免疫化学

Methods SECs were isolated by collagenase perfusion combined with isopycnic centrifugation in a two-step percoll gradient and selective adherence, and identified by morphologic observation under light microscope, by immunocytochemistry for CD14 and Ⅷfactor related antigen, by immunofluorescence staining for RECA-1 and fenestrations under SEM.

方法用胶原酶灌注结合Percoll密度梯度离心加选择性贴壁的方法分离SEC；用光镜形态学、Ⅷ因子和CDl4免疫细胞化学染色及RECA.1单抗间接免疫荧光法及SEM鉴定SEC；采用TEM及AnnexinV联合PI双染的方法检测SEC的凋亡。

Bone marrow mesenchymal stem cells could differentiate into osteoblasts, adipocytes and neural like cells with osteoblast inductor (β-sodium glycerophosphate, dexamethasone, vitamin C), lipoblast inductor (dexamethasone, 3-isobutyl-1-methylxanthine, bovine insulin, indometacin) and serum-free medium inductor (dimethyl sulphoxide, butylated hydroxyanisole) respectively. Osteoblast marker (alkaline phosphatase, osteocalcin mRNA, calcium node), adipocyte marker (lipid droplet, PPAR γ-2mRNA) and neural cell-like marker (nissl body, neuron specific enolase, neurofilament protein) were respectively determined by the immunohistochemical method, polymerase chain reaction and immunocytochemical method.

分别采用成骨细胞诱导剂（β-甘油磷酸钠，地塞米松，维生素C）、成脂肪细胞诱导液（地塞米松，甲基异丁酸黄嘌呤，牛胰岛素，吲哚美辛）及二甲基亚砜和羟基丁酸苯甲醚无血清培养基诱导剂干预细胞向成骨、脂肪、神经细胞分化，经免疫组织化学染色、PCR、免疫细胞染色方法检测成骨标志物（碱性磷酸酶、骨钙素mRNA、钙结节）、脂肪标志物（脂滴、PPARγ-2mRNA）、以及类神经标志物（尼克氏体、神经烯醇化酶、神经丝蛋白）。

The effects of antibiotic barrier (physical and mucilaginous elimination) and joint prevention in the antibacterial immunity system of Eisenia fetida were analyzed in this study by applying animal immunology, ecology and biochemistry. The main results of this study were as the followings.

本研究以动物免疫学、生态学、生物化学为指导思想，以蚯蚓的生态免疫系统为主线，探讨了赤子爱胜蚓的体壁屏障系统(包括皮肤的物理排除作用和随体表粘液分泌排除)和协同防御作用在其免疫系统中的作用。

This experiment take one-day-old chickens as the object of study, we apply cell raise technologyand MTT method, the indirect enzyme-lined immunosorbent assay, the immunity enzymehistochemistry methods andα-naphthyl acetate esterase etc..

本实验以1日龄雏鸡为研究对象，应用细胞培养技术和MTT法、间接酶联免疫吸附实验、免疫酶组织化学和组织酸性α-醋酸萘酯酶染色法等方法。

Were comparativey studied using immunocytochemical tech niques-peroxidase antiperoxidasemethod.These positive immunoreactive systems were compared with the neuroanatomical data on neuropile organization and single nerve cell type in the bee's brain.

通过免疫细胞化学方法－PAP法，研究了中华峰和意大利峰脑和视叶中乙酰胆碱、5－羟色胺（5－HT）、γ－氨基丁酸免疫反应阳性神经元胞体和纤维的形态、染色强度、分布和各种神经丛之间的联系。

Methods The neuroepithelial stem cells were isolated and cultured by microdissection, mechanical blowing and serum-free suspension culture.

方法采用显微解剖、机械吹打、无血清悬浮培养方法分离培养神经上皮干细胞，采用巢蛋白免疫细胞化学染色技术检测神经上皮干细胞，用NSE和GFAP免疫组化染色检测并计数神经细胞和神经胶质细胞。

Methods 20 cases of brain tissues of intractable epilepsy and 15 cases of nonepileptic brain tissues were collected.

按随机化原则，在我们建立的难治性癫痫患者术后脑组织库中抽取15例患者的颞叶脑组织，用免疫组织化学、免疫荧光法分别检测其NCAM-140kDa的表达，并与对照组进行比较。

Methods 45 patients with nonobstructive azoospermia, 40 patients with obstructive azoospcrmia and 40 healthy subjects were involved in this study. Serum FSH, LH and T levels were measured by chemiluminescence immunoassay, and serum INHB was measured by sandwich enzyme-linked immunosorbent assay.

采用化学发光免疫分析法测定45例非梗阻性无精子症、40例梗阻性无精子症及40名正常生育男性血清FSH、LH及T的浓度，以双抗体夹心酶联免疫吸附试验测定血清INHB水平。

Results There was significant negative correlation between the Nogo-A expression and the morphological change of tumor tissues according to immunohistochemistry. Western-blot analysis also indicated that the gray value of Nogo-A protein band in the oligodendroglioma group was significantly higher than that in the anaplastic oligodendroglioma group.

结果 免疫组织化学结果显示，Nogo-A免疫阳性染色的光密度值与肿瘤细胞核分裂、核异型比例呈明显的负相关，对蛋白印迹试验结果进行光密度分析也发现少突胶质细胞瘤中的Nogo-A蛋白条带灰度值明显高于间变型少突胶质细胞瘤中的灰度值。

The Research of the equine diseases and the Analysis of their reasons in Henan Province;2. Epidemiological investigation about equine tumors in Gansu province;3. The results showed that prepubertal orchidectomy of the equine resulted in a slight but not significant increase in the number and percentage of gonadotrophs,but significantly increased the average area of .

利用免疫组织化学和形态计测学的方法，观察了 7匹公马和 6匹在 1～ 2岁期间被摘除了睾丸的阉割马的脑垂体前叶促性腺激素细胞的数量和面积，同时利用放射免疫分析方法检测了血浆中垂体促卵泡激素和垂体促黄体激素的水平。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。