免疫化学

METHODS: The expression of MPF was investigated in 40 salivary adenoid cystic carcinomas and 40 normal salivary tissues by immunohistochemistry.

应用免疫组织化学SP法检测40例SACC组织及40例正常唾液腺组织中MPF的表达。

Methods:uPA was deteced by immunohistochemistry in 109 cases with breast cancer and 23 cases with adenoma.

采集乳腺腺瘤23例，乳腺癌109例，利用免疫组织化学方法检测uPA的表达情况。

Methods: fifteen sd rats were randomly divided into five groups (n=3), and adrenalectomy was performed and anterior pituitary specimens were obtained after a week. anti gap 43 antibody and anti adrenocorticotrophic hormone, thyroid stimulating hormone, luteinizing hormone, prolactin and growth hormone antibodies were used in the double immunohistochemical staining.

将成年雄性sd大鼠15只随机分为5组(n=3)，手术切除大鼠双侧肾上腺，1 wk后取垂体前叶标本分别进行抗gap 43与抗促肾上腺皮质激素、促甲状腺激素、黄体生成素、催乳素、生长激素抗体的免疫组织化学双标染色，观察gap 43 li神经纤维与各腺细胞之间的关系。

RESULTS: A great deal of neurospheres were formed in the presence of the extracts of afterbrain and cerebellum, which were positive for characteristic protein of NSC showed by immunocytochemical staining.

结果： 中脑和小脑的提取液加入后有大量神经球生成，神经干细胞的特异性蛋白nestin免疫细胞化学染色呈阳性。

METHODS: The extracts of forebrain, midbrain,afterbrain and cerebellum of neonatal mice were prepared, and the NSCs of newborn mice were cultured in vitro. Neurospheres were observed, immunocytochemical staining of characteristic protein, nestin, and MTT assay were performed to identify NSCs and their proliferative properties.

制备新生鼠的前脑、中脑、后脑及小脑组织提取液，体外培养新生鼠神经干细胞，观察神经球的生长情况，采用MTT细胞活性检测法和特异性蛋白质免疫细胞化学染色鉴定神经干细胞的增殖能力。

Rt-PA(5mg/kg) dissolve bolt gives treatment after the 3h that be short of blood, associated group gives vein injection at the same time Yidala is acted according to (the 24h after 3mg/kg) dissolve bolt undertakes nerve function grading and cerebral stalk die concentration percentage by volume leads an analysis, determine blue content of the thread of ideas in writing of the Yi in cerebral tissue, with immune organization chemical method evaluates dissolve bolt afterbrain to organize the change of hematic head protective screen.

缺血3h后给予rt-PA(5mg/kg)溶栓治疗，联合组同时给予静脉注射依达拉奉(3mg/kg)溶栓后24h进行神经功能评分和脑梗死体积百分率分析，测定脑组织中伊文思蓝含量，用免疫组织化学方法评估溶栓后脑组织血脑屏障的变化。

Rat in Group B was set an air bladder which was empty and not perfused water. Water of 0.6 ml, 0.8 ml, 1.0 ml, 1.2 ml, 1.4 ml and 1.5 ml was perfused respectively into the air bladders which were set in the colon of rats in group C, D, E, F, G and H. Then the changes of ethology of the rats were recorded in interval of an hour.

应用疼痛行为学的观察方法，观察1h各组动物的行为学改变，并采用免疫荧光化学的方法，观察大鼠DCN中神经元被激活的标志c-fos及胶质细胞的胶质原纤维酸性蛋白(glial fibrillary acidic protein， GFAP)和小胶质细胞特异性补体OX42的表达情况，空白对照组是在疼痛高峰点(1.2 ml)处死取材。

The content of soluble protein rapidly increased with the embryo development after pollination for 16 days, while BiP markedly increased from 22nd day after pollination. The BiP failed to respond to heat shock. Immunohistochemical localization showed that BiP localized in plumule, primary vascular tissue and aleurone layer during embryo development.

组织化学免疫定位表明，在玉米胚发育的不同时期，BiP主要定位在胚芽端、初生维管组织和糊粉层中，提示胚在构建器官的同时，也为其功能执行准备了条件；热激不影响其定位。

METHODS: Adult male Wistar rats, 9 weeks of age, were injected intraperitoneally with STZ to create diabetes. At 2 week after the models were created, BDNF protein was administered into the vitreous cavities of rats. At 4 week after the models were created, the rats were killed and the retina was removed for Western blotting and Whole-mount immunohistochemistry for TH to observe the changes of TH and dopaminergic amacrine cells in retina.

9周龄Wistar大鼠，链脲佐菌素（streptozotocin，STZ）腹腔注射，制成糖尿病模型，于模型建立2wk后，大鼠眼玻璃体内注射BDNF溶液，于模型建立4wk后，处死大鼠，取出眼球，取视网膜组织进行Western blotting及视网膜铺片免疫组织化学染色检测，观察视网膜中TH水平的变化，从而反映糖尿病大鼠视网膜中多巴胺能无长突细胞水平的变化。

This finding corresponded to the immunohistochemical data showing the presence of phosphorylated forms of p38, c-Jun N-terminal kinase, and extracellular signal-regulated kinase during ameloblast differentiation.

这个发现与免疫组织化学数据相符，显示在成釉细胞的分化过程中，p38磷酸化形式，c-Jun氨基端激酶以及细胞外信号调节激酶均有表达。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。