免疫化学

The dynamic changes of the number of T or B lymphocytes and the content of immunoglobulin G, IgM, IgA in peripheral blood, the number of CD〓, CD〓 lymphocyte subpopulation, T〓 cells and IgG, IgM, IgA antibody-producing cells, the ratios of CD〓 and CD〓 T cells in immune organs (thymus, bursa of Fabricius and spleen) and immune tissues (cecal tonsils and Harderian glands), the content of IgA, IgM, IgG in local fluid (tear, tracheal fluid, intestinal fluid and bile) of one-day chicks infected with chicken infectious anemia virus and CIAV-infected chicks immunized with Newcastle disease vaccine were determined respectively by immunohistochemistry, immunogold-silves and immunoenzyme staining in order to reveal mechanism of immunopathogenesis of chicks infected with CIAV and influence of immune responses of CIAV-infected chicks to ND vaccine.

中文题名雏鸡感染鸡传染性贫血病毒后免疫变化和对新城疫疫苗的应答及其免疫机理副题名外文题名 Immune changes of chicks infected with chicken infectious anemia virus and immune response and mechanism to Newcastle disease vaccine immunity 论文作者郑世民导师刘忠贵教授学科专业基础兽医学研究领域\研究方向学位级别博士学位授予单位东北农业大学学位授予日期2002 论文页码总数105页关键词鸡传染性贫血病雏鸡新城疫疫苗馆藏号BSLW /2003 /S858 /9 本项研究采用免疫组织化学、免疫胶体金及免疫酶方法，首次对CIAV感染1日龄雏鸡和CIAV感染雏鸡ND疫苗免疫及vNDV攻击后，其外周血液的T细胞、B细胞数量和IgG、IgM、IgA含量；中枢免疫器官胸腺和法氏囊，外周免疫器官脾脏及呼吸道和消化道局部粘膜免疫组织哈德尔腺和盲肠扁桃体的ANAE〓、CD4〓、CD〓T细胞、IgG、IgM、IgA抗体生成细胞数量和CD〓与CD〓T细胞比值以及泪液、气管液、肠液、胆汁的IgA、IgM、IgG含量的动态变化规律和CIAV感染对ND疫苗的免疫保护率等进行了全面系统地研究。

Using neuroanatomical tracing technique and immunohistochemical staining methods, we studied whether transplantation of adult bone marrow stromal cells could promote axon regeneration after spinal injury.

本篇文章利用神经解剖追踪及免疫化学染色的方法探讨脊髓损伤后移植骨髓基质细胞是否帮助脊髓再生。

No analogues of nitroaniline was found to interfere with the analysis of nitroaniline.

成功地建立了硝基苯胺类化合物的间接竞争酶免疫化学分析方法。

In this study, the ovarial cancer stem cells were successfully obtained through the suspension culture of the ovarian cancer 0VCAR3 cell line in serum-free media. The stem cells formed spherical colonies, called "ovarispheres", and showed anchorage-independent ability. Furthermore, the bionomics and morphology of the stem cells were observed, and the expression difference of the tumor stem cells marker-ABCG2 between ovarispheres and anchorage-dependent cells was identified by using RT-PCR and Immunocytochemistry methods.

本研究通过无血清悬浮培养人卵巢癌细胞系OVCAR3，获得具有非锚着依赖能力的肿瘤干细胞球，观察其生物学特性及形态学的变化，并采用半定量逆转录—聚合酶链反应及细胞免疫化学的方法鉴定肿瘤干细胞标志ABCG2在贴壁细胞及肿瘤干细胞球中的表达差异。

In the second experiment, we would like to understand the relationship between sexual performance and sexual motivation. The partner preference test was conducted to measure sexual incentive motivation, and tyrosine hydroxylase immunohistochemistry was used to assay the dopaminergic neuron activity in the medial preoptic area.

