免疫化学

Number of neural stem cells is small. NSCs look like circle or ellipse with or without short neurite, with large nuclear-cytoplasmic ratio and deep karyotin. NSCs have no visible differences with other kinds of cells in appearance, and have cell surface marker. Currently, there are mainly three aspects to identify NSCs: expression of specific nerve antigen, including nestin, Musashi, transcription factor, and cell adhesion molecules; self-renewal ability including single cell clone analysis, BrdU mark and S phase cell; the potential of multi-direction differentiation including immunocytochemical process and reverse transcriptase polymerase chain reaction.

由于神经干细胞的数量很少，从细胞形态来看神经干细胞为圆形或椭圆形，无或有较短的突起，核质比大，核染色较深，形态上与其它种类的细胞没有明显的差异，并且未找到一种细胞表面特异性标志物，因此目前鉴定神经干细胞主要有以下3个方面：特异性神经抗原的表达，包括巢蛋白、Musashi、转录因子及细胞黏附分子；自我更新能力，包括单细胞克隆分析、BrdU标记S期细胞；多向分化潜能，包括免疫细胞化学法、聚合酶链反应色法。

Methods In situ method of terminal deoxytransferade-mediated dUTP-biotin nick and labelling staining was used to identify programmed cell death in 20 KAs. These cases also were examined with immunohistochemical technique for the expression of two protooncogenes, bcl-2 and bax, recently recognized to be involved in apoptosis.

用末端脱氧核苷酰转移酶介导的d-UTP生物素缺口末端标记技术，原位检测20例角化棘皮瘤中凋亡细胞；应用免疫组织化学技术检测皮损部位bax和bcl-2基因产物的表达。

Methods: 21 buttons of keratoconus, 7 buttons of normal cornea were detected.

材料与方法：取角膜移植术钻下的圆锥角膜组织21例，无眼病的尸体角膜7例，全部标本为石蜡包埋，免疫组织化学采用SABC法。

Methods Immunohistochemistry was utilized to measure the expression of AQP3 in tissue samples from 30 patients with seborrheic keratosis, 15 patients with Bowen′s disease, 32 patients with squamous cell carcinoma, 17 patients with malignant melanoma and 15 normal human controls.

应用免疫组织化学方法检测30例脂溢性角化病、15例Bowen病、32例鳞状细胞癌、17例恶性黑素瘤及15例正常人皮肤组织中AQP3的表达。

Methods The immunohistochemical method was used to detect the expression of GST-P in LEC rat liver.

采用免疫组织化学方法检测LEC大鼠肝脏GST-P的表达。

BHP manufactures light-detectors and medical instruments such as SPECT, gamma-Camera, luminometer, etc.

北京滨松主要生产光探测器件和医疗设备(SPECT、γ相机和化学发光免疫分析仪等)。

Immunohistochemical localization of inhibin α-subunit in normal mature rats was carried out by using Strept Avidin-Biotin-Peroxidase Complex .The results showed that there was positive immunoreactivity of the McAb to inhibinα-subunit in heart ,kidney , spleen , lymph ,adrenal gland and pancreas cells. It indicated that inhibinα-subunit existed in the above tissues , But no positive sites were seen in hypothalamus, pituitary, lung and liver, that means they have no inhibinα-subunit. Compared with the ovariectomized rats, the hypothalamus and pituitary of the rats injected with inhibin-α fragment（1～32）showed an positive reaction, suggesting that inhibin α-subunit could pass through the blood-brain barrier .

用链霉亲和素-生物素-过氧化物酶(Strept Avidin-Biotin-Peroxidase Complex， SABC)免疫组织化学方法，以抑制素α亚基单克隆抗体对正常雌性性成熟大鼠各组织的抑制素α亚基进行定位观察，结果显示：⑴大鼠心脏、肾、脾、淋巴、肾上腺、胰腺均呈阳性反应，表明存在抑制素α亚基；下丘脑、垂体、肝脏和肺组织呈阴性反应，表明其不存在抑制素α亚基；⑵与去卵巢大鼠相比，外源注射抑制素α（1～32）片段的大鼠其下丘脑和垂体出现阳性反应，说明抑制素α亚基能通过血脑屏障。

So has the use of vitamin A to strengthen children's immune systems, and chemically treated bed nets to prevents malarial.

而且，应用维生素A来加强孩子的免疫系统，用化学的方法来处理基础网状物以预防疟疾。

MATERIALS AND METHODS: The protein expressions of MMP-7 and MMP-9 were evaluated by immunohistochemistry in 18 normal villi. 49 hydatidiform moles, in which 8 were malignantly transformed moles.

材料与方法：采用免疫组织化学PV-6000法测定49例葡萄胎（其中8例恶变，列为葡萄胎恶变组）和18例正常绒毛组织中的MMP-7和MMP-9蛋白表达量。

Methods The activity of PTK, PKCα,βI ,βII ,γ,ε,ξ and the active station of NF-κB in large bowel carcinoma tissues was detected by using membraneous conjugated method, laser scanning confocal microscopy and immuriohistochemistry respectively in the animal model of nude mouse transplantation tumors of large bowel carcinoma.

以大肠癌裸鼠移植瘤为动物模型，用膜结合法、激光共聚焦显微镜和免疫组织化学分别测定了大肠癌组织蛋白酪氨酸激酶、蛋白激酶Cα、βI、βⅡ、γ、ε和ξ的活性以及核因子-κB的活化状况。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。