免疫化学

Methods The study was performed concering the neurons and fibers of SP,CGRP and VIP-immunoreactive substance in three predominant superfical ganglial plexus of the guinea pig heart by immunohistochemical ABC method and observed by light microscope.

应用免疫组织化学ABC法，对豚鼠心脏表面3个主要神经节丛的神经元及神经纤维进行了SP、CGRP及VIP免疫组织化学染色及光镜观察。

Despite evidence implicating prostaglandins as critical factor in male reproductive functions, little is known about expression and function of COX-2 in the male reproductive system. By using immunohistochemistry, RT-PCR and Western-blot, the content of this research included:(1) The expression and localization of COX-2 in mature rat and human testis were examined.(2) We investigated the changes of spermatogenesis and testosterone levels in testis after by using the COX-2 specific inhibitor-rofecoxib at 2, 4 and 8 weeks.(3) To explore the relationship between the intratesticular level of testosterone and expression of COX-2 by completely destroying the Leydig cells of mature male rats with injection of a single I.

本课题应用免疫组织化学、RT-PCR和Western-blot等方法检测以下内容：(1)在mRNA和蛋白水平检测正常成年男性及大鼠睾丸组织中COX-2的表达及定位情况；(2)实验组大鼠持续给予COX-2酶的特异性抑制剂Rofecoxib，分别于用药后2周、4周和8周检测大鼠的精子生成以及睾丸组织内睾酮浓度的变化；(3)另一组实验大鼠给予特异性Leydig细胞灭活剂EDS，在特定时间内抑制睾丸组织内合成的睾酮，于用药后2周和4周，采用免疫组织化学及RT-PCR方法观察大鼠睾丸组织内睾酮浓度变化对COX-2合成的影响。

According to previous studies, the hosts —parasites immune interactions were investigated in rabbits following immunization with extracts from Ixodes sinensis basing on medical entomology, immunology, biochemistry, pathology and histochemistry and reported from the following eight fields

本研究在以往研究工作的基础上，首次选用我国江南地区具有重要医学和兽医学意义的优势蜱种：中华硬蜱的不同抗原对新西兰兔进行免疫接种，对宿主—寄生虫免疫相互作用从医学昆虫学、免疫学、生物化学、病理学、组织化学等不同方面进行研究，就以下8个方面首次进行报道。

There were 8 patients in the macromastia group and 5 patients in the control group. The result didn't show significant difference between the two groups. There is no difference between the two groups in mammary epithelial cell size measurement and Akt-1/2/3 immunohistochemistry stain. The levels of Ki-67, phospho-Akt-1/2/3, and STAT5a of juvenile macromastic patients are higher than those of the patients of the control group, and the results are statistically significant.

三年内共搜集到八位巨乳症病患及五位对照组，两组以Wilcoxan rank sum test比较，乳腺上皮细胞的尺寸及Akt-1/2/3免疫组织化学染色方面在统计学上的没有显著的差异，而乳腺上皮细胞之Ki-67、phospho-Akt-1/2/3、及STAT5a免疫组织化学染色方面，则均为青春期巨乳症患者组较高，统计学上均有显著性的差异。

Recombinatino polypeptide or chemically synthesized polypeptide antigen is used to immunize the mammal to obtain polyclonal antibody and the nonconservative amion acid sequence of the antiboby to N-terminal with recognition site qBrn-2 of development regulatory protein qBrn-2. The antibody does not recognize other POU structure domain protein, and recognizes specifically qunique protein in Western imprinting experiment. The qBrn-2 expression mode obtained immunohistochemistry process is identical with the resulted height of in-situ molecular hydridization.

以重组多肽或化学合成多肽为抗原免疫哺乳动物获得的多克隆抗体，这一抗体对发育调控蛋白qBrn－2的识别位点为qBrn－2的N－末端的非保守性的氨基酸序列，该抗体不识别其它POU结构域蛋白；在Western印记实验中，该抗体特异地识别单一的蛋白；利用免疫组织化学接示的qBrn－2表达模式与分子原位杂交的结果高度一致。

Compared with those in control group,the number of positive immunoreactivity cell in RSR and intestine increased and its intensity of immunoreactivity enhanced obviously after rabbit was infected with Pasteurella multocida.

