免疫化学

Methods: Integrated optical density was measured by ABC immuno-histochemical and in situ hybridization technique with computerized image processing. The rat′s brain was coronally sectioned after combination of EA and MEL.

采用ABC免疫组织化学和原位杂交组织化学技术，结合计算机图像处理，测定其积分光密度值。

However, the molecular mechanisms underlying premature aging up 8-MOP/UVA are as yet unknown. In our studies, dermis of the non-lesion back skin of vulgar psoriatic patients who had been treated by PUVA and the cultured foetus dermic fibroblasts were selected to be observed.

本课题以PUVA治疗寻常型银屑病患者背部非皮损区真皮及培养的胎儿真皮成纤维细胞为对象，采用HE染色、Verhoeff染色、电镜、酶组织化学、免疫组织化学、原位杂交、流式细胞计数仪、三引物PCR、PCR-SSCP技术及PCR产物直接测序等技术和方法，研究了8-MOP/UVA对皮肤光老化形成的影响及芳维A酸乙酯的拮抗作用。

Use NADPH-d to organize chemistry and ABC immunity systematism to learn a method, observe leaf of different dosage ginkgo extracts content gawkish to hemal sex small rat pallium NOS and NNOS positive nerve yuan quantitative influence.

采用NADPH-d组织化学和ABC免疫组织化学方法，观察不同剂量银杏叶提取物对血管性痴呆小鼠大脑皮层NOS和nNOS阳性神经元数量的影响。

In this research, we made use of classical histochemical methods and simple immunohistochemical methods.

本论文完全利用传统的组织化学染色和简单的免疫组织化学染色来进行研究及评估。

Samples were extracted 6 and 12 weeks after operation for histological, histochemical and immunohistochemical analysis.

术后 6周和 12周分别处死动物、取材，进行缺损区大体、组织学、组织化学和免疫组织化学分析。

Based on relative literatures,this part has included five resesearch aspects as below.1 Fifteen compounds were isolated and purified by extraction,column chromatography,and their structures were determined on the basis of spectral analysis: acteoside(Ⅰ-1),isoacteoside(Ⅰ-2),crenatoside(Ⅰ-3),cistanoside F(Ⅰ-4),sinapoyl-4-O-β-D-glucoside(Ⅰ-5),adenosine(Ⅰ-6),β-siterol(Ⅰ-7),oleanic acid(Ⅰ-8), succinic acid(Ⅰ-9),caffeic acid(Ⅰ-10),protocatechuic aldehyde(Ⅰ-11),p-hydro xybenzyl alcohol(Ⅰ-12),β-daucosterol(Ⅰ-13),D-galacitol(Ⅰ-14),D-mannitol(Ⅰ-15).Ⅰ-4～15 were obtained from this plant for the first time,andⅠ-6,7,9,and 13 were isolated from Orobanche genus for the first time.2 The scavenging test of DPPH showed that most compounds have comparative antioxidant activity as L-ascorbic acid and part of them show better activity such as the O.coerulescens extract and phenylethanoid glycosides.Acteoside showed potent free radical scavenging effects with a median inhibition concentration of 25.6μg/ml.3 The anti-HBV activities of acteoside,isoacteoside and crenatoside were measured,and all of them showed suppressive activity on the expression of HBsAg and HBeAg in the HepG2.2.15 cell line.

本论文在文献调研基础上对紫花列当化学成分及生物活性进行了研究，并从免疫抗病毒角度探讨紫花列当中特征性成分类叶升麻苷的肝保护作用及其机制。1采用大孔树脂、硅胶和Sephadex LH-20等色谱技术对紫花列当进行系统的植物化学研究，从中分离得到19个化合物，利用UV和NMR等波谱手段及理化性质鉴定了其中的15个化合物，分别为类叶升麻苷(Ⅰ-1)、异类叶升麻苷(Ⅰ-2)、crenatoside(Ⅰ-3)、cistanoside F(Ⅰ-4)、sinapoyl-4-O-β-D-glucoside(Ⅰ-5)、腺苷(Ⅰ-6)、β-谷甾醇(Ⅰ-7)、齐墩果酸(Ⅰ-8)、琥珀酸(Ⅰ-9)、咖啡酸(Ⅰ-10)、原儿茶醛(Ⅰ-11)、对羟甲基苯甲酸(Ⅰ-12)、β-胡萝卜苷(Ⅰ-13)、D-半乳糖醇(Ⅰ-14)和甘露醇(Ⅰ-15除化合物Ⅰ-1、Ⅰ-2和Ⅰ-3外，其余化合物均为首次从该植物中分离得到。2)通过大孔树脂富集该药材有效部位苯乙醇总苷，并采用HPLC测得其所含特征性成分类叶升麻苷的含量可达80%以上。3DPPH自由基清除试验显示紫花列当提取物及其所含的苯乙醇苷类化合物均具有较好的抗氧化能力，其清除DPPH自由基能力接近于抗坏血酸。4采用卡介苗整体致敏、脂多糖离体攻击构建大鼠原代肝细胞免疫性损伤模型，在体外观察类叶升麻苷的保肝作用。

To observe the expression of Bcl-2 and Bax in rat normal and inflammatory dental pulp in situ, and to explore the impact of Bcl-2 and Bax on pulpitis.

利用 SD大鼠的下颌第一磨牙采用内毒素化学诱导法建立牙髓炎模型，用免疫组织化学染色的方法研究Bcl-2和Bax的表达及其意义。

Methods We reported a case of PXA located in the cerebellum of a young adult in view of its extremely unusual location. Morphologic observation, immunohistochemical staining using DAKO EnVision system , histochemical stains including hematoxylin eosin and Gordon Sweet's reticulin methods were used.

采用免疫组织化学DAKOEnVison法、组织化学网质纤维染色和HE染色及形态学观察的方法，报道 1例极罕见于年轻人小脑的多形性黄色星形细胞瘤，探讨其临床病理特点。

Morphology of the EPCs was observed, and the growth curve of the EPCs was investigated. Surface antigens of the EPCs were analyzed by the flow-cytometer. The capability of intaking the acetylated low-density lipoprotein of the EPCs was detected using fluoresencent chemical method. The vasoformative capability and genetic stability of EPCs were cultured in matrigel, and examined by karyotype analysis.

观察培养细胞的形态，测定其生长动力学，采用流式细胞仪及免疫细胞化学方法检测其表型，应用细胞荧光化学法分析EPCs对乙酰化低密度脂蛋白(acetylated low-density lipoprotein， ac-LDL)的摄取功能，Matrigel上培养并检测其形成血管能力，染色体核型分析其遗传稳定性，裸鼠实验、软琼脂实验观察致瘤性。

Methods Healthy Sprague-Dawley rats received basal ovalbumin-sensitized combined with intra-esophageal mucosal acid exposure. The neuronal expressions of c-fos proto-oncogene were detected with immunohistochemical counter-staining and computerized color image analyzer.

采用腹腔注射鸡卵清蛋白基础致敏联合食管酸灌注的方法处理SD大鼠，并采用免疫组织化学方法和显微图像分析技术评价内脏高敏感性-食管化学刺激大鼠模型的可靠性。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。