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免疫化学

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Antibody of mouse against rat of glial fibrilla acidic protein used to labeling astrocytes of the brain and antibody of rabbit against rat of c-fos labeling c-fos protein in the brain. The second fluorescence antibodies were FITC and Cy3, respectively. Observations of the results and quantitative analysis were conducted with LSCM.

在本实验中,我们应用免疫组织化学的双标方法,用小鼠抗大鼠胶质原纤维酸性蛋白标记大鼠脑内的星形胶质细胞,用兔抗大鼠c-fos标记大鼠脑内的c-fos蛋白,分别应用FITC和Cy3标记的荧光二抗来显示,最后用激光扫描共聚焦显微镜进行观察并定量分析。

This study was conducted to determine the expression of the basic fibroblast growth factor in degenerated intervertebral discs.

本实验的目的是应用免疫组织化学和原位杂交的方法检测bFGF在退变的椎间盘组织中的表达。

Methods The expression of ER and PR in ectopic and eutopic endometrium were detected by immunohistochemistry SP method in 45 patients with endometriosis and in 32 patients with uterine fibroid.

采用免疫组织化学 SP法分别检测 4 5例 EM的异位和在位内膜及 32例子宫肌瘤的在位内膜中 ER、PR的表达。

Immunohistological method is applied to study the alteration of Fn expression in tumor tissue samples.

采用免疫组织化学方法观察胃癌组织内纤维连接蛋白表达的改变情况。

Set rats fornix transection models. The changes of expression and concentration of AVP in PVN of rats were detected by immunohistochemistry and analysis of images in each group.

建立大鼠穹窿切断模型,采用免疫组织化学和图像分析技术检测各组下丘脑PVN内AVP的表达变化和分布规律。

Methods Immunohistochemical method with two specific antibodies(MMP-9,CD68) was used to detect the their special antigens in lung tissues of six cases who died of SARS and five normal cases as the controls.Gomori aldehyde fuchsine staining method was used to explore the change of elastic fiber.Motic advanced 4.0 image analysis system was used to analyze those images.

方法通过免疫组织化学技术对6例SARS死亡患者肺和5例肺外伤患者手术切除的正常肺组织中MMP-9和CD68+细胞进行检测,Gomori醛品红法显示弹性纤维,应用Motic Advanced 4.0图像分析系统进行定量分析,测定MMP-9的面密度、CD68+细胞的数量、弹性纤维的含量,并与正常对照组进行比较。

Methods Keratinocytes monolayers and Malassezia furfur were co-cultured in vitro, and the modulating effects of Malassezia furfur on the expression of TLR-2 was measured by immuocytochemistry methods, the expression and secretion of sICAM-1 and IL-8 in keratinocytes was detected by enzyme linked immunosorbent assay.

马拉色菌和角质形成细胞体外共培养,通过免疫细胞化学方法检测角质形成细胞TLR-2蛋白的表达状况,并运用ELISA方法检测马拉色菌对角质形成细胞sICAM-1和IL-8表达和分泌的调节作用。

In order to study the regulatory mechanism in the gametogenesis of Acrida cinerea, the special expression of c-kit is investigated by immunohistochemical method.

本文首次采用免疫组织化学方法,研究了原癌基因c-kit在中华蚱蜢配子发生中的特异表达。

Methods: 76 cases with intracranical germ cell tumors were studies by immunohistochemical method with five different monoclonal antibodies.

对76例颅内生殖细胞肿瘤进行5种抗体的免疫组织化学染色。

Objective: To evaluate the value of immunohistochemical stain in diagnosis for various intracranial germ cell tumors.

目的:探讨各种颅内生殖细胞肿瘤中免疫组织化学染色的诊断意义。

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