免疫化学

Isolation, cultivation and identification of human embryonic NSC from cerebral cortex of 7-12 week abortus. By using flow cytometry and MTT assay, the effects of various concentration of TSPG and TSPG cooperating with cytokines in NSC culture media for 3 days on proliferation of human embryonic NSC has studied. By employing immunocytochemistry assay of the expression of tyrosine hydroxylase, the effect of different dilution of TSPG and TSPG cooperating with IL-1 on induced differentiation of human embryonic NSC into dopaminergic neuron has researched.

从7～12周人工流产胚胎脑组织中分离培养人胚胎NSC，免疫细胞化学鉴定NSC特异性nestin抗原表达，在NSC的培养体系中加入TSPG，采用流式细胞术和MTT法检测不同浓度TSPG以及TSPG与EGF，bFGF联合应用对人胚胎NSC增殖的影响；经免疫细胞化学检测培养细胞酪氨酸羟化酶表达，分析不同浓度TSPG以及TSPG与IL-1联合应用对NSC定向诱导为DA能神经元的影响。

Objective:To assess the role of carcinoembryonic antigen immunocytochemistry in the diagnosis of meningeal carcinomatosis.

目的 ：探讨癌胚抗原免疫细胞化学方法在诊断脑膜癌病(meningealcarcinomatosis，MC)中的作用。方法：对1 3例确诊为MC患者的 2 1份脑脊液标本、5例非MC患者的 7份标本分别进行常规脑脊液细胞学检查和CEA免疫细胞化学检查。

Methods: Histochemistry, immunohistochemistry, histochemistry combined with immunohistochemistry technique and morphometric analysis were used in this study.

应用组织化学、免疫组化、组织化学与免疫组化结合的方法和形态计量学方法进行观测。

RESULTS: A total of 36 rabbit models, 6 in the 1-month therapy group and 8 in the 3-month therapy group were died from infection. In the 1-month therapy group, thick ureteric wall and smooth ureteric lumina developed without any contracture. Under the optical microscope, transitional epithelial cells covered throughout the inner walls and smooth muscle cells appeared sparsely in a mussy configuration. In addition, CKAE1/AE3 staining was positive butα-actin staining was very weak when checked by immunohistochemistry. In the 3-month therapy group, amnion-ureters were enclosed by their peripheral tissue and showed rich blood vessels and normal ureteric lumina without significant contracture, futher more, there had formed a well-arranged transitional epithelial layer and smooth muscular layer, the immunohistochemistry result showed that the expression of CKAE1/AE3 and α-actin were positive.

结果：36只大白兔中，1个月治疗组死亡6只，3个月治疗组死亡8只，死亡原因均为感染。1个月治疗组大白兔羊膜输尿管柔软，长4 cm，管壁变厚，无挛缩，移行上皮组织爬满内壁，平滑肌组织部分再生，但较稀疏，排列不规则；免疫组织化学检查提示CKAE1/AE3染色阳性，α-Actin染色微弱。3个月治疗组大白兔羊膜输尿管与周围粘连，有丰富的血运，无明显挛缩，管腔不缩小，已形成良好的移行上皮层和平滑肌层，免疫组织化学显示α-Actin和CKAE1/AE3染色均为阳性。

Methods: The expression of VEGF by immunohistochemical staining was examined in 61 cases of the esophageal carcinomas and 13 cases of normal esophageal mucosas. Microvessel density in the cases was determined by FactorⅧ immunohistochemical staining.

应用免疫组织化学方法检测61例食管癌组织及13例正常食管粘膜组织的VEGF表达，并对血管进行Ⅷ因子相关抗原的免疫组织化学染色，计数微血管密度。

Results Trunk neural crest stem cells were successfully isolated and cultured. The immuocytochemcal result showed that the cells were nestin-and P75-positive. Trunk neural crest stem cells grew through adhering to chitosan fiber after inoculation. Under an electon microscope, the spindle-shaped trunk neural crest stem cells were proliferated and migrated along the fiber. The cells arranged side by side or linked by heads and tails in processes. The ends of S-100 positive cells were flat and expanded, adhering to the fiber as claw-shaped pseudopodium.

结果 采用神经管植块法培养的神经嵴干细胞，免疫细胞化学染色呈现nestin及P75双阳性，将其接种在壳聚糖纤维支架材料上，可见神经嵴干细胞贴附于壳聚糖纤维上生长；S-100免疫细胞化学染色显示，S-100阳性细胞突起的末端呈扁平状膨大；扫描电镜显示，壳聚糖纤维上的躯干神经嵴干细胞为梭形，呈现"端对端"、"肩并肩"排列，伸出爪形伪足贴附于壳聚糖纤维上。

A series of indexes reflecting free radical changes, anti-oxidations, Immune function, endocrine function was studied by Chemiluminescence method, immunoturbidimetry, ANAE dye method, micro-hemagglutination inhibition test and radio immunoassay in the chicken with encephalomalicia induced by giving the feed being deficient in VE and extra fish oil artificially. The pathological changes including microscopic and ultramicroscopic changes in the relative organs and tissues were observed.

