免疫化学

Methods:Via a cannula iserted into adominal aorta, Gey's balanced salt solution containing 0.025% collagenase P was perfused into rat normal pancreas. After digestion with collagenase P and protease, Nycodenz density gradient centrifugation was performed to isolate PSC. PSC was identified by its morphology, cytoplasmic lipid droplets and immunocytochemical staining for desmin, GFAP and α-SMA. Results:The production,viability and purity of isolated PSC were（15.3 ± 4.6）× 103／g body weight,（95.0 ± 3.5）%, and ﹥80％ respectively.

大鼠腹主动脉插管灌注后，经胶原酶消化、Nycodenz密度梯度离心，分离得到PSC；通过观察细胞形态、胞质内脂滴和免疫细胞化学方法检测结蛋白、神经胶质酸纤维蛋白（glial fibrillary acidic protein，GFAP）、α平滑肌肌动素（α-smooth muscle actin，α-SMA）的表达来鉴定PSC。

Methods: The distribution of T and B lymphocytes in gingiva were measured with histopathological and immunohistochemical methods in 15 elderly patients and 10 adult patients .

采用组织病理学和免疫组织化学方法，对15例老年牙周炎患者和10例成人牙周炎患者进行了T和B淋巴细胞分布情况的检查和比较。

The expression of MCP-1 in the glomerulus was determined by immunohistochemical staining.

采用免疫组织化学技术检测各组肾小球中MCP-1的表达。

Methods: The expression of glutamic acid receptor-2 in spinal cord was detected by immunohistochemical method and Western blot analysis.

应用免疫组织化学方法检测GluR-2在脊髓后角的表达，并用图像分析系统和Western印迹法进行定量处理。

Methods: Atrial tissue was obtained from the right appendage during open surgery in 25 patients with RHD. The mRNA of ACE and ERK2 and AT1R was semi-qualified by reverse transcription-ploymerase chain reaction and normalized to the glyceraldehyde 3-phosphate dehydrogenase. Western blotting analysis was employed to examine the expression of ACE and ERK and AT1R. Atrial collagen volume fraction was detected by Masson's stain.

25例风心病二尖瓣狭窄接受外科手术者，手术时取右心耳处心房组织500mg，通过逆转录—聚合酶链反应技术，以GAPDH为内参照测量ACE、AT1R、ERK2的 mRNA变化，用Western Blotting了解ACE、AT1R、ERK在蛋白水平表达的差异，经免疫组织化学了解胶原纤维容积分数改变情况。

Methods the immunohistochemical method was used to examine p -glycoprotein's expression in 64 samples of large intestine carcinoma.

应用免疫组织化学方法检测64例大肠癌标本的p-糖蛋白的表达。

Objective To explore the clinical significance of changes of serum IL-4 and IL-10 in patients with Graves' Disease.

目的：探讨IL - 4、IL - 10水平变化在甲亢的临床意义。方法：选择甲亢未治疗组6 4例、甲亢经1 31 I治疗组39例和正常对照组35例，用化学发光法测定FT3、FT4 、TSH的含量，用放射免疫分析测定IL - 4、IL - 10含量。

Methods Bioptic samples were taken from 8 cases of hypertrophic scar, 8 cases of superficial scar and 16 cases of controlled normal self-skin.MC-1R immunohistochemistry staining and its grey scale were examined in each sample.

利用兔抗人MC-1R抗体进行免疫组织化学染色，并测定8例增生性瘢痕，8例表浅性瘢痕和16例同体的正常皮肤标本切片染色后的平均灰度值，分别检测MC-1R在上述不同组织中表达的差异。

MethodsBioptic samples were taken from 8 cases of hypertrophic scar, 8 cases of superficial scar and 16 cases of controlled normal self-skin.MC-1R immunohistochemistry staining and its grey scale were examined in each sample.

利用兔抗人MC-1R抗体进行免疫组织化学染色，并测定8例增生性瘢痕，8例表浅性瘢痕和16例同体的正常皮肤标本切片染色后的平均灰度值，分别检测MC-1R在上述不同组织中表达的差异。

The location and dynamics of HSP70 expression in rat hepar was studied with immunohistochemistry, which were treated under different temperature (42、44、46℃) for 30min and different recovery time (2.5～72hr).

用免疫组织化学方法研究了大白鼠肝脏在46℃热休克30min后恢复期（2.5～72hr）内HSP70的表达定位和动态变化及不同温度（42、44和46℃）处理30min、恢复12hr后HSP70的表达。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。