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免疫化学

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METHODS: A total of 129 gastric mucosa biopsies and its corresponding serum specimens were collected from patients with superficial gastritis (n=30), gastric ulcer or erosion (n=35), atrophic gastritis (29), and gastric cancer (n=35). The expression of PGC in the gastric mucosa was detected by immunohistochemistry, and the concentration of serum pepsinogen A and pepsinogen C were determined by enzyme linked immunosorbent assay.

129例胃黏膜活检组织及其血清标本(浅表性胃炎30例,胃黏膜糜烂溃疡35例,萎缩性胃炎29例,胃癌35例),采用免疫组织化学染色检测胃黏膜标本中PGC的表达情况;采用酶联免疫吸附实验检测血清PGA和PGC含量。

To search for the significance of immunohistochemical markers for the differential diagnosis of rhabdomyosarcoma and the clinical impact of different subtypes of rhabdomyosarcoma on prognosis.

目的 :探讨横纹肌肉瘤的诊断和鉴别诊断中免疫组织化学标记的意义及不同类型横纹肌肉瘤对预后的影响。方法:应用病理形态学和免疫组化方法对 47例横纹肌肉瘤进行研究。

The distribution of SYP and the ultrastructures of SYP immunoreactive cells and immunoreactive products in intercellular space of the sacculus rotundus of rabbits were investigated using immunohistochemistry and immune electron cytochemistry methods.

采用免疫组化和免疫电镜细胞化学的方法,对家兔圆小囊组织中的SYP阳性细胞和胞间阳性反应物的光镜和电镜超微结构特点进行了研究。

The ultrastructures of the SP, VIP secretory cells showed there were several kinds of cells with different morphology which could express these neuropeptides in sacculus rotundus.

免疫电镜细胞化学观察发现在兔圆小囊内除了DNES等细胞内有SP和VIP的分布以外,在细胞间隙和淋巴细胞、巨噬细胞等的胞浆膜上也常见SP和VIP免疫电镜阳性反应信号。

3Chondrogenesis was assessed using Alcian blue staining, Toluidine bluestaining, Safranin O/Fast Green staining at 7 and 14 days, still collagenⅡimmunohistochemistry and aggrecan immunofluorescence at 4, 7 and14 days after initial chondrogenic induction of adipose -derivedmesenchymal stem cells.

3观察脂肪间充质干细胞在诱导后的生长增殖情况、形态变化,于诱导7、14天后应用阿尔新兰染色、甲苯胺兰染色、藩红0/固绿染色,以及4、7、14天后应用Ⅱ型胶原蛋白免疫细胞化学、aggrecan免疫荧光评价其成软骨能力。

The extracellular matrix wasstained positively for Alcian blue, Toluidine blue and Safranin O / FastGreen at 7 and 14 days after initial chondrogenic induction of adipose -derived mesenchymal stem cells, and indicated collagenⅡand aggrecanwere expressed positively.

2脂肪间充质干细胞在高密度的"微团"培养条件下,于诱导7、14天后阿尔新兰染色、甲苯胺兰染色、藩红0/固绿染色阳性,诱导4、7、14天后Ⅱ型胶原蛋白免疫细胞化学、aggrecan免疫荧光阳性表达。

Methods The different expression of CK in SHEE and SHEEmt cell lines,was observed by the immunocytochemistry and Western blotting.

采用免疫细胞化学染色和免疫印迹的方法,观察SHEE细胞和SHEEmt细胞中CK的表达。

METHODS Using immunohistochemistry method, we observed the Fos expression in the brain of purified protein derivative of tuberculin immunized mice at different time points.

用免疫组织化学方法观察纯化的结核菌素衍生蛋白免疫刺激后不同时间点小鼠脑内的 Fos蛋白表达。

To explore the clinical reliability and usability of the acridinium ester-labeled CLIP and ELISA in detection of AFP to establish a specific, effective, simple and reliable detecting method in our lab.

[目的]探讨吖啶酯标记的化学发光免疫测定(chemiluminescence immunoassay, CLIA)分析技术与酶联免疫吸附试验一步法两种方法检测甲胎蛋白(Alpha-fetoprotein, AFP)的临床可靠性、适用性。建立本室特异、有效、方便、可靠的试验方法。

Pituitaries were stained by HE and immunocytochemical methods. Adrenocorticotropic hormone in rat blood plasma was measured quantitatively with radioimmunoassay.

对垂体进行HE和免疫细胞化学染色;应用放射免疫分析法检测血浆促肾上腺皮质激素含量。

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