免疫

A wide range of topics are covered,including fundamental research on animal immune systems; new techniques and developments in veterinary immunodiagnosis; immunogenetics; immunology and immunopathology of parasitic and infectious disease; and clinical immunology in animals.

该期刊覆盖的主题包括：动物免疫系统的基本研究；兽医免疫诊断的新技术和发展；免疫遗传学；寄生生物引起的疾病和传染病的免疫学和免疫病理学等。

Abstract］ objective to compare the advantages and disadvantages of different samples and different methods in detection for banded krait venom.methods double immunodiffusion and enzymelinked immunosorbent assay were used as the methods to detect different samples of people and animal who were bit by banded krait.results in double immunodiffusion the samples were all negative;with elisa the samples of wound tissue liquid and blood were all positive.conclusion wound tissue liquid and blood are both fitting samples in detection for banded krait venom;double immunodiffusion isn't adapt to detect banded krait biting patient,but elisa is an good detection method.

目的 探讨银环蛇毒中毒检测中不同材料与不同方法的优缺点。方法采用双向免疫扩散法与间接elisa法对银环蛇毒中毒的人与动物的不同标本进行检测。结果双向免疫扩散法死者伤口皮肤组织液、死者心脏血清、死小猪伤口皮肤组织液及死小猪血清标本均未见免疫沉淀带；elisa法测标本均为阳性。结论银环蛇毒中毒检测中伤口皮肤组织液和血液都是合适的材料；双向免疫扩散法的检出率较低，而elisa法则是一种较好的检测方法。

These data suggest that vaccination with DNA encoding amastin confers protectiveimmunity to mice infected with Leishmania donovani and the mice injected withpcDNA3.1-ctxB/amastin got stronger protective immunity.

结果表明：含amastin基因的两种DNA疫苗均可在小鼠体内产生一定的免疫保护效应，ctxB/amastin融合基因免疫组的免疫保护效果似乎强于单纯的amastin基因免疫组。

The luminescence intensity of suspended immune cells (1×10〓 cells/ml) from peripheral blood, anterior kidney and spleen of grass carp, which were from two groups immunized with being injected Aeromonas hydrophilia and unimmunized respectively, were detected by means of BPCL4 Intelligentized Ultraweak Luminometer, the datum that were dealt with statistics demonstrated that luminescence intensity of the cells from all kinds of immune organs and blood were intensified after the fish were immunized in two weeks and the luminescence intensity from anterior kidney cells was the highest, mitogens had the enhancing effects. Luminol could strengthened luminescence intensity.

把未免疫的和经过嗜水产气单胞菌注射免疫的草鱼外周血、前肾、胸腺和脾脏以及它们的悬浮细胞（密度控制在1×10〓个细胞／ml左右）分别用BPCL4智能化微弱发光测量仪测量各组的发光强度，结果显示：草鱼各类免疫器官与细胞在免疫两周后发光强度增加，肾细胞的发光强度最大；有丝分裂原具有增强作用；化合物鲁米诺能提高发光强度。

It includes four parts: 1 Part I, it introduces the basic knowledge of computer viruses such as computer virus definition, history and important viruses events, features, behavior phenomena, difference with computer software and hardware troubles, damage behavior and capability, classification, naming, future trend, etc; 2 Part II, it introduces the corresponding knowledge of computer systems related to computer viruses such as the composing of software and hardware of the computer system, storage media and its working mechanism, interrupt technologies,.com/.exe/.pe file formats and their working mechanism, etc; 3 Part III, it introduces computer virus mechanism and theory such as computer virus structure, work flow, working mechanism of key modules, typical technologies used to design computer viruses including the corresponding traditional technologies (interrupt filching, memory resident, etc.), the corresponding new routine technologies (self-encrypting, Mutation Engine, etc.), and the corresponding new technologies used by some prevalence computer viruses such as macro viruses, e-mail viruses, worm, hacker, Trojan, mobile phone viruses, the working mechanism analysis of some kinds of typical and prevalence computer viruses such as file-type viruses, macro viruses, e-mail viruses, worm viruses, hacker, Trojan, mobile phone viruses, the analysis of some typical and prevalence computer viruses examples including BALL viruses, WORD macro viruses, WantJob viruses, Code Red viruses, BO Trojan, etc; 4 Part IV, it introduces the defense and killing technologies of computer viruses such as the aim and criterion of computer viruses defense and killing, prevention methods and corresponding technologies, detection technologies (comparison method, character code scanning method, behavior inspecting method, analysis method, etc.), manual and automatic killing technologies, immunity technologies such as IBM digital immunity system, new anti-viruses technology trends (real-time anti-viruses technologies, 32 kernel technologies, active kernel technologies, etc.), some typical virus defense and killing softwares (Symantec AntiVirus product, PC-Cillin AntiVirus product, etc.), the defense and killing method analysis of some kinds of typical and prevalence computer viruses (file-type viruses, macro viruses, worm viruses, hacker, etc.), for example, firewall and intrusion detection technologies for anti-hacker, the defense and killing of some typical and prevalence computer viruses examples including WORD macro viruses, Code Red viruses, BO Trojan, etc.

