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That to explore the general biologic mechanism of acupuncture through observation of ERK1/2 and P38 is a new try of studying the mechanism of acupuncture in the fascial point of view.

综上所述,本研究分别采用了HE染色、免疫组化、免疫印迹等技术手段,从针刺作用于机体的始动环节——针体对浅筋膜的牵拉和对肌肉的刺激着手,区别于以往的&穴位刺激——靶器官反应&的对于针灸研究的传统模式,且从观察ERK1/2和P38两种信号蛋白变化的角度,来探讨针刺作用于机体在生物学层面的普遍的机制,这是从筋膜角度研究针刺机理的一个新的尝试。

Healthy dogs were inoculated with rabies vaccine and antibody titre was measured by ELISA and FAVN method from the 14th day. The immunized dogs were challenged with rabies virus BD06 strain.

将40只犬免疫兽用狂犬病疫苗后,第14 d静脉采血分离血清,分别采用ELISA法和国际贸易指定试验FAVN法检测血清样品,试验犬免疫第21 d攻击狂犬病毒BD06株。

Results: The study showed that the earliest stromal nodules were fibrous, and with the dual differentiation of myofibroblasts toward both smooth muscle cells and fibrous cells, they developed into fibromyomatous nodules and muscular nodules. The results of reclassifying the 55 nodules immunohistochemically were that 23(42%) was fibrous, 28(51%) fibromuscular nodules and 4(7%) muscular.

结果:结合HE切片和免疫组化结果发现前列腺间质结节的早期为幼稚的纤维性结节,随着结节内肌纤维母细胞向平滑肌细胞和纤维细胞的双向分化,间质结节可继续演化为纤维肌性结节和肌性结节。55个结节按免疫组化分类,纤维性结节占42%,纤维肌性结节占51%,肌性结节占7%。

AIM: To determine whether immunization with recombinant filarial chitinase or a fragment containing the epitope recognized by McAbMF1 and SXP 1 could protect jirds against microfilaremia resulting from infection with B.malayi.

目的:测定重组马来丝虫壳质酶(r-chitinase)及其片段和SXP-1抗原免疫接种沙鼠后是否具有抗马来丝虫微丝蚴血症的保护性免疫作用。

PartⅡ: Analysis of the paraffin embeded tissue slides [obtained from trail and control group at pre-treatment (d0) and post-operation (d15±1 days) ] and frozen section slides (from 4 typicalcases: 1 of IAC group, the other sBRM group at d5, d7 and d10), by H-E stain, monoclonal antibodies (CD3/CD4/CD8/CD68/CD57) marking, SP-DAP or APAAP-AEC stain, and TUNEL in situ apoptotic cell DNA fragments fluorescin labeling.

二。组织病理学研究:对上述两组病人治疗前后原发灶肿瘤组织蜡块分别作H-E和CD3、CD68表型免疫组化染色;并对其中四例典型病人的疗中间断连续活检新鲜组织作H-E染色,CD3、CD4、CD8和CD57表型免疫组化染色和凋亡细胞DNA断片TUNEL法荧光标记检测。

Methods Gastric cancer tissues, adjacent tissues and normal gastric mucosal tissues in 75 patients were studied, The expression of ubiquitin and 26S protesome activity were analyzed by Wes tern blot and fluorogenic technique, res pectively.

对75例胃癌患者分别在胃癌、癌旁及远端正常黏膜组织取样进行研究。采用免疫印迹方法分析泛素表达,免疫荧光方法分析265蛋白酶体活性。

Methods The study was performed concering the neurons and fibers of SP,CGRP and VIP-immunoreactive substance in three predominant superfical ganglial plexus of the guinea pig heart by immunohistochemical ABC method and observed by light microscope.

应用免疫组织化学ABC法,对豚鼠心脏表面3个主要神经节丛的神经元及神经纤维进行了SP、CGRP及VIP免疫组织化学染色及光镜观察。

Using immunofluorescence double label method to observe GAP-43 expression of jugular ganglionic SP positive cell after the onset of asthma.

四、应用免疫荧光双标技术,在哮喘模型上观察了哮喘发作后,颈静脉节SP免疫阳性细胞GAP-43的表达变化。

David Elliott, a gastroenterologist and immunologist at the University of Iowa, indicate that intestinal worms, which have been all but eliminated in developed countries, are "likely to be the biggest player" in regulating the immune system to respond appropriately, Dr.

艾略奥特博士的研究表明,已在发达国家全部消除的肠道寄生虫,对调节免疫系统作出适当反应&可能是起最大作用的&,艾略奥特博士在一次采访时说道,细菌感染和病毒感染似乎以同样的方式影响着免疫系统,但力度不同。

Gibel carp were inoculated with formalin killed Aeromonas hydrophilaand fed with diets supplemented with appropriate amount of yeast β-glucan. 28 days after the inoculation, the infulence of dietary yeast β-glucan on the resistance of gibel carp against Aeromonas hydrophila were determined by examing their gained weight, agglutinating antibody titer, lysozyme activity, phagocytic activity and relative percent survival by challenging with live A.

在饲料中添加定量的酵母β-葡聚糖,投喂经注射接种福尔马林灭活的嗜水气单胞菌(Aeromonas hy-drophila)菌苗的异育银鲫28 d后,通过测定供试鱼的增重量、血清中凝集抗体效价、溶菌酶活性、谷丙转氨酶活性、血清总蛋白含量、白细胞吞噬活性以及活菌攻毒后的免疫保护率,探讨了酵母β-葡聚糖对受免异育银鲫免疫应答的增强作用。

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In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是,在否定句或疑问句里,这种带有"do"的方法表达的效果却没有什么强调的意思。

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