在第二部份实验所探讨的是雄鼠的性动机与性表现两者之关系，本实验以EGb 761作为一实验工具，利用伙伴偏好试验测试雄鼠性诱因动机，并利用酪氨酸羟化酶(tyrosine hydroxylase， TH)免疫化学染色方法分析内侧视前区多巴胺神经元的活性。

①Rat MSC and VSMC were cultured and identified, respectively. MSC were labeled with DAPI firstly, and then co-cultivated with VSMC. The changes of morphology and ultrastructure of co-cultured cells were observed. Immunfluorescence analysis was performed by using monoclonal antibodies against specific antigen.②We established the regulatable system in two steps: a stable MSC line expressing rtTA has been constructed and characterized firstly by transfected with pUHD 17-1hyg and then selected by hygromycin B; in a second step, this line was used for trandfer the AT2R gene to MSC to get the well establishing double stable MSC lines;③The expression of AT2R regulated by doxycycline was evaluated by western blot;④The MSCs were transduced into rat carotid arteries with regulatable AT2R gene after the establishment of rat carotid balloon injury restenosis model. The intimal/medial area ratio were measured by digital analysis system.

研究方法：(1)密度梯度离心法及胶原酶消化法分别培养原代大鼠MSC及VSMC，细胞共培养并行免疫荧光化学染色和透射电镜观察超微结构；(2)组成受Dox调控的哺乳动物表达系统的四种成分的转化、扩增及提纯并酶切鉴定；(3)采用常规分子生物学方法连续两个回合转染体外培养的MSC，并分别采用发光计检测不同细胞克隆萤光素酶活性改变以及RT-PCR方法检测AT2R目的基因mRNA表达情况，根据各个细胞克隆受Dox调控表达的程度，选择低背景、高诱导表达AT2R的细胞系，作为双重稳定MSC细胞系；蛋白免疫印迹法观察该细胞系在Dox调控下AT2R表达的时相性、持续性及在不同浓度Dox调控下的表达情况；(4)建立大鼠颈动脉球囊损伤动物模型，将双重稳定MSC在术中导入血管，分别于14 d、28 d进行病理切片，检测可调控AT2R对新生内膜增生的影响；采用RT-PCR免疫组织化学免疫荧光等技术观察AT2R基因在新生内膜中的表达以及细胞外基质成分表达的改变，TUNEL法检测血管组织中细胞凋亡的变化情况。

Desmin, the marker of myoblasts, was adopted to identify the expression of specific marker protein of sarcoblast desmin with immunohistochemistry. Desmin negative cell clones were removed, and desmin cell clones were cultured continuously with the culture fluid replaced once every other day.

采用成肌细胞特异性标志抗原desmin免疫化学染色，鉴定成肌细胞标志蛋白——结蛋白的表达，弃去desmin阴性的细胞克隆，继续培养desmin阳性的细胞克隆，隔天换液1次，7 d进行酶消化传代，获得大量扩增的细胞，并可冻存复苏，用于实验。

Subjects who had taking iodine-containing preparations or thyroid hormone were also excluded.2.2 MethodsSerum levels of triiodothyronine ( T3 ), thyroxine ( T4 ), free triiodothyronine ( FT3 ), free thyroxine ( FT4 ) and thyrotropin were determined by immunochemiluminometric assay for ninety-fifth patients with atrial fibrillation and one hundred and thirty nine controls matched for age and sex.2.3 StatisticsAll statistical procedures were performed with SPSS 10.0 software package.

同时根据甲状腺功能指标的测定把心房颤动患者分为①正常组：TSH、FT3和 FT4水平均正常②甲状腺功能亢进组：TSH水平降低，FT3和 FT4水平升高③亚临床甲状腺功能亢进组：TSH水平降低，FT3和 FT4正常。2.2方法入选者于清晨空腹静脉取血 2 ml，采用免疫化学发光法分别测定血清中的三碘甲状腺原氨酸（T3）、甲状腺激素（T4）、游离三碘甲状腺原氨酸（FT3）。

By whole mount in situ hybridization and immunohistochemical staining, we also found that the p73 was expressed in the olfactory bulb—like region and in specific cell types of the reproductive organs.

此外，利用全体原位杂交及免疫化学染色方法，发现p73也在头部的嗅球及生殖器官的特定细胞上有表现。

Mortalities of IP and IM group were 100% and 50% after 6 days and 10 days, respectively.

免疫化学染色於坏死及肉芽肿病灶区为阳性反应。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。