免疫

In conclusion, the non-specific immunity of Portunus trituberculatus was significantly affected by salinity low 14 and high 36 stress during 12 hours; PSVF2 has more immunity advantages in these non-specific immune factors than that of CPF1 under salinity stress.

总结：低盐和高盐胁迫对三疣梭子蟹的非特异性免疫影响显著且表现出时间效应；在正常和抗盐度胁迫下，筛选蟹F2比普通蟹F1在非特异性免疫因子方面更具免疫优势。

Objective To study the statement of immunoreaction of c Jun and functional recovery of spinal cord injury in rats after fetal spinal cord transplantation.Methods Experimental rats were divided into two groups:spinal cord hemisection injury combination with transplants of FSC to injured site group,and spinal cord injury that received gelfoam pledgets group.On the lst,3th,7th,14th and 28th day after operation,6 animals in each group at each time phase,were observed for their function recovery with the methods of praxiology and electro physiological examination,the animals were then sacrificed for the observation of the statement of c Jun reaction by immnochemistry method and computer image analysis system was used for quantitative analysis.

目的：研究大鼠胚胎脊髓移植后能否影响，c-Hun免疫反应的表达和脊髓损伤后大鼠功能的恢复，方法；将动物分为脊髓半切洞损伤加胚胎脊髓移植组和单纯脊髓板切洞损伤加明胶海绵填塞组，每个时相点每组6只动物，术只1，3，7，14和28天，应用行为学和电生理检查观察大鼠功能恢复情况，应用免疫细胞化学方法，c-Jun免疫反应的表达，采用计算机图像分析技术，进行定量分析。

GnRH-ir fibers distribute in the various brain region from the olfactory bulb to the cerebellum. They are especially abundant in the hypothalamus, the preoptic area and the ventral telecephalon. The immunopositive reaction in precocious fish is evidently stronger than that in unprecocious fish, and there is only very weak reaction in the gonadal undeveloped fish. The differences are most obvious in the hypothalamus, but not in other areas.

性早熟大黄鱼的神经内分泌细胞对GnRH抗血清的免疫阳性反应明显，无论在细胞的数量和免疫强度都比未早熟鱼多和强，在性腺尚未发育的幼鱼脑中仅有弱的免疫阳性，这种差异在下丘脑外侧结节核及视前核处最为明显，在其它GnRH阳性部位则差异不显著。

Passive transfer of sera from mice immunized with expressed G1 and G2 conferred partial protection or prolongation of time to death from fatal hantaan virus infection in infant mice which challenged with hantaan virus at 10LD50. The spleen cells from mice immunized with G1, G2and NP can protect or prolongate the lifetime of infant mice infected with lethal dose (10LD50 or 40LD50) hantaan virus.

将重组G1、G2和NP免疫小鼠的血清和脾细胞注入汉滩病毒致死性感染的乳小鼠体内，发现G1、G2的免疫血清和G1、G2及NP免疫脾细胞对乳鼠有保护作用，同时在一组试验中观察到G1的脾细胞可显著缩短乳鼠的存活时间。

In contrast with mucous cells, the lymphocytes in spleen and pronephric kidney performed positive for the mRNA of IgM VH detection.

上述观察表明，鲤鱼的皮肤粘液中存在的大量的免疫球蛋白是由粘液细胞分泌的，但是，粘液细胞自身不具备合成免疫球蛋白的能力，其细胞中的IgM要依赖于体内的免疫细胞。

Orange-spotted grouper is a benthic protogynous marine fish with sex inversion. It is one of the most important culturing fish in the southeast of Asia. As in other vertebrates, its growth is mainly controlled by growth hormone . Few data are available concerning the regulation of GH in grouper. In this paper, the regulation of growth hormone release from pituitary of orange-spotted grouper and its mRNA level was determined by radioimmunoassay and RNase protection assay.

应用分子克隆技术，构建了斜带石斑鱼生长激素(Growth hormone，GH)基因重组质粒，在大肠杆菌细胞中获得高效表达，亲和层析纯化斜带石斑鱼GH融合蛋白做为抗原，免疫家兔制备特异性抗体，通过酶联免疫实验、放射免疫测定、离体脑垂体静态孵育和液相杂交／核糖核酸酶保护试验及化学发光检测技术，研究了斜带石斑鱼GH分泌及其mRNA表达的调控机制，对于斜带石斑鱼的生长、繁殖、人工养殖等具有重要意义。

Orange-spotted grouper is a benthic protogynous marine fish with sex inversion. It is one of the most important culturing fish in the southeast of Asia. As in other vertebrates, its growth is mainly controlled by growth hormone . Few data are available concerning the regulation of GH in grouper. In this paper, the regulation of growth hormone release from pituitary of orange-spotted grouper and its mRNA level was determined by radioimmunoassay and RNase protection assay.