免疫组织化学观察发现，产抗菌肽细胞在兔圆小囊的黏膜上皮、圆顶上皮以及淋巴组织的滤泡生发中心、圆顶区和帽区均有分布；在兔感染多杀性巴氏杆菌后，圆小囊及其他肠道组织中的抗菌肽免疫组织化学阳性细胞数量明显增多，阳性反应增强。

After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法：1、培养原代的人RPE细胞，经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后，通过AO/PI染色技术确定培养细胞的存活率，描记其生长曲线，第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后，通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后，采用MTT法观察其对细胞的增殖力的作用；通过流式细胞仪观察其对细胞周期的影响；通过扫描电镜观察其对细胞形态的影响；通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用；采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率，评价其对细胞间通讯功能的影响；通过制作RPE细胞损伤模型，观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质，应用等电聚焦电泳和SDS-PAGE双向电泳技术，银染显示分离出的蛋白质斑点，经凝胶图像分析软件对两个样本进行胶图分析，寻找差异蛋白点。

The latency of the waves of SEP or MEP is extracted. But it still is the best in all. The control group is the worst of all. The wave can be found again after the spinal cords are hemisectcd in control group. 5.. Seen from the immunohistochemistry of NF, Schwann cells can promote neural dendrite outgrowth toward the area of hemisected spinal cord.

神经纤维的兔疫组化染色脊髓半切损伤后，经NF的免疫组织化学技术染色显示：移植雪旺细胞可以促进神经纤维向脊髓损伤区域生长，抗体封闭 LI后神经纤维的生长受到抑制，再生神经纤维数相对减少，这从免疫组织化学方面说明口对神经再生具有影响。

A 46-year-old man presented with a gradually growing nodule on the left dorsal foot, which had been present for over 10 years. In the beginning, the lesion was treated as a keloid. Because of poor response to treatment, biopsy was arranged. Sections showed a dermal tumor composed of numerous foamy cells, hyalinized wiry collagen, spindle cells and entrapped collagen. The foamy cells were positive for KP1(CD68) and negative for S-100 protein and HMB-45. The histological and immunohistochemical results were consistent with the diagnosis of lipidized fibrous histiocytoma.

一位46岁男性从十年前开始於左足背上出现一个逐渐长大之结节，一开始被当作蟹足肿给予治疗，因为对治疗的反应不好，故安排了切片检查，病理显示一个由大量泡沫状细胞，坚硬而透明之胶原纤维，梭状细胞，胶原纤维束所构成的真皮肿瘤，免疫组织化学染色显示KP1(CD68)阳性，S-100 protein与HMB45阴性，综合病理与免疫组织化学染色结果，诊断为脂肪性纤维组织细胞瘤。

objective:to investigate the expression and significance of rb in the occurrence, development and regression of infantile hemangiomas.methods:the expression of rb was examined in the proliferative stage and catagen of human hemangiomas and normal skin tissues by using immunohistochemical technique.immunohistochemical technique for factorⅷ-related antigen was used to prove that the cells which expressed rb were endothelium.image analysis system was applied to measure the expression level of rb at different stages of hemangiomas and in normal skin tissues.results:the expression of rb was significantly lower in proliferating hemangiomas than that in involuting hemangomas(p.05).conclusion:rb might play an important role in the regression of human hemangioma endothelial cells and anti-angiogenesis.

目的：探讨rb蛋白在血管瘤发生、发展及退化过程中的表达状况及其意义。方法：采用免疫组织化学方法检测人皮肤血管瘤增生期、退化期及正常皮肤组织中rb的表达水平，并结合第ⅷ因子相关抗原的免疫组织化学染色证实表达rb的细胞是血管内皮细胞。利用计算机图像分析技术测量不同时期血管瘤组织和正常皮肤组织rb表达的积分光密度和面积。结果：增生期血管瘤内皮细胞rb表达水平低于退化期，差异有显著性（p.05），退化期血管瘤内皮细胞rb表达水平与正常皮肤组织相比，差异有显著性（p.05）。结论：rb通过抑制血管瘤内皮细胞增殖和血管生成而在血管瘤的退化过程中起重要作用。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。