistry of cancer, emphasizing oncogenes and tumor suppressor genes, growth-related cell cycle control signalling, carcinogenesis mechanisms, cell transformation, immunologic control mechanisms, genetics of human cancer, control of cell proliferation, genetic and molecular control of organismic development, rational anti-tumor drug design.

该杂志主要发表有关肿瘤生物学和生物化学的综述。其内容包括：致癌基因和肿瘤抑制基因，与生长有关的细胞周期控制的信号转导，致癌机理，细胞转化，免疫控制机理，人类癌症遗传学，细胞增殖的控制，组织发育的遗传和分子控制，合理的抗肿瘤药物设计等。

Methods: By using immunohistochemistry method, the expression of Survivin in osteosarcoma, osteochondroma and normal osseous tissue, and the expression of P-glycoprotein in osteosarcoma was detected.

采用免疫组织化学S-P法检测凋亡抑制基因Survivin在骨肉瘤、骨软骨瘤和正常骨组织中的表达及P-糖蛋白在骨肉瘤中的表达。

Methods The expression of survivin was detected by S-P immunohistochemical technique in 46 cases of human osteosarcoma.

应用免疫组织化学S-P法对46例原发性骨肉瘤组织中survivin蛋白的表达水平进行检测。

The present study is to culture otocyst cells in fetal, cochlear sensory epithelial cells of neonatal and mature respectively in vitro with refinement of culture media and techniques. Immunocytochemistry and Bromodeoxyuridine labeling are used to detect properties and mitotic status of cultured cells. With the observations about proliferation and differentiation in cultured cells, we want to find the origin of regenerative cells and to understand how these progenitors proliferate and differentiate.

本研究分别对胎鼠听囊、新生鼠听觉上皮和成年鼠听觉上皮用自然培养方法进行体外培养，并应用免疫细胞化学方法，对培养细胞进行染色标记和观察，系统了解哺乳动物听觉细胞发生、发育过程，以及这些细胞在体外的增殖和分化过程，旨在了解哺乳动物听觉细胞听觉上皮感觉细胞的来源，发现细胞生长的前体细胞。

METHODS: Otocyst epithelia surgically isolated from fetal rats were cultured and passaged. Proliferation and differentiation of cultured cells were observed using BrdU labeling to find the progenitors. Immunocytochemistry (Factin, cytokeratin and calretinin) was used to detect cell properties of cultured cells.

取孕鼠胚胎听囊细胞进行体外培养，用免疫细胞化学方法，分别对培养的细胞进行细胞角蛋白、F肌动蛋白和calretinin染色观察细胞特征；BrdU染色观察细胞增殖。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。