免疫化学

Results:In experimental group,all 20 rats have unifocal masses and S-100 protein without extracerebral metabasis and the extracranial extension. The size, the site and morphology of all tumors are almost the same.

结果：脑胶质瘤组，20只大鼠经MRI平扫、增强扫描和形态学检查证实均有单灶肿块，且肿瘤部位、大小、形态基本一致，无脑外转移和颅外扩散，经免疫组织化学染色检测S-100蛋白均为阳性。

Objective To explore livin, MTA1 and the caspase3 protein expression, their correlation and clinical pathology in colon cancer Methods The expressions of livin, MTA1 and caspase3 protein in 88 cases of colon cancer were detected by immunohistochemistry stainingResults Livin, caspase3 protein and MTA1 positive expression rates in colon cancer tissues were more than those in tissues beside the cancer Furthermore there were obvious relevance between livin protein, MTA1 positive expression rate and degree of histodifferentiation and lymph node metabasis and between caspase3 protein positive expression rate and degree of histodifferentiation of colon cancer Caspase3 protein expression had prominent inverse correlation with the livin expressionConclusions ①Over expression of livin having inhibtion on colon cancer is one of important factors of colon carcinogenesis ②Caspase3 protein expression in colon cancer tissues is inhibited to less and has prominent inverse correlation with livin expression Accordingly, suppressing caspase3 protein activity is one of channels, by which livin promotes colon carcinogenesis ③MTA1 plays the important role in histodifferentiation degree and lymph node metastasis of colon cancer

采用免疫组织化学染色方法检测88例结肠癌组织中Livin，Caspase3 及MTA1的表达情况。结果（1）结肠癌组织中Livin的阳性表达率显著高于癌旁组织，且Livin的表达与结肠癌的组织分化程度及淋巴结转移程度显著相关。（2）结肠癌组织中Caspase3蛋白的阳性表达率显著高于癌旁组织，且Caspase3蛋白的表达与结肠癌的组织分化程度显著相关。（3）结肠癌组织中MTA1的阳性表达率显著高于癌旁组织，且MTA1的表达与结肠癌的组织分化程度及淋巴结转移程度显著相关。（4）Caspase3蛋白的表达又与Livin的表达呈显著负相关。结论（1）Livin在结肠癌组织中过表达，它可能是促进结肠癌发生的重要因素之一。（2）Caspase3蛋白在结肠癌组织中表达被抑制而降低，且与Livin的表达呈负相关。因此，抑制Caspase3蛋白的活性是Livin促进结肠癌发生的途径之一。（3）MTA1对结肠癌的组织分化及淋巴转移发挥重要作用。

The expression of proliferating cell nuclear antigen in each group was detected by immuno-histochemistry method. The cell cycle distribution was measured by flow cyto- metic analysis.

MTT法及免疫组织化学染色增生细胞核抗原法检测细胞生长活性，流式细胞仪测定细胞周期时相变化。

METHODS: Spinal cord injury model produced in 42 Wistar rats on T7 by use of modified Allen's impact method (10 g x 5 cm); 3 days after injury, 20 microliters FSCS with a density of 1 x 10(5)/microliter prepared from E14 rat were injected into the epicenter of the traumatized cavity.

方法对 4 2只 Wistar成年大鼠以改良 Allen法(10 g× 5 cm)打击脊髓，3天后将孕 14天(E14 ) 5只胚胎脊髓细胞悬液 2 0 μl植入损伤空腔，移植后 2、4、6、8、10和 12周，以组织学、免疫组织化学观察移植物成活、分化及其与宿主之间关系。

Rat AAA model was made by perfusion PPE elastase through the abdominal aorta, and the specimen was obtained on postoperativeday 3, 7, 14, 28, respectively. Specific elastic fiber staining was used to observe the disruption of elastic fiber in aortic wall. Immunohistochemistry staining of CD68 was applied to observe the microphage infiltration. The in tisu hybridization and western blot of MCP-1 and MMP-2 were used to study the expression of mRNA and protein. Thus we can analyze the facilitated function of MCP-1 gene to the microphage adherence and infiltration on the earlier AAA.

采用经腹主动脉腔内导管灌注的方法制成大鼠AAA模型，分别于术后3天、1周、2周、3周、4周获取各组大鼠腹主动脉标本，行弹力纤维特殊染色观察动脉壁弹力纤维的破坏情况；行CD68免疫组织化学染色观察动脉壁中巨噬细胞的浸润；行MCP-1及MMP-2的原位分子杂交、Western蛋白质印迹观察二者的mRNA表达和蛋白表达；分析MCP-1基因促进巨噬细胞的黏附、浸润在早期AAA发病中的作用。

Methods AD model was established by injecting β amyloid protein into the hippocampus of rat. The rats were killed ten days after injection. The hippocampus sections were made coronally on a freezing microtome.

采用海马注射A淀粉蛋白的方法建立AD模型。10d后对大鼠进行灌注，取脑，冰冻切片，用NGF、BNDF和NT3抗体行免疫组织化学染色。

A total of 24 g of tissue was removed, and in 4 of 17 blocks submitted a lesion morphologically and immunohistochemically similar to clear cell carcinoma of the kidney was noted.

共有24克的组织，在被移除 17块的形态学和免疫组织化学的提交了病变类似于透明细胞癌的肾脏引起了我的注意。

Expression of iNOS in rat kidney tissue was detected by immunohistochemitry and morphometry computer image analysis.

应用免疫组织化学方法和图像分析系统检测肾组织中INOS的表达和分布，比较各组血清肌酐、尿素氮，同时观察肾脏组织形态学改变。

With the development of physiology,immunohistochemistry and application of electron microscope,researchers want to know the ultramicrostructure,3D ultramicrostructure and physiologic function of ICCs,to think highly of the effect of ICC,and try to interpret morphosis and physiologic function about it.

随着电镜的应用及生理学、免疫组织化学的发展，人们对Cajal间质细胞的超微结构、三维网络结构及生理功能有了进一步的认识，开始重视Cajal间质细胞的作用，试图阐明它们的形态结构和生理功能。

Besides, this coronavirus is a new kind of virus which belongs to a mutate

够产生某种化学物质而导致我们的免疫细胞死亡。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。