中文题名斜带石斑鱼生长激素分泌及其基因表达调控的研究副题名外文题名 Regulation of growth hormone and its gene expression in orange-spotted grouper，Epinephelus coioides 论文作者冉雪琴导师林浩然学科专业水生生物学研究领域\研究方向学位级别博士学位授予单位中山大学学位授予日期2003 论文页码总数140页关键词生长激素基因表达石斑鱼馆藏号BSLW /2003 /Q959 /39 应用分子克隆技术，构建了斜带石斑鱼生长激素(Growth hormone，GH)基因重组质粒，在大肠杆菌细胞中获得高效表达，亲和层析纯化斜带石斑鱼GH融合蛋白做为抗原，免疫家兔制备特异性抗体，通过酶联免疫实验、放射免疫测定、离体脑垂体静态孵育和液相杂交／核糖核酸酶保护试验及化学发光检测技术，研究了斜带石斑鱼GH分泌及其mRNA表达的调控机制，对于斜带石斑鱼的生长、繁殖、人工养殖等具有重要意义。

The successful cloning and expression of yak IL2 gene is putatively useful for the development of novel immunopotentiator to raise the immunity of yak against various infectious pathogens and the immuno-protective efficacy of vaccinations.

本实验成功克隆了犛牛IL2基因，其原核表达产物具有显著的免疫活性，这为研制新型免疫增强剂来提高犛牛对各种疾病的抵抗力，增强犛牛疫苗的免疫保护率奠定了基础。

To discuss mobile period rachitic and the relation with RAI, the author reachs humoral immunity function to have observation to the cellular immunity of 92 AR little patient, sum up an analysis to be as follows now. 1 object and method 1.1 objects rachitic little patient sets 92 mobile period for observation group, diagnose all accord with castigatory of group of coordination of scientific research of countrywide rachitic prevention and cure diagnosed a standard 1980. 92 in male 52, female 40, the age 3 months ~1 year old 48, 1~3 year old 39, 3 years old of above 5. 56 examine as a result normal children is set to contrast group, normal value 50%, among them male 34, female 22, the age 6 months ~3 year old.

为探索活动期佝偻病的免疫状态及和RAI的关系，笔者对92例AR患儿的细胞免疫及体液免疫功能进行观察，现总结分析如下。1对象及方法1.1对象92例活动期佝偻病患儿设为观察组，诊断均符合1980年全国佝偻病防治科研协作组修订的诊断标准。92例中男52例，女40例，年龄3个月~1岁48例，1~3岁39例，3岁以上5例。56例检验结果正常的儿童设为对照组，正常值50%，其中男34例，女22例，年龄6个月~3岁。

METHODS:The gene of human ET1 was synthesized according to the preferential codons of E. coli, cloned into the EcoRI and SalI sites of vector pThioHisA. The recombinant plasmid pThioHisA-ET1 was constructed , sequenced and transformed into E.coli TOP10. Induced and expressed fusion protein were identified and analysed by 12% SDS-PAGE and densitometry analyses. After the elution, denature and renature, the fusion protein Thioredoxin-ET1 was obtained by ProBondTM chromatogragraphy. The purity of Thioredoxin-ET1 was detected by HPLC. Inoculate Thioredoxin-ET1 once per mouse every 2 weeks in 25, 50 and 100μg separately on 3 groups for 4 times. 10 days after last inoculation, we obtained venipuncture blood.

根据人ET1的多肽序列合成ET1基因，将其插入到pThioHisA的EcoRI和 SalI位点，重组质粒pThioHisA-ET1进行酶切鉴定及序列测定验证后转化TOP10，IPTG诱导的重组菌经SDS-PAGE检测融合蛋白Thioredoxin-ET1的表达量；表达的融合蛋白用ProBond亲合层析纯化并经HPLC鉴测其纯度；每只小鼠按25、50、100ug/次剂量的Thioredoxin-ET1每两周免疫一次，共4次，最后一次免疫10d后制备抗血清，经Western blot和ELISA检测证明Thioredoxin-ET1融合蛋白具有ET1免疫反应原性。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。