本实验以一日龄150只海兰兰蛋鸡为实验动物，用低维生素E且添加鱼油日粮饲喂雏鸡，复制脑软化症动物模型，采用化学发光法、免疫浊度测定法、酸性α-醋酸萘脂酶染色法、微量血凝抑制试验、放射免疫测定技术等方法系统地检测了脑软化雏鸡的自由基代谢和抗氧化功能、红细胞免疫功能、细胞免疫功能、体液免疫功能及非特异性免疫功能等指标，并对患雏病理组织进行了显微及超微结构观察。

In this study, the expression and function of FGF-13 in developmental follicles and skin during embryonic development,normal vibrissa follilce stem cells and wound follicles in vitro were obserbed,so as to illuminate the possible role of FGF-13 in the skin follicles during embryonic development and damage healing and provide the experimental base for further studying the characteristic and mechanism of the role of FGF-13 in skin.

采用免疫组织化学(Immunohistochemistry，IHC)、免疫细胞化学(Immunocytochemistry， ICC)和原位杂交(in situ hybridization，ISH)技术及体外大鼠触须毛囊的器官培养，研究了三种不同的生物学过程中FGF-13在皮肤毛囊中的表达及作用。

objective the aim of this study is to investigate the expression and the distribution of the nerve growth factor during the period of neural tube development of human embryo.method early development of neural tube was studied in human embryos about 35 gestational days by using immunocytochemical abc technique.result there were ngf immuno-positive substances in the cytoplasm and nuclei of neuroepithelial cells in the ventricular zones of neural tube.in the intermediate zone of neural tube,ngf immunoreactivity was detected in the nuclei of some neurons,or the processes of other neurons which contained no ngf-immunoreactive substances in their nuclei;the expression pattern of ngf in the marginal zone of neural tube was similar to that of the intermediate zone.the density of ngf-immunorecative particles was higher on the rostrum side of neural tube than on the caudal side.the ngf immuno-positive cells were also observed among the somites of embryo under the neural tube.conclusion these results suggest that ngf was an important signal molecule to induce neural tube differentiation,and that ngf may play a significant role in regulation of the biological function of neurons in developing neural tube.

目的 研究捷安肽素的抗真菌作用机理。方法采用形态学方法和同位素标记法。显微形态观察经捷安肽素处理后的供试真菌的形态学变化。进一步采用14c同位素标记的特异底物&尿苷二磷酸-（14c）-葡萄糖&示踪，研究捷安肽素对真菌(1，3)-β-d-葡聚糖合成酶活性反应的影响。结果研究神经生长因子在早期人胚神经管发育过程中的定位表达。方法采用免疫细胞化学 abc法染色，研究35天人胚的发育情况。结果在人胚神经管的室管带中，神经元的细胞质和细胞核ngf免疫反应阳性；在中间带，一部分神经元的细胞核ngf免疫反应阳性，另外一部分神经元的细胞核ngf免疫反应阴性，而其突起ngf免疫反应阳性；在边缘带ngf的表达与中间带相似。在神经管的头侧ngf阳性反应较强，神经管的尾侧ngf阳性反应较弱。结论 ngf在人胚神经管免疫反应阳性，表明ngf可能是诱导神经管分化发育的重要信号分子，提示ngf可能在人胚神经管的发育中具有十分重要的作用。神经生长因子；人胚；神经管；发育

In our research,Wistar rats and SD rats which were parathyroidectomized were used as donors and recepients respectively.The parathyroid gland of the Wistar rat were allografted into the cerebroventricle and other different sites of the brains in SD rat s tereotaxically. Using methods of testing serum Ca~(2+) concentration,histological examination,histochemical and immunocytochemical methods and electron microscopic observation,we investigated the survival of the grafting tissue,its revascularization, infiltrating of monocytes, changes of microglia and astrocytes, the relationship of the CNS with the immune system, and the mechanism of rejection were investigated.The results as following: I .Effects on PTG after allografting into cerebroventricle and cerebral parenchyma of the brain: Testing serum Ca~(2+) conc.

本研究以Wistar大鼠作为供体，切除甲状旁腺的SD大鼠作为受体，在立体定位下将同种异体的PTG移植到大脑的不同部位，设计了侧脑室-内分泌系统移植的代替途径，比较了侧脑室内移植和脑实质内移植的异同，通过测定血清Ca~(2+)的浓度、组织学检查、组织化学和免疫细胞化学以及电镜观察对移植后PTG组织的存活、血管的再生、单核细胞的浸润、CNS内胶质细胞的变化、对免疫系统的影响及免疫排斥的机制进行了研究，结果如下：一、同种异体PTG侧脑室内移植与脑实质内移植的比较：血清Ca~(2+)的检查发现，PTG侧脑室内移植后第7天，血清Ca~(2+)的浓度恢复到8mg％以上，一直持续到移植后第8-12周；移植后第12周仍有60％移植PTG的动物血清Ca~(2+)维持在正常水平。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。