课程内容具体包括四大部分：1）第一部分，介绍计算机病毒基本知识，包括：计算机病毒定义、病毒发展史及重大事件、病毒特点、病毒表现现象及与软硬件故障的区别、病毒破坏行为及危害性、病毒的传播途径及媒介、病毒分类、病毒的命名及计算机病毒技术发展趋势等内容；2）第二部分，介绍与计算机病毒有关的计算机系统相关知识，包括：计算机系统软硬件组成、存储介质结构及工作原理、计算机系统引导机理及流程、中断技术、。com/。exe/。pe等文件格式及工作机理等内容；3）第三部分，讲解计算机病毒机理，包括：计算机病毒的组成结构、病毒工作流程、病毒引导/触发/感染/破坏等模块的工作机理、计算机病毒所采取的编制技术（包括中断窃取/内存驻留等传统编制技术、自加密/隐形/变形机等新的常规编制技术、宏病毒/电子邮件病毒/网络蠕虫/特洛伊木马/黑客/手机病毒等一些新的流行病毒所采取的编制技术等）、一些类型的典型或流行计算机病毒的工作机理分析（包括：引导型病毒、文件型病毒、宏病毒、电子邮件病毒、蠕虫病毒、黑客、特洛伊木马、手机病毒等）、一些典型或流行的计算机病毒实例剖析（包括：小球病毒、WORD宏病毒、求职信病毒、红色代码病毒、冰河木马等）等内容；4）第四部分，讲解计算机病毒防治技术，包括：计算机病毒防治目的、病毒防治策略及规范、病毒在管理和技术上的预防措施、病毒检查技术（包括：比较法/病毒特征码扫描法/行为监测法/虚拟执行法/分析法等）、手工和自动病毒清杀技术、病毒免疫技术（包括：针对某种一次性感染病毒的基于病毒标签的免疫方法/基于自我完整性检查的计算机病毒免疫方法/IBM的数字免疫系统等）、反病毒技术的新发展（包括：实时反病毒技术/32位内核技术/主动内核技术/以毒攻毒技术等）、诺顿/趋势/金山等公司的病毒防治软件产品、一些类型的典型或流行的计算机病毒（包括：引导型病毒、文件型病毒、宏病毒、蠕虫病毒、电子邮件病毒、手机病毒、黑客、特洛伊木马）的防治措施（其中，也包括介绍面向防范黑客攻击的防火墙、入侵检测技术）、一些典型或流行的计算机病毒防治实例剖析（包括：WORD宏病毒、红色代码病毒、冰河木马v1.1/v2.2等）、多层次病毒防护体系等内容。

Part Ⅱ Objective: To study the immunoprotective and the immunotherapic effects of the new nucleic acid vaccine of the hepatitis B: pVAX1-C3d-S2S; respectively in normal and HBV transgenic mice after being inoculated intra- muscularly with this Eukaryotic Expression Plasmid, such as the in situ production of the expressed protein in muscle tissue section and specific humoral immune response in normal mice、the variation of specific antigen、antibody and HBV DNA, especialy the changes of specific antigen in liver.

第二部分新型乙肝核酸疫苗pVAX1 C3d-S〓S免疫预防效应和治疗作用的研究目的：研究含有新型分子佐剂补体C3d的HBV adw血清型的乙肝核酸疫苗重组真核表达质粒pVAX1-C3d-S〓S接种正常BALB／c小鼠和HBV转基因小鼠后，其免疫预防作用（接种局部肌肉中特异性抗原蛋白的表达和所诱生的体液免疫应答规律）以及免疫治疗作用（血清中特异性抗原的表达、特异性抗体的产生、HBV DNA的消长和肝组织中抗原表达的变化）。

The result indicated that the percentage of lymphocyte were decreased, the percentage of neutrophile granulocytes were magnified significantly with the lighting time increasing, which was due to the level of the melatonin inhibited in the long time lighting; and to supply different exogenous dose, induce the percentage of lymphocyte were magnifying, the percentage of neutrophile granulocytes were decreased. And the statistical analysis showed there was significant difference.

