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Methods : To study the therapeutic effect of Shubuhuo on 30 case of Hyperplasia of M- ammary Gland after three months,including the improving of clinical symptom and the size of adenoncus of the mammary gland.The Sex hormones of lood was evaluated by radioimmunoassay,including serum estrogen(E2)、progestogen、prolactin,T-cell tubgroup(CD3、CD4) was evaluated by flow cytometry,to find the diffence between pretherapy and post-treatment in luteal.

运用疏补活汤治疗30例乳腺增生病患者3个月(1个疗程)后,观察其临床疗效,包括治疗前后患者临床症状的改善以及乳腺近红外线扫描确定乳房肿块大小的变化;在治疗前后于患者黄体期用放射免疫测定法检测血清性激素水平的变化,包括雌二醇(estrogen,E2)、孕激素(progestogen,P)、泌乳素(prolactin,PRL),同时用流式细胞仪检测治疗前后细胞免疫功能(T细胞亚群CD3、CD4)的变化。

Strongly positive immunostained thyroid follicular cells of EAT rats for TRAIL mainly distributed adjacently to infiltrating lymphocytes. Staining of infiltrating lymphocytes for TRAIL was found to be rather weak or even absent.

桥本甲状腺炎大鼠中TRAIL免疫染色强阳性甲状腺滤泡细胞多分布于浸润淋巴滤泡附近,浸润淋巴细胞中TRAIL免疫染色较弱。

Albicans infection.Methods We observedarately adoptively immunized with T lymphocyte or antibody or phagocyte.

分别观察过继免疫T 淋巴细胞、抗体和吞噬细胞的免疫功能低下小鼠抵抗腹腔白念珠菌感染情况。

In heat shock protein gp96-peptide complexes induced tumor rejection model,antigen specific CD8T cells adoptively transffered into perforin knock out mice failed to proliferate and differentiate into CTLs whereas co-transfer of wide type NK cells rescued the clonal expansion and CTL differentiation of transferred CDST cells.These results suggested important immuno-modulatory roles of perforin and presumably different roles of perforin secreted by NK cells versus by CD8~+ CTLs.

例如,在热休克蛋白Gp96-肽复合体所诱导的抗肿瘤免疫应答中,穿孔素基因敲除小鼠中单独回输的野生型CD8T细胞不能产生有效的抗肿瘤免疫应答,而同时回输野生型NK细胞能够恢复CTL应答。

Pituitaries were stained by HE and immunocytochemical methods. Adrenocorticotropic hormone in rat blood plasma was measured quantitatively with radioimmunoassay.

对垂体进行HE和免疫细胞化学染色;应用放射免疫分析法检测血浆促肾上腺皮质激素含量。

The same result can also be achieved when optimizing F8 function (Rastrigin's function). Comparing to EGA, KEGA is easy to realize and the increase of computation load can be neglected, but the performance of KEGA can be improved greatly. 2. To enhance the speed of AIA, two new methods of computing the concentration of antibody were developed. Simulation results for F8 and F15 (needle in haystack: type I) functions show that the two methods can improve the running speed of AIA more than 8. 5 times. 3. As to the drawback in defining antibody concentration, a new definition based on Euclidean distance between two antibodies and fitness is proposed. On this basis, a new AIA was constructed. The convergence performance are more better than that of AIA. 4. Combining king crossover with AAIA and DBAIA, two new AIAs, KAAIA and DKBAIA, were proposed.

仿真实验结果表明KEGA实现简单,与EGA相比,其增加的计算开销可以忽略,但性能改善十分明显; 2、针对基于信息熵的免疫算法运行速度慢的缺点,提出了二种能够加快AIA运行速度的新的抗体浓度计算方法,得到两种新的免疫算法,即加速的人工免疫算法(Accelerated Artificial Immune Algorithm,AAIA)和加速的人工免疫算法-2(Accelerated Artificial Immune Algorithm-2,AAIA-2),对测试函数F15(needle in haystack:type I)、F8函数的仿真研究表明,这两种算法的运行速度都是AIA的8.5倍以上; 3、针对AIA的浓度定义存在的缺陷,提出了一种新的基于抗体间欧氏距离和适应度的新的抗体浓度定义,并依据该定义构造了一种基于欧氏距离的人工免疫算法(Euclidean Distance-based Artificial Immune Algorithm,DBAIA)。

It was used for detection of virus RNA in AIV infected chicken embryo allantoic fluids clinical specimens from various avian.

建立并优化了检测禽流感病毒核酸的探针杂交法,探针杂交法能鉴别出非免疫鸡胚和SPF鸡胚尿囊液中的病毒,攻毒后第 3天的 SPF和非免疫鸡泄殖腔拭子中 AIV的最大检出率为 1/10 ,对临床样品中的 AIV的最大检出率为 1/7,而直接 HA和 HI法及 AGP试验检不出临床样品的 AIV。

Methods: the first experiment Immunetolerance in mice sciatic nerve allograft by intrathymic injection of allogene: 48 mice were divided into 4 groups randomly: Group A,Group B, Group C and Group D(nerve allograft under immunosurppressant).

研究技术路线:第一部分:小鼠胸腺内注射异基因抗原在同种异体坐骨神经移植免疫耐受中的作用 1、动物分组:48只Balb/c小鼠随机分为4组,A组;B组;C组;D组(异体神经移植加用免疫抑制剂组)。

At the beginning the number was decrease rapidly, then slowly. There were still some MSCs alive 8 week after transplant. Conclusion: The mouse's MSCs can survive more than 8 week in subcutaneous tissue of allogene mouse. MSCs were hypoimmunogenic.

小鼠MSCs植入同种异体小鼠皮下8周后仍能存活,MSCs在体内保持低免疫原性的特点,同种异体与同种同基因MSCs在诱导机体免疫应答方面无明显差异。

ConclusionThe first experiment Immunetolerance in mice sciatic nerve allograft by intrathymic injection of allogene : the specific immune tolerance can be induced in the sciatic nerve allograft by intrathymus injection of MHC allogene.

研究结论:第一部分:小鼠胸腺内注射异基因抗原在同种异体坐骨神经移植免疫耐受中的作用胸腺内注射异基因MHC抗原可诱导异体坐骨神经移植的特异性免疫耐受。

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