免疫化学

Methods The β-catenin expression was examined immunohistochemically in intradermal nevus and malignant melanoma with β-catenin antibody.

应用免疫组织化学PowerVisionTM二步法检测25例皮内痣、45例恶性黑素瘤中β-catenin的表达情况。

Objective: To investigate the effect of intraperitoneal thermochemotherapy on T??

目的：探讨腹腔热化学治疗对机体细胞免疫水平的影响。

The times of penis intromission into vagina of estrous female rats during 30 minutes observation and intracavernosal pressure during erection resulting from stimulation of the autonomic innervation of the penis were measured after 2 weeks and 2 months of the operation.

应用内皮细胞特异性标志物CD-31分子和平滑肌特异性标志物α-actin对处理后2周的大鼠阴茎海绵体组织行免疫组织化学染色，了解雄激素对大鼠阴茎组织血管内皮细胞和平滑肌细胞的影响。

The immunohistochemical findings suggest that JIA-associated nongranulomatous iridocyclitis is a primarily B-cell-infiltrative process.

免疫组织化学结果表明JIA并发的非肉芽肿性虹膜睫状体炎主要是B细胞浸润过程。

The recipients were divided into three groups:allograft control group, allograft experimental group and isograft control group. After 60 days of transplant, vascular intimal thickness in all of the groups was observed by histological examination. The expression of PCNA and α-SMA was determined by immunohistochemistry.

动物分为3组：(1)异系移植对照组；(2)异系移植实验组；(3)同系移植对照组。60d后对移植动脉行病理组织学检测，观察移植动脉内膜增生程度，并行免疫组织化学染色检测移植动脉增殖细胞核抗原和平滑肌肌动蛋白的表达情况。

The mice were divided into three groups: a cardiac isograft of C57BL/6 to C57BL/6 mice; b cardiac allograft of Balb/c to C57BL/6 mice; c cardiac allograft of Balb/c to C57BL/6 mice with mfgl2 polyclonal antibody treatment.

采集移植心组织标本作病理学检查，以免疫组织化学方法测定mfgl2在移植心脏组织细胞上的表达，并对mgfl2的表达进行半定量分析。

METHODS: The models of abdominal aorta transplantation were made with micro-surgery in rats. The recipients were divided into three groups: allograft control group, atorvastatin-treated group and isograft control group. Vascular intimal thickness in all of the groups was observed by histological examination. The expression of proliferation cell nuclear antigenl and α-smooth muscle actin were determined by immunohistochemistry. The content of nitric oxide was measured by nitrate reductase chromatometry.

建立大鼠腹主动脉移植慢性排斥反应模型，分为3组：异系移植对照组、异系移植治疗组和同系移植组，60d后对移植动脉行病理组织学检测，观察移植动脉内膜增生程度；并行免疫组织化学染色，检测移植动脉增殖细胞核抗原和平滑肌肌动蛋白的表达情况，行硝酸还原酶比色法测定移植动脉组织NO的含量。

Quantitative analysis of bFGF and colorized intensity were detected with immunohistochemical stain and JVC imaging analytic system at 7, 14, 21, 28, 42 and 56 days after operation.

术后7，14，21，28，42，56d采用免疫组织化学染色和JVC图像分析处理系统行碱性成纤维细胞生长因子定量分析和显色强度判定。

Although many efforts have been made to elucidate the role of Kail gene in many kinds of carcinoma,there are little reports concerning the role of Kail in human colorectal carcinoma,especially regarding the mechanism of its down-regulation in metastasis carcinoma. In present study,the expression of Kail was detected by in situ hybridization,immumohistochemistry and Western Blot in four cell lines of HCC with different metastatic potential.

为探讨Kai1基因与大肠癌转移之间的相关性，本研究利用四种不同转移潜能的大肠癌细胞系HCT116、HT29、LoVo与SW480，从mRNA与蛋白质水平，用原位核酸杂交、免疫组织化学与Western Blot三种方法检测Kai1／CD82的表达，得到了一致的结果：Kai1／CD82在HT29与SW480细胞系的表达较LoVo与HCT116为高。

Fund Project: the National Natural Science and Technology Source Program, No. 2001DEA1006*Abstract: Number of neural stem cells is small. NSCs look like circle or ellipse with or without short neurite, with large nuclear-cytoplasmic ratio and deep karyotin. NSCs have no visible differences with other kinds of cells in appearance, and have no cell surface marker. Currently, there are mainly three aspects to identify NSCs: expression of specific nerve antigen, including nestin, Musashi, transcription factor, and cell adhesion molecules; self-renewal ability including single cell clone analysis, BrdU mark and S phase cell; the potential of multi-direction differentiation including immunocytochemical process and reverse transcriptase polymerase chain reaction.

由于神经干细胞的数量很少，从细胞形态来看神经干细胞为圆形或椭圆形，无或有较短的突起，核质比大，核染色较深，形态上与其它种类的细胞没有明显的差异，并且未找到一种细胞表面特异性标志物，因此目前鉴定神经干细胞主要有以下3个方面：特异性神经抗原的表达，包括巢蛋白、Musashi、转录因子及细胞黏附分子；自我更新能力，包括单细胞克隆分析、BrdU标记S期细胞；多向分化潜能，包括免疫细胞化学法、聚合酶链反应色法。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。