免疫

On the other hand, mice primed with all the microsphere formulations showed higher anamnestic responses than those primed with soluble tetanus toxoid. Higher anamnestic response means stronger protection from infection.

另一方面，用微球免疫过的小鼠再次接受低剂量抗原时，所产生的免疫记忆抗体高于用破伤风类毒素溶液免疫过的小鼠，说明微球具有更强的免疫保护力。

We use the kidney to research the issue of that cystamine can regulate kidney to improve the disease or not. In flow cytometry, we use CD8/CD4/CD25 makers to observe the balance of TH1/TH2, and we find that cystamine can raise TH2 expression to produce autoantibodies and also can induce overexpression of Treg to ease inflammation.

为了同时在免疫方面观察胱胺是否也有著能减缓SLE的倾向，进而深入了解观察肾脏这个重要的次级免疫器官，探讨cystamine是否对於肾脏也具有重要的调节作用进而能改善病症，在本实验中以flow cytometry对免疫倾向的确认，透过CD8/CD4/CD25 maker观察TH1/TH2 balance，发现胱胺能有效的反转SLE大量倾向TH2进而产生的自体抗体，同时也能增加 Treg的高量表达进而去抑制免疫反应缓解发炎。

Compared with control,①the mean tumor weight of H22 of SXKA Granules three dose groups were decreased significantly(P.01,P.05),and the mean inhibition rates of SXKA Granules 20、10 g/kg dose groups were above 30%;②the mean tumor weight of S180 of SXKA Granules three dose groups were decreased significantly(P.01),and the mean inhibition rates of SXKA Granules 20、10 g/kg dose groups were above 32%;③the mean tumor weight of EAC of SXKA Granules three dose groups were decreased significantly ( P.01, P.05),and the mean inhibition rates of SXKA Granules 20 g/kg dose groups were above 38%;④the mean tumor weight of Lewis carcinoma of SXKA Granules three dose groups were decreased significantly(P.01, P.05),and the mean inhibition rates of SXKA Granules 20、10、5 g/kg dose groups were above 36%;⑤the mean tumor weight of W256 of SXKA Granules three dose groups were decreased significantly ( P.01, P.05),and the mean inhibition rates of SXKA Granules 20、10 g/kg dose groups were above 32%;⑵Compared with control,SXKA Granules 20、10 g/kg dose groups had extended the survial time of the P388-bearing mice respectively(P.01),and the mean prolong rates of SXKA Granules 20、10 g/kg dose groups were above 50%;⑶Compared with S180-bearing group, SXKA Granules 20、10 g/kg dose groups could increase the weight of thymus and spleen, Spleen index and thymus index were increased, SXKA Granules 5 g/kg dose group could increase thymus index(P.05);⑷As Compared with control group, SXKA Granules 20、10 g/kg dose groups could improve mouse serum half hemolysis value depressed by transplanted tumor dramatically(P.01), which revealed the SXKA granules could improve the mouse humoral immunity system;⑸SXKA Granules 20 g/kg dose group could increase of englobe indexαon S180-bearing mice remarkably(P.01), which indicated the SXKA Granules could improve their cellular immunity system.

对荷W256大鼠，生兴克癌冲剂20、10、5 g / kg三组的平均瘤重明显低于对照组(P.01，P.05)，生兴克癌冲剂20、10 g / kg组的平均肿瘤抑制率均大于32 %；⑵与空白对照组相比，生兴克癌冲剂20、10 g/ kg能显著地延长移植小鼠白血病P388小鼠的存活天数(P.01)，生兴克癌冲剂20、10 g/ kg对荷白血病P388小鼠生命延长率均在50%以上；⑶与S180荷瘤组相比，生兴克癌冲剂对荷瘤鼠的免疫器官重量、胸腺指数和脾指数有一定的提高趋势，其中生兴克癌冲剂5 g / kg组对荷瘤小鼠的胸腺指数有一定的提高作用(P.05)；⑷与S180荷瘤组相比，生兴克癌冲剂20、10 g /kg组可提高由荷瘤引起的小鼠血清半数溶血素值的降低(P.01)，表明其可提高荷瘤小鼠体液免疫功能；⑸与对照组相比，生兴克癌冲剂20 g /kg组可提高荷S180肉瘤小鼠的免疫吞噬系数α值(P.01)，表明其可提高荷瘤小鼠细胞免疫功能。

According to the above evaluation of immuno-epidemiology of RSA, RSA may be etiologically classified into lack of materno-fetal immuno-recognition, materno-fetal inappropriate immuno-recognition, and disorder of materno-fetal immuno-recognition.

根据RSA免疫流行病学分析，RSA的病因可以分类为：母-胎免疫识别低下型、母-胎免疫识别过度型及母-胎免疫识别紊乱型。

Results The SP-IR and CGRP-IR neurons could not be found in the above-mentioned ganglial plexus.But SP-IR fibers distributed sparsely,CGRP-IR fibers were more densely than SP-IR fibers,especially A-PGP.The population of VIP-IR neurons and fibers were most dense and interweaved ganglial plexus.

结果 在上述三个神经节丛内，未发现SP及CGRP免疫反应阳性神经元，但可见少量SP免疫反应阳性神经纤维分布，CGRP免疫反应阳性神经纤维数量较SP阳性纤维密集，尤其在A-PGP更为明显；VIP免疫反应阳性神经元和神经纤维分布最为密集，交织成神经节丛。

As a kind of insect, Heliothis armigera has its special immunity system,which is made up of by cellular immunity and humoral immunity. Phenoloxidase plays an important role in insects humoral immunity.

棉铃虫和其它昆虫一样，具有自己独特的免疫系统，由细胞免疫和体液免疫组成，其中酚氧化酶在体液免疫中占有重要地位。

vcr (0.2 g/ml) and fe3o4 nano-magnetic ferrofluid have no harmful effects to hematogenic immunologic function.

vcr（0.2 g/ml）和纳米级 fe3o4 磁流体对血细胞的免疫功能都没有不良影响，甚至磁流体还可以提高血液免疫反应中白细胞的免疫功能，为红细胞包载磁流体和抗癌药物治疗肿瘤提供实验依据，为抗癌药红细胞载体靶向抗肿瘤免疫提供新的治疗途径。

Taken together, the heterogonous boost combination provided superior protection by stronger CD(superscript 4+)(Th1) and CD(superscript 8+) T cell mediated immune response, which suggested that it will be a promising regimen for MTB vaccine development.

结果表明，DNA初发免疫、BCG加强免疫法能显著提高小鼠CD(上标 4+)，CD(上标 8+)T细胞介导的免疫应答，增强小鼠抗结核杆菌感染能力，是提高结核病疫苗免疫效果的新途径。

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

It is demonstrated CAV 2 adenovirus and named CAV 2 XN 3,after analysing the morphological,physical,chemical and biological features.The authors replicate the virus into hypotoxic and immunize 8 local young canines of two months old,without harmful reactivity being found.

实验将该病毒复制成弱毒疫苗，免疫 8只 2月龄当地幼犬，经临床观察证明，试验犬未出现不良反应；免疫后 14天采血测定血清中和抗体效价达到 1∶12 8，免疫后 2 5天血清中和抗体效价达到 1∶2 5 6 ，免疫后 10个月降至 1∶32 ，但仍能耐受强毒攻击，而对照组 4只犬则发病死亡。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。