免疫化学

Alkaline phosphatase was used as the label in this CLIA and a species of adamantane derivative was used as the substrate and the luminescence was detected by automatic luminometer.

采用碱性磷酸酶标记，金刚烷衍生物发光，自动化学发光分析仪测量等，进行乙肝病毒五种标志物化学发光免疫分析方法学研究。

Staining results of ADSCs: The extracellular matrix Alcian blue staining, Safranin O/Fast Green staining and collagen Ⅱ immunohistochemistry were positive.

脂肪间充质干细胞诱导后的细胞化学染色结果：诱导后脂肪间充质干细胞胞外基质阿尔新兰染色、蕃红O/固绿染色、和Ⅱ型胶原免疫细胞化学着色阳性。

To isolate mononuclear cells from foetus umbilic cord blood, After MNCs were incubated in the presence of APS group (APS 200 mg·L-1) and control group for 24 h,the cells were stimulated with Epo（5 IU/mL） for 0,2,5,30 min; STAT5 was measured by ICC and laser confocal scanning microscope, JAK2、STAT5 in nucleus and cytoplasm were measured by western-blotting-ECL in each group at different groups.

分离纯化胎儿脐带血单个核细胞，在常规体外培养体系加入APS（200 mg·L-1）作用细胞24 h，促红细胞生成素分别刺激0，2，5，30 min，免疫细胞化学与激光共聚焦显微镜观察细胞STAT5的表达；Western-blotting-增强化学发光法检测细胞JAK2与浆和核中STAT5的表达。

Methods: cell biological assay, histochemical staining of senescence associated-β-galactosidase and immunohistochemical staining of proliferating cell nuclear antigen wereused. Results: chondrocytes exhibited various types of amitotic figures.

应用细胞生物学方法、衰老相关-β-半乳糖苷酶组织化学染色和增殖细胞核抗原免疫组织化学方法，研究正常成人和正常成体大鼠气管软骨组织动力学。

At the same time seventeen normal ampullary oviducts during mid-secretory phase were included as control group. The routine two-step immunohistochemical technique was performed.

采用电化学发光法检测术前血β-HCG及免疫组织化学二步法测定各组标本中VEGF的表达，根据阳性率和表达强度进行组织化学评分和相关性分析。

Methods: A 5ml bone marrow was extracted from the lilac of human volunteers. By Percoll fluid and density gradient centrifugation, the MSC was obtained; after the cells filled the bottom of vessel, subcultured them, when they subculture in third generation, redigested them, 500 R/min centrifugate, alter the completed medium to chemical definition medium, examined the form change and prolifration of cells by invert microscope, toluidine blue stain、immunocytochemical stain and RT-PCR to test the type Ⅱ collagen mRNA and proteoglycan.

取健康成人髂后上棘处骨髓5ml，经percoll液分离后密度梯度离心，〓／ml密度接种培养，观察原代细胞的贴壁、增殖状况，细胞长满瓶底后进行传代培养；传至第三代细胞，重新消化后以500转／分钟轻度离心5分钟，〓／ml接种，改用化学限定培养基代替完全培养基培养，倒置显微镜观察细胞生长情况及形态变化，甲苯胺蓝染色观察诱导细胞合成细胞外基质中的蛋白多糖，免疫细胞化学染色检测ECM中Ⅱ胶原的蛋白合成，RT-PCR鉴定诱导细胞Ⅱ胶原mRNA的表达。

Thunberg Fritillary Bulb is one of the most popular Traditional Chinese herbs. Some trials in vivo and animals indicated that Peimine and Peiminine, extracted from Thunbery Fritillary Bulb, have the function of reversing MDR of two different kinds of mechanisms. They can improve the cytotoxic of ADR to cancer cells by 5～10 folds under concentration of 10% cancer cell growth inhibitory rate. Peimine and Peiminine not only feather typical character of modifier of MDR, but also differ from other moldifiers such as Calcium channel blocker, Cyclosporines in chemical structure, they belong to cevine groups alkaloid, a subgroup of C-Nor-D-homosteroid alkaloid.

浙贝母是临床常用中药，体外及动物试验证明，浙贝母中的有效活性成分贝母甲素和贝母乙素具有逆转肿瘤细胞MDR的作用，而且能逆转两种不同机制的多药耐药肿瘤细胞的耐药性，在无明显细胞毒剂量下，可使阿霉素对耐药肿瘤细胞的杀伤活性提高5-10倍，不但具备明确的多药耐药逆转剂的特征，而且在化学结构上属异甾类生物碱中的瑟文类生物碱，完全不同于钙离子拮抗剂、免疫抑制剂和其它现有的耐药逆转剂，具有作用靶点宽、化学结构独特的特点。

Part one Isolating, culturing, chondroblast induction and evaluation of human MSC in vitroObjective:To examine the property and proliferation ability of primary culturing MSC ,mvestgate the effect of lightly centrifugation and chemical definition medium to induce MSC differentiate into chondroblast.

结果：原代骨髓基质干细胞易于体外培养，增殖旺盛；经轻度离心和化学限定培养基诱导后细胞呈高密度生长，仍保持较旺盛的增殖能力，细胞转化成圆形肥大细胞，甲苯胺蓝染色显示软骨细胞外基质 v的特异性异染，11胶原蛋白免疫细胞化学染色呈强阳性，BT干CR鉴定显示＊胶原mRNA丰富的表达。

The aim are:lTo examine the proliferation ability and potential chondroblast differentiation ;2To find an ideal condition stimulated BMSC differentiate into chondroblast;3To examine the chondroblast proliferation in porous scaffolds and to explore the interaction between cells and materials;4To examine the release of cytokine in vitro.

取健康成人略后上棘处骨髓 5ml，经 percoll液分离后密度梯度离心，10'砌l密度接种培养，观察原代细胞的贴壁、增殖状况，细胞长满瓶底后进行传代培养；传至第三代细胞，重新消化后以500转／分钟轻度离心 5分钟，10V加 l接种，改用化学限定培养基代替完全培养基培养，倒置显微镜观察细胞生长情况及形态变化，甲苯胺蓝染色观察诱导细胞合成细胞外基质QCM）中的蛋白多糖，免疫细胞化学染色检测ECM中＊胶原的蛋白合成，RT干CR鉴定诱导细胞＊胶原mRNA的表达。

Then, the proliferative ability of OPCs in vitro was also investigated with the MTT assay.4. The contusive injury of spinal cord in a rat model was produced by the means of the weight-drop impact of Allen's method. The experimental animals were randomly divided into 4 groups, i.e. OPCs transplant group, transplant control group, plain injury group and sham operation group.

OPCs在体外化学条件培养基中继续生长，通过相差显微镜连续观察和免疫细胞化学技术检测研究分析体外条件下OPCs的定向分化规律和分化成熟情况，并采用MTT检测法研究分析OPCs在体外条件下的分裂增殖能力。4。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。