免疫化学

NF-κB expression was detected by immunofluorescence cytochemistry, and the viability of the PC12 cells was demonstrated by MTT assay.

免疫荧光细胞化学法测定软骨藻酸对PC12细胞NF-κB蛋白的影响；MTT法检测PC12细胞的存活率。

The result of immuno-electron cytochemistry showed the number of positive reaction cells of gastrin increased significantly.

胃泌素免疫电镜细胞化学观察结果显示阳性细胞数量明显升高。

Real-time PCR and immunofluorescein cytochemistry were performed to analyze the expression of RAGE after 2 days' stimulation of IL-1β.

IL-1β刺激2天后使用实时PCR和免疫荧光细胞化学分析RAGE受体的表达。

Third, the prepared McAbs were identified by cytochemistry, specificity and Western-blot.

然后用细胞化学、特异性、Western-blot等方法鉴定制备抗体的免疫反应及特异性。

The aim of this study was to establish the culture system of isolation and cultivation of the neural stem cells from the embryonic rat brain and spinal cord. The methods of microscopic dissection, cell culture and immunofluorescence cytochemistry were used.

研究采用显微解剖、无血清细胞培养和免疫荧光细胞化学染色等实验技术，成功地建立了胚胎大鼠脑和脊髓神经干细胞的分离和培养方法。

The aim of this study was to establish the culture system of isolation and cultiation of the neural stem cells from the embryonic rat brain and spinal cord. The methods of microscopic dissection, cell culture and immunofluorescence cytochemistry were used.

研究采用显微解剖、无血清细胞培养和免疫荧光细胞化学染色等实验技术，成功地建立了胚胎大鼠脑和脊髓神经干细胞的分离和培养方法。

METHODS: The immunocytochemistry method was used to as say the expression of HnRNP A2/B1 in fiberobronchoscopic aspirates of lung cancer confirmed by histopathology and/or cytopathology and of benign lung diseases.

利用免疫细胞化学的方法检测经病理学或细胞学确诊的肺癌患者和非肺癌患者的纤支镜抽吸物中HnRNP A2/B1的表达情况，并分析其表达水平的差异性。

Methods: Introduce cytopathology experimental methods, human hepatic cancer cell line Bel-7402 co-cultured with Triptolide (10 g/mL, 5μg/mL and 0.25μg/mL), Using MTT method to test the effect of Triptolide on Bel-7402 cell proliferation, using TUNEL technique to test the effect of Triptolide on Bell-7402 cell apoptosis, immunohistochemistry staining method to test Bax and Bcl-2 protein expression.

采用细胞药理学实验方法，人肝癌细胞株Bel-7402与雷公藤甲素（10μg/mL、5μg/mL及0.25μg/mL）共培养，MTT法检测其对Bel-7402细胞增殖的影响，TUNEL技术检测其对Bel-7402细胞凋亡的影响，免疫组织化学染色法检测Bax及Bcl-2蛋白表达。

The positive expression of Slit2 located in the cytoplast on oligodendrocyte and astrocyte.The positive cells were found at 3d after spinal cord injury, reached peak on 7d after injury, declining after 14d. The change of Slit2 was correlative with the rehabilitation and regeneration of the axon on the forepart period.

免疫组织化学检测：Slit2蛋白定位于星型胶质细胞和少突胶质细胞的胞浆，呈棕黄色染色，于脊髓损伤后第3 d出现，阳性细胞逐渐增多，于脊髓损伤后第7 d，Slit2阳性表达的细胞数达到高峰并趋于稳定，伤后第14 d开始逐渐下降，呈一过性增高。

Methods: Expression levels of MMP10 gene and protein in human spontaneous abortion decidua were determined by RT-PCR, Western blotting and distribution by immunohistochemistry.

利用RT-PCR和Western blotting技术分别检测MMP10基因和蛋白在人正常蜕膜和自然流产蜕膜的表达水平、利用免疫组织化学技术检测MMP10在人正常蜕膜和自然流产蜕膜组织中的分布。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。