免疫

Using the theories of probability, algebra and spectral theory comprehensively, we investigate some related characteristics of logic functions in cryptography: Firstly, we introduce m order generalized s - correlation immunity of Boolean vector functions and prove that the higher order generalized ε- correlation immunity can guarantee the lower order generalized ε- correlation immunity. Then by applying decomposition formula of joint distribution of Boolean random vectors, we give a spectrum criterion of m order generalized e - correlation immunity of Boolean vector functions. Furthermore, we show that the algebraic degree of m order generalized e - correlation immune Boolean vector functions is not restricted by the correlation immune orders.

本文主要运用概率论的思想和方法，并结合代数学和频谱理论的相关知识，对密码学中逻辑函数的有关性质进行了研究，主要包括以下三个方面的内容：首先，对布尔向量函数的相关免疫性进行了拓展，给出了k维布尔向量函数m阶广义ε-相关免疫的概念，证明了布尔向量函数的高阶广义ε-相关免疫性蕴含低阶广义ε-相关免疫性，并根据布尔随机向量联合分布分解式得到了布尔向量函数m阶广义ε-相关免疫的一个谱判别条件，还说明了m阶广义ε-相关免疫布尔向量函数的代数次数不受相关免疫阶数的制约。

VCR (0.2 g/mL) and Fe3O4 nano-magnetic ferrofluid have no harmful effects to hematogenic immunologic function. Magnatic ferrofluid can even elevate the function of leucocytes. The results can supply experimental foundation for erythrocytes to capsulate both magnetic ferrofluid and anticancer drug, and also can provide treatment pathway for anticancer drug erythrocyte carrier targeting antitum or immunity.

VCR（0.2 g/mL）和纳米级 Fe3O4 磁流体对血细胞的免疫功能都没有不良影响，甚至磁流体还可以提高血液免疫反应中白细胞的免疫功能，为红细胞包载磁流体和抗癌药物治疗肿瘤提供实验依据，为抗癌药红细胞载体靶向抗肿瘤免疫提供新的治疗途径。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length P1, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length P1, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3. 1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus , which were expressed in BHK-21 cells, were confirmed by sandwich-ELISA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3. 1/IFN which includes the gene IFN-α of cattle. Subsequently, Recombinant plasmids were injected to cattles with or without pcDNA3. 1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies titers were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫苗，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接/酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1/P12X3C和pcDNA3.1/P12X3C3D、pTARGET/P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1/IFN分别/同时免疫，第二次免疫后第三周豚鼠攻以1OOID〓或1000ID〓的O型FMDV China99株；随后将质粒pcDNA3.1/P12X3C、pcDNA3.1/P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1/IFN同时免疫牛，三周后经牛舌皮攻以10〓ID〓的O型FMDV China99株。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length PI, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length PI, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3.1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus, which were expressed in BHK-21 cells, were confirmed by sandwich-ELlSA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P 12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3.1/lFN which includes the gene IFN-a of cattle. Subsequently, Recombinant plasmids were injected to catties with or without pcDNA3.1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies liters were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫曲，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接／酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1／P12X3C和pcDNA3.1／P12X3C3D、pTARGET／P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1／IFN分别／同时免疫，第二次免疫后第三周豚鼠攻以100ID_(50)或1000ID_(50)的O型FMDV China99株：随后将质粒pcDNA3.1／P12X3C、pcDNA3.1／P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1／IFN同时免疫牛，三周后经牛舌皮攻以10~4ID_(50)的O型FMDV China99株。

The fusion DNA fragments of ag85b-mpb64 and ag85b-mpb64-esat-6 were obtained by PCR andSOE technique. Various DNA vaccines were constructed with the pcDNA3.1: fusion of two genes, and of three genes, bivalent combinations and trivalent combinations(pCA+pCM+pCE6). BALB/c mice were vaccinated with this DNA vaccines.The mice injected withBCG were positive control and the mice injected with pCDNA3.1 and PBS were negative control.The mice were immunized 3 times with 2-wk intervals. The animals in group BCG were only inoculatedsubcutaneously with 1×10~6 CFU BCG at initial vaccination. The serum IgG titers and IgG isotype weredetermined using iELISA coated with M. bovis PPD and rMAE protein expressed and depurated inprokaryotic expression system every week.

