免疫

A double antigens Sandwich Enzymelinked Immunosor-bent Assay( DAgS -ELISA)have been developed under the basis o f higher titer and freeze dried production conjugate of Brucella antigen with horseradish peroxidase. Serumsamples formdiagnosed patients of Brucellosis and sheep,cattl e,swine infected with Bru-cella as well as normal men and those a nimals in laboratory detected throu gh DAgS -ELISA,SAT and RBPT tests.

方法在制备高滴度布氏菌抗原辣根过氧化物酶结合物及其冻干制品基础上，应用酶联免疫吸附双抗原夹心法即双抗原夹心酶联免疫吸附试验、试管凝集试验及虎红平板凝集试验对从山西省布氏菌病疫区收集的布氏菌病患者、布氏菌感染羊、牛、猪以及健康人、羊、牛、猪共计290份血清进行对比检测。

The former stimulates the cells and transfer the signals into the cells through the ligation with receptors on the cell membrane. The latter, a derivative of nucleotide, causes a cytotoxic effect to suppress thymidylate synthetase activity, and, therefore, to damnify RNA and DNA synthesis.

前者通过与细胞表面的受体结合，把胞外刺激传递到胞内，诱导免疫受体介导的信号级联放大作用，引起一系列免疫效应；后者是是小分子的核苷酸衍生物，可直接进入细胞内，通过抑制胸苷酸合成酶及损伤RNA、DNA等对基因转录和核苷酸代谢产生影响，最后产生细胞毒效应。

Methods:120 cases of intestinal tract biopsy tissues were carried on tissue fixation,deaquation,transparence,steeping wax,hematoxylin staining and immunohistochemistry using lightwave-microwave combination Ⅱ in lightwave-microwave oven.

选择肠道活检组织120例，组织固定、脱水、透明、浸蜡、苏木精染色和免疫组化染色用光波-微波组合Ⅱ在光波-微波炉中进行，同时以全自动组织脱水机处理组织和常规免疫组化染色作为对照。

Objective Establishment of a hybridoma cell line secreting a monoclonal antibody to facilitate iˉdentification of human tissue kallikrein（KLK1gene,hK1protein）.Methods Mice were immunized with E.coli-exˉpressed GST-hK1fusion protein and their spleen cells were fused with SP2/0myeloblastoma cells.Specificity of the monoclonal antibody was shown by Western blotting and by immunofluorescence.Results The monoclonal antibody reacted specifically to E.coli-expressed hK1and with the KLK1cDNA-transfected COS-1cells.

目的 将人组织激肽释放酶（基因命名 KLK1，酶命名为hK1）cDNA克隆入肠杆菌表达质粒，以重组菌表达的GST-hK1融合蛋白免疫小鼠，获得了分泌hK1特异单克隆抗体的杂交瘤细胞株方法将KLK1cDNA克隆入真核表达质粒，用获得的重组质粒转染COS-1细胞，经间接免疫荧光试验证明，上述单克隆抗体能与转染细胞发生特异反应。

We examined the properties of DC associated with immune suppression in NOD mice by insulin injection subcutaneously and ability to suppress diabetes transfer by diabetogenic effect cells in secondary NOD severe combined immunodeficiency recipients.

NOD小鼠皮下注射牛胰岛素诱导免疫抑制，以血糖检测、胰腺组织学分析和胰岛beta细胞凋亡分析观察自身抗原的免疫保护作用，分离骨髓树突状细胞细胞，流式细胞术分析细胞表面抗原，混合淋巴淋巴细胞反应测定细胞功能。

TFC could remarkably inhibit the ear edema induced by dimethylbenzene in mice at the dose of 125,250,500mg.kg~(-1), the swelling of rats hind paws induced by carrageenan and primary inflammatory reaction in AA rats at the dose of 84,168,336 mg.kg~(-1) and reduce the weight of granuloma induced by cotton in rats at the dose of 84,168,336mg.kg~(-1), respectively.

TFC的免疫调节作用体外研究发现，TFC0.005，0.05，0.5，5，50 mg·L~(-1可明显提高环磷酰胺(cyclophosphamid，Cy)诱导免疫功能低下小鼠的脾淋巴细胞增殖反应，而对正常小鼠的脾淋巴细胞增殖无明显作用。

The results showed that orexin-like immunoreactiveneurons were observed in the medial preoptic nucleus,periventricular nucleus,paraventricular nucleus,supraoptic nucleus,dorsomedial nucleus,perifornical nucleus,mammillary nucleus,anterior area,lateral area and posterior area of the hypothalamus,with majority in the lateral area,supraoptic nucleus and mammillary nucleus and with minority in t...

结果表明，在猪下丘脑内，开胃素A免疫阳性神经元分布于下丘脑的视前内侧区、室周核、室旁核、视上核、背内侧核、穹隆周核、乳头体核、前区、外侧区和后区等部位，以下丘脑外侧区、乳头体核和视上核出现的免疫阳性神经元最多，以室周核的最少。这一结果与在其他动物上获得的基本相似。

The current study represents the first example of cytokine expression in fish induced by an ectoparasitic infection and indicates the importance of localised mucosal immune reactions in responses of fish towards gyrodactylids.

本文首次对体外寄生虫感染诱发的鱼类细胞因子表达进行了研究，显示了鱼体针对三代虫产生的免疫应答中，局部粘膜免疫的重要性。

The appearance of facial eczematous lesions in babies gradually changed tolichenoid lesions on the limbs and popliteal fossae of children.

患儿嗜酸性粒细胞、IgE、总花环、活性花环及表面膜免疫球蛋白细胞的变化说明患儿存在免疫功能的紊乱。

The effect of oral infection and subsequent challenge with Eimeria vaccines on the populations of CD4 , CD8 T lymphocytes in the intestine, cecal tonsils and spleen of chickens was assessed by immunohistochemistry.

用免疫组化ABC法检测鸡用上述球虫进行第一、二次免疫后小肠或盲肠、盲肠扁桃体和脾脏中CD4~、CD8~ T淋巴细胞的动态变化。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