免疫化学

The differentiation of the cells and the expression of Myosin Ⅶa were revealed by immunohistochemical staining.

结果脂质体介导的基因可在细胞内获得良好的表达，转染后24h，相差显微镜下观察细胞形态发生变化，免疫组织化学显示转染后细胞可以表达毛细胞特异性蛋白Myosin7a。

Some of the laryngeal MFH were difficult to be differentiated from carcinosarcoma, pleomorphic rhabdomyosarcoma , myxoid liposarcoma ,malignant neurilemmoma and myxoma without immunohistochemical aids .

喉恶性纤维组织细胞瘤常规组织学与癌肉瘤、多形性横纹肌肉瘤、粘液型脂肪肉瘤、恶性神经鞘瘤、粘液瘤有时易混淆，免疫组织化学有助于明确诊断。

Methods Human pancreatic carcinoma cell lines (n=4) were studied by using cell culture, immunocytochemistry and Northern blotting analysis.

采用细胞培养、免疫细胞化学和Northern印迹分析方法。

Methods Immunohistochemistry of streptavidin-perosidase method was applied in the observation of the expression of TIAF-1 in each group, including tissues obtained from the unaffected part of nephrectomized kidneys with renal cell carcinoma (n=6) and cadaveric donor of normal kidneys (n=8) as the control group, tissues with acute rejection (AR, n=16) and those with chronic rejection (CR, n=28), and in the analysis combined with the number of positive cells of CD3, CD20 and CD68 infiltrated in the interstitium.

采用SP免疫组织化学染色法对6例因肾细胞癌行肾切除的未受肿瘤侵犯的正常肾组织和8例供肾正常肾组织、16例急性排斥反应和28例慢性排斥反应移植肾组织中TIAF-1的表达进行观察，并对间质浸润细胞中CD3、CD20、CD68阳性细胞数进行分析。

We observed 46 cases of lung cancer and 40 cases of adjacent non neoplastic tissues through SP immunohisto-chemical method.

采用免疫组织化学SP方法对 4 6例肺癌和 4 0例癌旁组织中E -cd表达进行研究。

PC12 cells were exposed to RA with different concentrations for 72 h and the lengh of neurite and max diameter of PC12 cells were observed at 24,48,and 72 h respectively. The expression of MAP2 was detected by immunocytochemistry after PC12 cells were exposed to RA for 72 h.

不同浓度的RA组均诱导PC12细胞72 h，在24 、48 和72 h分别观察细胞的突起长度和细胞最大直径的变化情况；在诱导72 h后利用免疫细胞化学技术，观察不同剂量RA作用后PC12细胞向MAP2阳性细胞分化的情况。

PC12 cells were exposed to NGF with different concentrations for 72 h and the lengh of neurite and max diameter of PC12 cells were observed at 24,48,and 72 h, respectively. The expression of MAP2 was detected by immunocytochemistry after PC12 cells were exposed to NGF for 72 h .

不同浓度的NGF组均诱导PC12细胞72 h，在24、48和72 h分别观察细胞的突起长度和细胞最大直径的变化情况；在诱导72 h后利用免疫细胞化学技术，观察不同剂量NGF作用后PC12细胞向MAP2阳性细胞分化的情况。

The CNTF and FGF2 positive cell number and the positive area percentage, as well as the number of neuron and neuroglia calculated by immunofluorescence analysis in the spinal cord.

将嗅鞘细胞移植入脊髓半横断SD大鼠，观察术后7天和21天嗅鞘细胞在脊髓内的存活情况，并对脊髓组织切片进行CNTF和FGF2免疫荧光组织化学染色，计数阳性细胞并计算阳性面积百分比。

Immunocytochemical staining was conducted for the neural stem cells, neuron and neuroglia specific cell surface marker such as nestin, MAP-2, NSE and GFAP.

免疫细胞化学方法检测诱导前后神经前体细胞、神经元和神经胶质细胞特异标记物如nestin，MAP-2，NSE和GFAP的表达。

Methods ①In situ hybridization was applied to observe the changes ofhypothalamic CRHmRNA positive cells after PX and melatonin supplementation;②Immunohistochemistry was used to observe the changes of CRH positive cellsin hypothalamus and CRH staining in median eminence of neurohypophysis after PXand melatonin supplementation;③ELISA was applied to detect the changes of CRHconcentration in serum after PX and melatonin supplementation.

①运用CRHmRNA 原位杂交的方法来观察松果体摘除及补充外源性褪黑素后下丘脑室旁核CRHmRNA 阳性细胞的变化；②应用免疫组织化学染色的方法观察松果体摘除及补充外源性褪黑素后下丘脑室旁核CRH 阳性细胞和神经垂体正中隆起CRH 阳性染色的变化；③运用ELISA 法检测松果体摘除及补充褪黑素后血清CRH 浓度的变化。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。