结果表明：在给予不同光照时间的条件下，随着光照时间的延长，褪黑素的分泌水平受到抑制，导致具有特异免疫功能的淋巴细胞百分率下降，具有非特异免疫功能的中性粒细胞百分率上升，经统计分析表明不同组间的测定结果均存在显着差异；采用24h全天光照，用以模拟机体切除松果腺的情况，再给予一定剂量外源性褪黑素，导致机体内具有特异免疫功能的淋巴细胞百分率上升，具有非特异免疫功能的中性粒细胞百分率下降，经统计分析表明不同组间的测定结果均存在显著差异，并且高剂量褪黑素对机体的影响更为明显。

RESULTS A eukaryotic expression system for high expression humanmutantCD59 were successfully set up : The recombinant PALTER-MAX plasmid containing human mutantCD59 cDNA and PCDNA plasmid were co-transfected into CHO cell by cation lipoid mediating method ;and the cells were grown in F12 medium containing 400ug/ml G418 for 14 days, positive clones were grown in RPMI1640 medium to get stable expressing cell lines . Highly expressing clones were selected by flow cytometry ,and were named PALTER-CD59-CHO1PALTER-CD59-CHO2 . Flow cytometry indicated that expression rates of PALTER-CD59-CHO1 and PALTER-CD59-CHO2 were 53.7%and 54.5%. Further more, Stable highly expressing CHO cell lines were more detected by immunocytochemistry and immunofluorescence technology . PALTER-CD59 -CHO1 and PALTER-CD59-CHO2 were grown in RPMI1640 to get a large of cells . CD59 protein were obtained by spalling PALTER- CD59- CHO1 and PALTER - CD59 - CHO2 cells . Stable highly expressing cells were further validated by SDS-PAGE, immunoblot analysis and solid enzyme immunoassay . PALTER - CD59 - CHO1 and PALTER - CD59 - CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER-CD59-CHO1 or PALTER-CD59-CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59 -CHOI or PALTER-CD59-CHO2 was higher than unglycated ones . PALTER -CD59-CHO1 and PALTER -CD59 -CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER - CD59 - CHOI or PALTER - CD59 - CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59-CHO1 or PALTER - CD5 9-CHO2 was higher than unglycated ones .

结果 成功构建突变人CD59的真核细胞表达系统：运用阳离子脂质体介导法将含有突变人CD59的PALTER—MAX重组质粒与PCDNA共转染入CHO细胞：用含有400ug／mlG418的F12培养基培养14天，筛选出稳定阳性表达克隆，RPMI1640培养基扩增获得稳定表达细胞株，并用流式细胞术进一步筛选出高效表达细胞株分别命名为PALTER—CD59—CH01、PALTER—CD59—CH02，表达率分别为53.7％、54.5％；应用免疫组化方法、免疫荧光技术进一步鉴定阳性细胞株；RPMI1640培养基大量扩增PALTER—CD59—CH01、PALTER—CD59—CH02细胞株，裂解细胞得到CD59蛋白质；通过SDS—PAGE凝胶电泳技术、免疫印迹技术、固相酶联免疫吸附试验验证了这两中文摘要个阳性细胞株CO59蛋白的高效表达；50mM核糖培养72小时，获得突变人CD59糖化细胞株，BCECF染料释放试验结果显示，PALTER一CD59一CHOI、pALTER一CD59一CHOZ细胞较PALTER一CHO细胞染料释放率低，未糖化PALTER一CD59一CHOI、PALTER一CD59一CHOZ细胞比较糖化后细胞染料释放率低。

A lot of experimental studies have demonstrated that inner ear is not an immunological privilege organ, as it possesses anatomic constitution of an immune defence system, and its perilymph contains immunoglobulin. In fact, inner ear is one of important immune response sites in hos 〓.

许多基础研究证实，内耳并非免疫豁免器官，它具有免疫防御系统的解剖学组成，外淋巴中存在免疫球蛋白，因而被认为是宿主重要的免疫应答场所之一[1]。

The results of immuhistochemistry show: compared with normal retina, more 3-NT-positive cells and iNOS-positive cells appear in inner nucleus layer of diabetic retina; less eNOS-positive cells appear in inner nucleus layer and vascular endoderm of diabetic retina; less nNOS -positive cells appear in inner nucleus layer of diabetic retina; more ET-positive cells, ETRA- positive cells, ETRB- positive cells appear in inner nucleus layer of diabetic retina; moreα- synuclein- positive cells appear in ocular cone and rod layer of diabetic retina. Conclusions 1. RFDD-PCR is an efficient technique for research diseases genomics as a mass screening to complete gene expression with the identifying of candidate gene related to disease.

免疫组织化学结果显示：13-NT和NOS：与正常视网膜相比，8周糖尿病大鼠视网膜中，INL的3-NT和iNOS免疫阳性细胞明显增多，INL和血管内皮层的eNOS阳性细胞明显减少，INL的nNOS阳性细胞也明显减少；2ET及ETR：与正常视网膜相比，8周糖尿病大鼠视网膜中，ET、ETRA、ETRB免疫阳性细胞明显增多，增多的阳性细胞主要集中于INL，而在血管内皮层增多不明显；3α-synuclein：与正常视网膜相比，8周糖尿病大鼠视网膜中，α-synuclein免疫阳性细胞明显增多，增多的阳性细胞主要集中于视网膜视锥视杆层。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。