免疫化学

Methods The model of refractory epilepsy was established by kindling of amygdale. The expression of PGP in cerebra was observed by RT-PCR, immunohistochemistry and western blot.

方法建立杏仁核电刺激点燃的难治性癫癎大鼠模型，用RT-PCR、免疫组织化学及免疫蛋白印记的方法，分析比较PGP蛋白在癫癎模型组与正常对照组的表达。

Methods: The study was designed to observe SS and influence of serum CD_4~+、CD_8~+、IL-2、IL-6、TNF-α, splenic lymphocyte transformation rate, the expression of SS、SSTR-2 in spinal cord、PAG、NRM and hypothalamus the coexistence of SS and enkephalinergic neuron in back corner of spinal cord, the coexistence of SS and 5-HT neuron in NRM by using methods of behavioral nociceptive threshold test, measure of foot's cubage, flowcytometry, MTT, radioimmunity, HPLC-ECD, immunohistochemistry, laser confocal microscope on FAC-induced hyperalgesia.

以弗氏完全佐剂诱发的炎症痛模型大鼠为研究对象，应用行为学痛阈观察、足跖容积测定、流式细胞检测法、MTT法、放射免疫法、高效液相电化学法、免疫组织化学技术法、激光共聚焦技术等方法，观察SS和电针对痛阈、大鼠CD_4~+、CD_8~+、IL-2、IL-6、TNF-α水平和脾淋巴细胞转化率等指标的影响，以及SS、SSTR-2在脊髓和PAG、NRM及下丘脑部位的表达、在脊髓SS与脑啡肽能神经元的共存现象及在NRM中与5-HT能神经元的共存情况。

Methods LN in mouse endometrium was shown and measured with indirect immunofluorescence techniques, immunohistochemical ABC method and image analysis system. The effect of LN on the implantation was studied by using LN-antibody intra-uterine injection.

应用间接免疫荧光法、免疫组织化学ABC技术及图像分析法进行LN定位和定量测定；利用子宫内注射LN抗体的方法探讨LN对小鼠胚泡植入的作用。

Insulin cells (insulin immunoeractive cells, B-cells) in the splenic lobi of the pancreas were labelled from 0 to 365 days old shao ducks with ABC immunohistochemical method.

用ABC免疫组织化学方法，标记0～365日龄绍鸭胰腺脾叶内胰岛素免疫反应细胞，用显微测微尺测量B细胞胰岛的面积，作统计学分析。

Methods Immunohistochemistry and double-immunofluorescence techniques were used to detect the expressions of MMPs and TIMPs in 5 normal prostate and 34 prostate carcinoma tissues.

采用免疫组织化学、免疫荧光双重染色、形态定量分析方法对5例正常前列腺组织和34例前列腺癌组织MMPs及TIMPs进行定性、定位、定量检测。

The pathological changes and number of immunoreactive neurones were observed,and the activity of ChAT was detected by immunohistochemistry.

免疫组织化学方法检测大脑切片胆碱乙酰转移酶免疫反应活性及阳性神经元数量及形态学改变。

Rabbit digestive tract were investigated by the method of immunohistochemical technique and histochemical technique (toluidine blue or neutral red staining). There was immunoreactive positive reaction in rabbit digestive tract except in esophagus part.

利用免疫组化技术和组织化学技术，对家兔消化道5-HT免疫反应阳性细胞、肥大细胞进行了研究，并通过邻片法比较探讨了2种细胞的对应关系。

METHODS Dinitrofluorobenzene was used to induce the contact dermatitis in mouse ear, rhIL-11 0.375-1.5 mgkg^(-1)d^(-1) sc for 10 d, the degree of skin inflammatory reaction was observed. At the same time, the serum level of tumor necrosis factor-α and interleukin-6(IL-6) was measured by radioimmunoassay; the serum level of nitrogen monoxidum was detected by biochemical method and the expression of intercellular adhesion molecule-1(ICAM-1) was detected by immunohistochemical method.

采用2，4-二硝基氟苯致小鼠耳部皮肤接触性皮炎试验模型，观察sc rhIL-11 0.375~1.5mgkg^(-1)d^(-1)，连续10d后皮肤炎症反应程度，同时采用放射免疫分析法测定血清中肿瘤坏死因子-α和白细胞介素-6(IL-6)的水平，采用生物化学检测法测定血清一氧化氮的水平，采用免疫组化法测定皮肤中细胞间粘附分子(ICAM-1)的表达。

Objective: To compare HBsAb measurements of 1 472 children serum samples between chemiluminescent microparticle immunoassay and ELISA, and to analyze if the positve rate of HBsAb is related with gender or age.

目的：比较化学发光微粒子免疫分析法与酶联免疫法检测1 472例广州地区儿童HBsAb结果的差异，并探讨HBsAb的阳性率与儿童年龄、性别的相关性。

While applying OUS to the analysis of Yupingfen powder, I extracted and separated chemical components of each herb in the way of 3- routes approach with referrence to the variable results of the Indian ink clearance function of reticulo-endothelial system in mice. This experiment reflects YPFP's representative pharmacological effect of enhancing immunity.

在玉屏风散的实践中，依照三个分离路线对各药材的化学部位分别进行提取和分离，并以其功能主治中具代表性的&对机体的免疫调节作用&为重点，选择能够反映免疫功能增强作用的小鼠碳廓清药理实验结果为参照，历经三步实验，初步确定了玉屏风散的有效部位单元群。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。