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Three immobilization modes of antigen to the polymers in the pH-sensitive phase separation immunoassay were investigated and compared. The results showed that the immobilization mode in the presence of N-ethyl-N'-(3-dimethylaminopropyl)carbodiimide hydrochloride rendered the most desirable results. The immobilization efficiencies and immunological reaction activities of immobilized antigen of this mode were improved over the other two modes.

本文研究并比较了pH敏感相分离免疫分析中抗原在高分子上的三种固定化模式,结果显示由碳二亚胺偶联的固定模式效果最好,固定化效率、固定抗原的免疫反应活性较之另外两种模式均得到了提高。

Ion-exchange chromatography with DE-52 was used for the purification of the antigens. The purified products were characterized by Western-blotting. Result For the pollen extract of Caryota ochlandra, 30 protein bands were detected in SDS-PAGE.

收集过敏病人血清,采用免疫印迹(Western-blotting)法鉴定其变应原成分,通过离子交换层析对花粉变应原进行初步纯化和免疫印迹鉴定。

Methods By using to turn differently to carry on centrifugence to the same batch of specimen with time soon,and then sing ELISA examination HBsAg.

检测乙肝表面抗原,常采用酶联免疫吸附试验夹心法以及胶体金免疫层析法。

Another argument is that apes chimpanzee immunodeficiency virus contact time is not long, the virus carried by chimpanzees may be red top zanthopygia monkey, Cercopithecus monkeys or carried by other types of immunodeficiency virus variant from the new.

另一种说法是,黑猩猩接触猿类免疫缺陷病毒的时间不长,黑猩猩所携带的病毒可能由红顶白眉猴、长尾猴或其他种类猴子所携带的免疫缺陷病毒新近变异而来。

The preparation of immunopeptides hydrolyzed from Cervus elaphus antler blood by papain was studied.

选用木瓜蛋白酶对天山马鹿茸血进行水解制备免疫活性肽,确定了最佳水解工艺条件,并测定了三种不同水解度水解物的抗氧化活性和免疫活性。

OBJECTIVE: This study aimed to investigate the immunological rejections of APA microencapsulated chromaffin cells transplanted into rat anterior chamber of eyes and tendon of feet, and to evaluate the immunoisolated effect of microencapsulation.

目的:观察海藻酸钠-多聚赖氨酸-海藻酸钠微囊化嗜铬细胞移植到大鼠眼前房和足胝部的免疫排斥反应,评价微囊化技术的免疫隔离作用。

38 patients with nasal polyp and 15 control cases were chosen. Their IL-5 concentration in tissue homogenate were measured by enzyme-linked immunosorbent assay. All samples were stained with Chromotrope 2R histochemical and SP immunohistochemical method separately to examine eosinophil and IgE+ cells in nasal polyp.

方法采用免疫组化SP法检测38例鼻息肉,15例正常鼻腔粘膜组织中IgE阳性细胞表达情况;采用酶联免疫吸附试验检测以上病例中IL-5含量;采用Chromotrope 2R特染方法检测以上病例中Eos浸润情况。

The immune function of the patients with filarial chyluria was impaired in terms of the changes in T-lymphocyte subsets.

丝虫性乳糜尿患者机体免疫系统的比例失衡,免疫调节机能出现异常,且呈抑制趋势。

But there are few or no visible F-actin ring of blocking proteinase being released in MC of tryptase and chymase immunoreactivity MC(subscript TC and MC of chymase immunoreactivity MC(subscript C. Cell appearance was inflated and intracellular proteinase had been released.

而类胰蛋白酶和类糜蛋白酶免疫反应性的肥大细胞及类糜蛋白酶免疫反应性的肥大细胞内少见或未见明显的阻碍蛋白酶释放的丝状肌动蛋白环;细胞形态膨胀,细胞内蛋白酶已释放。

The chymase levels were ditermined by using the method of enzyme-linked immunosorbant assay in 45 cases of chronic hepatitis. Mast cell distribution in the specimens was determined immunohistochemically by using anti-chymase antibody.

采用酶联免疫吸附实验检测45例慢性肝炎肝组织中Chymase浓度,观察不同的纤维化分期(S1-S4),其肝组织中Chymase浓度;用免疫组织化学染色法观察Chymase单克隆抗体标记的肥大细胞分布情况。

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In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是,在否定句或疑问句里,这种带有"do"的方法表达的效果却没有什么强调的意思。

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