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免疫化学

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Results Primary RPCs extracted from the neural retina demonstrate certain biological characteristics: the cell population could form neurospheres and younger adherent cells expanded in clusters.

结果 从兔胚胎视网膜神经部分离出的原代视网膜前体细胞具有明显的生物学特性:所培养细胞可形成悬浮生长的细胞团,刚贴壁的细胞成簇状生长,这些细胞用免疫细胞化学染色神经上皮干细胞蛋白表达呈阳性。

The principle fields of the Institute's activity are related to molecular genetics, cytogenetics, immunogenetics, embryology, biochemistry, the physiology of animal stress and ethology, embryo biotechnology and biotechnics, introductions of the results of basic investigations into animal improvement programmes, determination of animal genotypes and their adaptation to environmental conditions and production demands.

该所的主要活动领域是关于分子遗传学、细胞遗传学、免疫遗传学、胚胎学、生物化学、动物胁迫和动物行为方面的生理学、胚胎生物技术和应用生物学、改善动物的项目基础研究结果的介绍、动物基因型的决定及其对环境条件的适应与产品要求等方面的内容。

Using immuno histochemical analysis was seen a positive staining in the basal cytoplasm of blastoderm embryos at 4 hours. This result confirms that the DLMO is a cytoplasmprotein, not like human LMO protein.

免疫组织化学的分析在0~4小时胚盘外周胞浆中有Dlmo基因的阳性染色信号,结果证实,Dlmo与人类LMO不同,其表达产物是一个胞浆蛋白。

He proteins of TLR2, 3, 4 and 9 were also comparedby immunofluorescence staining and western blot.

LR2,3,4,9蛋白水平的表达使用荧光免疫组织化学和western blot来检测。

Using the immunohistochemical technique, nerve cell bodies and fibres immunoreactive to the anti-Met-enkephalin sera were observed in the cerebral ganglia, pedal ganglia, visceral ganglia, inspect ganglia and coat ganglia of Octopus ocellatus. Met-ENK immunoreactivity was also localized in the mouth, esophagus, craw, stomach, stomachic caecus, the epithelium of the intestines, rectum, sialoid gland, liver, branchia, branchia gland, ventricle, branchial heart, kidneys, ovary, fallopian tube, back of mantle, abdomen of mantle, wrist, membrane of wrist, funnel, and immunocytes of Octopus ocellatus.

免疫组织化学定位结果表明:(1)在短蛸的脑神经节、足神经节、脏神经节、视神经节、外套神经节、口球、食道、嗉囊、胃、胃盲囊、肠、直肠、前唾液腺、后唾液腺、肝胰脏、鳃、鳃腺、心室、鳃心、肾静脉腺质附属物、卵巢、输卵管腺、外套膜背面、外套膜腹面、腕、腕间膜、漏斗中检测到呈不同阳性反应的Met-ENK分子。

By immunohistochemistry, porcine parvovirus signals were mainly distributed in interstitial and necrotic lesions, and the positive signals could be found in macrophages-like cells and epithelial cells of terminal bronchiole and alveoli.

另外,以免疫组织化学染色检测猪小病毒,其阳性讯号主要分布於间质及坏死病灶区,此阳性讯号存於巨噬细胞与肺泡及终末细支气管上皮细胞。

The auditory thresholds were examined through the auditory brainstem responses to click, which tone pips were 8, 16, and 32 kHz. The mice were intracardically perfused with 4% paraformaldehyde. The bulla were further fixed in 4% paraformaldehyde, processed and sectioned with paraffin embedded method.

用听性脑干反应测试短声、短音8,16和32 kHz听阈,经活体心脏灌注固定、听泡石蜡包埋和耳蜗连续切片,用免疫组织化学方法确定上述3种酶在内耳的分布情况。

METHODS: Autologous whole blood was injected into the vitreous cavity of albino rabbits after canalization, thus induced experimental PVR model. Concentration of PDGF.

采用眼穿通伤+注血法制备PVR模型,进行眼底及常规组织病理学观察,ELISA法检测玻璃体中血小板源性生长因子的浓度,用免疫组织化学方法观察增生膜与血管内皮细胞表面血管细胞粘附分子-1(VCAM-1)及细胞间粘附分子-1(ICAM-1)的表达。

Abstract] Objective To investigated the cellular distribution and quantitatively of CEA in cerebrospinal fluid meningeal carcinomatosis cells by immunofluorescence staining and laser scanning confocal microscopy, explore the diagnostic value of the examination in the patients with meningeal carcinomatosis.

目的 联合应用免疫荧光细胞化学技术和激光扫描共聚焦显微镜技术检测脑脊液中脑膜癌细胞CEA的表达部位并定量测定其荧光含量,探讨该技术在脑膜癌病诊断中的价值。

Methods:Twenty one cerebrospinal fluid specimens from 13 patients with meningeal carcinomatosis and 7 specimens from 5 non meningeal carcinomatosis patients were tested with routine cytologic examination and CEA immunocytochemistry examination.

结果 :常规脑脊液细胞学检查阳性率 4 2 。86 %(9/ 2 1 ),CEA免疫细胞化学检查阳性率 80 。95 %(1 7/ 2 1 ),两者相差显著(P 。0 5 );其中对首次腰穿获得的脑脊液标本检查,两种方法的阳性率分别为 30 。77%(4 / 1 3)和 76 。92 %(1 0 / 1 3),两者相差也显著(P 。0 5 );非脑膜癌病患者的脑脊液检查结果均为阴性。

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