同样，利用PCR和SOE技术，获得牛分枝杆菌mpb64-ag85b和mpb64-ag85b-esat-6融合基因，以pCDNA3.1为载体构建了牛分枝杆菌多价组合和多基因融合DNA疫苗：二基因融合(pCDNA3.1-MPB64-Ag85B，简称pCMA)和三基因融合(pCDNA3.1-MPB64-Ag85B-ESAT-6，简称pCMAE)DNA疫苗；二价组合和三价组合(pCA+pCM+pCE6)DNA疫苗，免疫BALB／c小鼠，以牛分枝杆菌BCG免疫组为阳性对照，以pCDNA3.1及PBS免疫组为阴性对照，共免疫3次，每次间隔2周，BCG组仅初免时皮下免疫1次。1免后每周，以原核表达纯化的重组MPB64-Ag85B-ESAT-6蛋白和牛分枝杆菌PPD为包被抗原，以间接ELISA方法检测血清IgG水平及lgG亚类。

Result Obvious immunological rejection occurred after implantation of aortic and pulmonary homografts. Cellular and humoral immunological responses were involved in the process of chronic immunological rejection.

结果 低温保存同种带瓣管道移植后有明显的免疫排异反应发生，细胞和体液免疫参与免疫排异反应的过程；慢性免疫排异反应以细胞免疫为主。

Specific antibody of the turbot fries could be detected in a week after injection immunization and maintained high level for at least 6 weeks, with the highest of 1382,the relative percentage survival was 50%;The level of the specific antibody of the vaccinated brood turbots was low but maintained stable and kept long time.

其中大菱鲜鱼苗免疫鱼抗体效价在免疫后6周内一直呈上升趋势，最高达1382，4周后测得的免疫保护率为50%；大菱虾亲鱼免疫后抗体效价产生较慢，效价水平较低，但下降缓慢，免疫3个月后仍维持抗体效价54。

The titer test showed that the polyclonal antibodies immunonized by two immunogens all had high titer and the longer interval made for obtaining higher titer. The speciality test showed that the polyclonal antibodies immunonized by conjugations MQCA-RSA had the better speciality than the other one. Usage of RSA as the carrier protein could avoid producing antibody for carrier protein.

以两种偶联物为不同免疫原、采用不同的免疫时间间隔免疫新西兰大白兔，获得多克隆抗体，抗体效价测定和特异性鉴定的实验结果表明：两种免疫原均能产生高效价的抗体，免疫间隔延长有利于效价提高，且以RSA为载体有利于避免针对蛋白载体的抗体产生。

Western blots were performed to investigate the cross-reactivity of various labeled antibodies with serum proteins from other fish species. Results Immunoglobulins of five fish species were purified, including bighead carp, carp, argus snakehead fish, ricefield eel, sea bass. The titers of five rabbit anti-sera obtained up to 1:32 examined by agarose double immunodiffusion. Five kinds of purified rabbit anti-fish immunoglobulins antibodies were labeled with HRP and their activities were up to 1:10000 examined by EUSA. Four kinds of rabbit anti-fish immunoglobulins antibodies labeled with HRP could react with serum proteins from other fish species.

结果 纯化了鳙、鲤、乌鳢、黄鳝、鲈五种鱼血清免疫球蛋白，免疫双扩散法测定兔抗这五种鱼免疫球蛋白的抗血清效价均达到1：32，并对纯化的兔抗鳙、鲤、乌鳢、黄鳝、鲈五种鱼类免疫球蛋白的抗体进行了HRP标记，ELISA测定标记抗体的效价达到1：10000左右，Western blots显示标记抗体与部分其他鱼类免疫球蛋白之间存在不同程度的交叉反应。

A "Booster" shot refers to giving a vaccine more than one time. The follow-up vaccinations will BOOST the immune level up higher and the patient will be even better protected from the disease.

&加强免疫&是指给狗狗进行一次以上的免疫，再次的免疫可以提高狗狗的免疫水平，从而使之得到更好的免疫能力。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。