免疫化学

We used the avidin-biotin complex technique and EGFR monoclonal antibody to evaluate the expression of EGFR in 29 cases of cholesteatoma and 34 samples of normal postauricular skin. Of patients with cholesteatoma, 79%(23 cases) had EGFR-positive cells in the basal layer, 66%(19 cases) in the parabasal layer, and 62%(18 cases) in the upper layer of the epithelial tissue.

本研究利用免疫组织化学酵素抗体法（avidin-biotin-peroxidase complex，ABC method）以 EGFR 单株抗体免疫染色，评估29例胆脂瘤与34例耳廓后皮肤两者组织上皮内 EGFR 阳性细胞之分布表现。

Methods DNA was ext racted from paraffin-embedded tissue blocks of 44 angioimmunoblastic lymphaden o pathy patients and analyzed with polymerase chain reaction for IgH and TCRγ gene rearrangement. Immunohistochemistry staining was used to detect p53 protein expression.

方法运用聚合酶链反应技术检测44份AIL石蜡包埋组织标本中IgH及TCR γ基因重排情况，采用免疫组织化学ABC法染色技术检测AIL免疫表型及p53蛋白表达。

The choleratoxin B labelled cells distributed in the lateral hypothalamic area,the lateral preoptic area and the arcuate nucleus.

免疫组织化学染色显示，在大鼠下丘脑外侧区、外侧视前区和弓状核都可见CTb标记免疫阳性神经元。

The distribution of γ-aminobutyric acid and serotonin (5-HT) immunoreactive neurons in the protocerebrum and deutocerebrum of the butterfly Minathyma schrenckii were studied with the Colophony-paraffin embedding serial section technique and the Streptavidin-peroxidase immunohistochemical method.

采用树脂石蜡(Colophony-Paraffin，CP)组织包埋切片技术和链霉菌抗生物素蛋白-过氧化物酶(Streptavidin-Peroxidase，SP)免疫组织化学方法，首次研究了 GABA 和5-HT 免疫阳性神经元在白斑迷蛱蝶前脑和中脑中的分布。

METHODS: Pterygial specimens harvested from clinical operations received morphology, immunohistochemistry and immunofluorescence under a confocal microscope.

对手术切除的翼状胬肉标本进行形态学、免疫组织化学和免疫荧光共聚焦显微镜观察。

To observe the change of the SP- and CGRP- immunoreactive fibres in the pulpal tissue of rat by immunohistochemical method.

利用免疫组织化学方法观察大鼠牙齿开髓后2h、4h和急性牙髓炎2h、4h组牙髓组织中SP、CGRP免疫阳性纤维的变化。

Viral vector mainly contain adenovirus, retrovirus,adeno-associated virus and so on, but it has potential danger of safety; it isrepeled by immune system when it is injected to organism for a greatimmunogenicity. An injection with adenovirus vector with highconcentration, it leads to serious inflammatory reaction of liver. Viralvector such as liposome and polycation are commonly used lately. But,liposome and polycation have low specificness and targetness of genetransfer tissue, have lower transfection efficiency and short period ofgene expression, for they can be phagocytized by endothelial system.

许多的载体，病毒载体和非病毒载体以前已广泛应用，病毒载体主要包括腺病毒，逆转录病毒，腺相关病毒等；但病毒载体在安全性方面存在潜在的危险；免疫原性比较强，注射到机体后很快会被机体的免疫系统排斥，当静脉注射高浓度的腺病毒载体会使肝脏发生严重的炎症反应；非病毒载体目前常用的有脂质体及多聚阳离子聚合物；但脂质体和阳离子聚合物介导基因转移缺乏组织的特异性和靶向性，转染效率较低且易被网状内皮系统吞噬，基因表达时间短；因此研制新型的非病毒载体已成为研究的热点，纳米颗粒具有小尺寸效应，表面效应，随着颗粒直径变小，比表面积将会显著增大，故具有很高的化学活性，因而纳米成为了最有应用前景的非病毒载体。

The gray value of SP and CGRP radioactive particles in bilateral spinal trigeminal caudal subnucleus in rats were detected by immunohisto- chemisrty while obvious damages in trigeminal neurocytes on the injected side were confirmed.

在确认实验侧三叉神经节细胞出现明显破坏的同时，采用免疫组织化学技术，检测大鼠双侧三叉神经脊束核尾侧亚核内P物质和降钙素基因相关肽免疫阳性颗粒的灰度值。

The prolif- erative ability of CD133 + tumor cells in vitro was estimated by MTT assay, and the proliferative ability of CD133 +, CD133 - and control SUNE cells were statistically compared. Finally, the immunocytochemical staining method and flow cytometry were used to detect differen- tial ability of CD133 + tumor cells in vitro .

采用免疫荧光细胞化学技术及流式细胞仪检测鼻咽癌细胞株SUNE中CD133的表达情况以及CD133+细胞体外分化能力；免疫磁珠分选技术纯化CD133+肿瘤细胞；MTT法检测CD133+细胞的体外增殖能力，并将其与CD133-及未分选细胞进行比较。

Methods Sixty adult healthy male Wistar rats were enrolled in the experiment. PTSD-like rat model was established by single prolonged stress. There was no SPS stimulation in the control group and the rats of the other five groups were undisturbedly raised for 24 hours, 4 days,7 days,14 days and 28 days respectively after SPS stimulation.The expressions of glucocorticoid receptors in the locus ceruleus nucleus of all groups were detected with Nissl staining, immunohistochemistry,Western blotting and PCR, and image analysis and statistical analysis were performed.

成年健康雄性Wistar大鼠60只，使用无连续单一应激方法建立PTSD大鼠模型，分别取SPS处理后24h、4d、7d、14d和28d大鼠蓝斑组织，同时取正常蓝斑组织作为对照，应用尼氏染色观察蓝斑形态，采用免疫组织化学、免疫印迹和RT-PCR方法分别观察及检测各组蓝斑神经元GR表达的变化，并进行图像分析和统计学处理。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。