免疫化学

ABSTRACT Objective To discuss the clinical pathologic features and differential diagnosis of mixed germ cell sex cord stromal tumorwith malignant variant of germ cell tumor Methods The clinical pathologic datum and immunophenotype were studied in the case of ovary MGCSCST with malignant variance of germ cell tumor Results The patient's genital anatomy and female phenotype were normal,karotypes was 46xx with uterogestation The tumor tissue consisted of mixed germ cells and sex cord stromal cells with evident malignancy of mixed germ cells There was no atypical structure of gonadoblastoma in tumor tissue Conclusion MGCSCSST has complex morphosis,thus,to prevent misdiagnosis,more samples should be collected and more slices be cut The patient may have prognosis malo when there are high malignant variants of mixed germ cells in tumor tissue

目的 探讨混合性生殖细胞性索间质肿瘤(mixed germ cell sex cord stromal tumor，MGCSCST)伴恶性生殖细胞肿瘤变异型的临床病理特点及鉴别诊断。方法卵巢MGCSCST伴恶性生殖细胞肿瘤变异型的病例进行临床病理特征和免疫表型分析。结果患者生殖器的解剖结构和女性表型正常，染色体组型是46xx，足月妊娠。肿瘤组织由混合性生殖细胞和性索间质肿瘤构成，混合性生殖细胞有显著的恶性特征。瘤组织中没有典型的性腺母细胞瘤结构。结论 MGCSCST有复杂的形态结构，因此更多的取材和切片可以防止误诊，当肿瘤组织中的混合性生殖细胞有高度恶性变异时，患者预后不良。卵巢肿瘤；生殖细胞性索间质肿瘤；免疫组织化学

The quantity and appearance of Foslike immunopositive neurons in ventrolateral part of PAG were investigated by immunocytochemical staining method.

采用免疫组织化学ABC法对大鼠中脑冰冻切片进行染色，显微镜下进行观察并拍照，对Fos免疫阳性细胞进行形态与计数分析。

Methods: immunohistochemical assay was used to detect cox 2 in 29 cervical cancer, 14 chronic cervitis and 18 normal cervixes. immunohistochemistry and in situ hybridization were used to detect the cox 2 protein and mrna in cervical cancer cell lines: siha and hela. the relationship between the expression of cox 2 and the clinicopathologic parameters of cervical cancer was analyzed.

用免疫组化方法检测29例宫颈癌患者cox 2的表达水平，并以14例慢性宫颈炎症患者和18例正常宫颈上皮为对照组；用免疫细胞化学方法和原位分子杂交法检测宫颈癌细胞株siha和hela中cox 2蛋白及基因表达。

Methods A model of autoimmune inner ear disease was established by systemically immunizing the guinea pig with the homologous crude inner ear antigen. The immunized cochleae and normal control cochleae were examined for the expression of hsp70 with techniques of immunohistochemistry and in situ hybridization.

利用同种异体内耳抗原免疫豚鼠，以建立自身免疫性内耳病（autoimmune inner ear disease， AIED动物模型，并应用免疫组织化学及原位杂位技术，研究热休克蛋白（heat shock protein， hsp70在正常对照组及 AIED 模型动物实验组耳蜗中的表达情况。

At the end of 2 months,6 months,9 months,12 months,respectively,left eyeballs of the mice for each group were enucleated to make tissue sections for light study and immunohistochemistry analysis.

取2 月龄、6 月龄、9 月龄、12 月龄C57BL/KsJ db/db糖尿病小鼠的眼球，制成石蜡切片，行免疫组织化学染色，进行免疫定位及用计算机图像分析仪对视网膜神经节细胞层的AngⅡ进行灰度和面密度分析。

The distribution of fibronectin in human oocyte and 2-cell to morulae embryos was also investigated, and the possible roles of fibronectin in fertilization, the development of early embryo and implantation were also discussed.

应用免疫组织化学方法和图像分析技术研究整合素β_3、FN和ER、PR在输卵管粘膜内的表达和变化；采用间接免疫荧光技术对FN在人卵母细胞和早期胚胎内的表达分布进行观察；光镜观察输卵管粘膜上皮细胞的形态学变化。结果：1。整合素β_3在人正常输卵管粘膜上皮纤毛细胞的胞浆内表达。

The quality concentrations of serum E2 were detected by radioimmunoassay. Pituitaries were embedded by paraffin and sectioned continuously. The morphologic figures of pituitary were shown by hematoxylin-eosin stain, the expression of estrogen receptor and progesterone receptor were detected by immunohistochemistry.

术后40 d麻醉后处死动物，放射免疫法检测血清雌二醇质量浓度，垂体组织常规石蜡包埋，连续切片，分别进行苏木精-伊红染色和雌、孕激素受体免疫组织化学染色。

The ulnas of fetal mice were cultured for 48 hours in BGJb medium treated respectively with estradiol and purearin. After fixing long bones and decalcification, paraffin sections were made, and then performed immunohistochemical staining for determining the level of typeⅠcollagen protein in growth plates. Positive cell locations in the resting zone, proliferative zone and hypertrophic zone of epiphyseal plate were observed under light microscope.

剥离出胎鼠尺骨，置于BGJb培养基中，葛根素和雌二醇作用48 h后，将长骨固定，脱钙后作石蜡切片，进行免疫组织化学染色，光镜下观察骺板静止区、增殖区和肥大区阳性细胞定位，图像分析系统下测定免疫反应阳性细胞面积。

Experiment 1, let rats acquire taste preference to quinine solution, and take account of β-EP-immunoreactive neurons in different nucleuses of hypothalamic areas, separately adopted taste preference learning training and immunohistochemistry; Experiment 2, Added banana essence into quinine solution to discuss the connection between flavor preference learning and the expression of β-EP; Experiment 3, To research endogenous opiate like substances contribute to the establishment of CTP and influence the behavior of taste preference by naloxone was i.p.

实验一，采用味觉嗜好学习训练和免疫组织化学方法，让大鼠获得对奎宁的味觉嗜好，计数了下丘脑不同核团内β-内啡肽免疫反应细胞数；实验二，向奎宁溶液中加入香蕉气味，探讨风味嗜好学习与下丘脑β-内啡肽表达的关系；实验三，腹腔内注射纳络酮，探讨内阿片肽对CTP建立及味觉嗜好行为表达的影响。

Fifiteen experiment animals were divided randomly into normal control group, 12h-post-trauma group and 36h-post-trauma group (5 each). Immunohistochemistry and image analysis techniques were used to measure the area, optic density value and integrated optic density value and integrated optic density value of Ngb immunoreactive products in primary somatosensory cortex (S1) and retrosplenial granular b cortex, which was located in the inner side and out side of traumatic brain injury cavity in coronal section of rat brain respectively.

采用自由落体硬膜外撞击方法建立脑外伤模型，15只实验动物分为正常对照组及脑损伤12、36h组，应用免疫组织化学和图像分析技术观察伤灶周围第一躯体感觉皮质区(S1)和压部后颗粒细胞b皮质区内Ngb免疫反应阳性信号面积、光密度和累积光密度的变化。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。