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In organ transplantation, it has been shown that the anergic T cells may be generated in vivo and in vitro, and can be transferred as the tolerant cells to prevent allograft rejection.

研究母-胎免疫耐受机制,不仅对动物及人类自然流产的防治具有重要意义,而且对移植免疫和肿瘤免疫的研究亦将产生深远的影响。

Three different antiserum, rabbitantiserum against formalin-killed-cells of V. parahaemolyticus zj2003 (apathogenic strain isolated from diseased large yellow croaker, from Xiangshan bay,Zhejiang province), rabbit antiserum against OMP of the bacteria and large yellowcroaker convalescent antiserum were prepared for western blotting of the OMPpreparation.

以饱和硫酸铵梯度盐析法结合琼脂糖柱sephadex G200凝胶过滤法初步纯化了大黄鱼血清免疫球蛋白,再经割胶回收法得到进一步纯化的重链片段,分子量约为75kDa,免疫新西兰兔后获得兔抗大黄鱼免疫球蛋白血清,为ELISA和Western blotting检测技术奠定了基础。

Results ∶The erythrocytic immune functions of the recurrent aphthous ulcer were abnormal 、with very distinct significance.

介绍改善恢复免疫功能治疗方法,使用西药免疫促进剂及相当西药免疫促进剂的补肾药。

In terms of immunology, uterus is a special reproductive and immune organ. It shows immunological tolerance and immunological rejection respectively at the time of blastocyte implantation and parturition; blastocyte implantation is also concerned with the action of many cytokines and MMPs.

免疫学角度来讲,母体子宫作为特殊的生殖免疫器官在胚泡着床和分娩时分别表现为免疫耐受和免疫排斥,这两个生理反转过程必然涉及基因的时空差别表达;胚泡植入又涉及大量细胞因子及基质金属蛋白酶的协同参与。

Methods: Agar gel immunodiffusion test was conducted twice in agar gel plate with 0.1 mg/L bromophenol blue, first, agar gel immunodiffusion test was carried out with AFP-HSP70 cross-linking protein and AFP antibody, then the test was conducted again with the processed precipitation band and HSP70 antibody.

在含0.1 mg/L溴酚蓝的凝胶琼脂板上,进行2次琼脂免疫扩散实验,第1次是用AFP-HSP70交联蛋白和AFP抗体进行琼脂免疫扩散,把扩散后沉淀带处理后再次和HSP70抗体进行琼脂免疫扩散。

The glomeruli structure of antennal lobe is not classical. Accordingly, the mushroom body calyces have meager, and the lobes are relatively simple either. The central complex is prominent. The beetle showed notable 5-HT-like immunoreactivity throughout the whole brain, which was also strongly labelled by TPH antibody, with the pattern matching that of 5-HT. 5-HT-like positive fibers were resolved in all areas of the brain, among which the lamina and suboesophageal ganglion showed the most remarkable dark brown immunostaining.

免疫组化结果显示,异色瓢虫脑系统呈强5-HT免疫反应,表现为稳定的反应模式;其脑系统对鼠抗TPH单克隆抗体同样呈显著的免疫阳性反应,且反应模式与5-HT一致。5-HT能神经元多为切向神经元,数目相对较少,呈两侧对称分布,主要位于视叶与侧前脑之间、前脑背方及咽下神经节处,其阳性纤维呈明显的静脉曲张状,投射区域较广泛。

The recombinant proteins were purified according to the protocol of Ni-NTA Agarose and detected by SDS-PAGE, and TLC. Western blotting was used to identify their immunoreactivities with the serum of patient with clonorchiasis.

寄生性蠕虫以胞质GSTs为主,参与对由虫体代谢和宿主免疫系统所产生的脂质过氧化物和细胞毒性的羰基的解毒,对虫体的生存起重要的作用,是免疫疗法、化学疗法和免疫诊断潜在的靶标。

Primary study showed that body weight of broilers infected with ALV-J or REV, compared with the control, was significantly depressed and antibody response to NDV vaccine was inhibited. In addition, these broilers were susceptible to bacterial agents, such as colibacillosis, causing higher lethality rate in early time.

初步研究表明:单独感染和混合感染均可引起感染鸡生长发育不良,中枢免疫器官萎缩,免疫器官指数下降,对特定疫苗免疫应答降低,同时感染鸡早期对细菌性疾病的易感性增加,死亡率升高。

Based on these previous work, in this research, in order to explore the the molecular mechanism of enhanced tumor cell immunogenicity treated by elemene and mytomycin C compositely, we used murine Hca-F ascitic hepatic carcinoma ( high lymphatic metastasis substrain of H22) as an experimental model to compare the immunoprotective effects on Hca-F cells as well as the mechanism of the effects caused by HSP70-peptide complex purified from untreated tumor cells, EC-TCV and BCG; We studied the mechanism of increased tumor cell membrane HSP expression treated with elemene by the techniques of RT-PCR and immunoelectron microscopy; In order to deeply elucidate the antitumor effects and the molecular mechanism of elemene in increasing tumor cell immunogenicity we analyzed and compared the effects of elemene and heat shock on the gene expressions of tumor cell using gene expression profile chip.

本论文在上述研究成果的基础上,选择小鼠肝癌H22的高淋巴道转移亚系Hca-F为模型,进一步比较了未处理肿瘤细胞来源、榄香烯复合瘤苗来源的和卡介苗来源的HSP70-肽复合物对Hca-F的免疫保护效应、效应机制,探讨榄香烯-丝裂霉素复合处理增强肿瘤细胞免疫原性的分子机制;以Hca-F和人肝癌细胞株HepG〓等为模型研究了榄香烯处理增强肿瘤细胞膜热休克蛋白表达的机制;以HepG〓为模型用表达谱基因芯片研究比较了榄香烯与热休克处理对肿瘤细胞基因表达的影响,从而进一步阐明榄香烯抗肿瘤作用及增强肿瘤细胞免疫原性的分子机制。

Based on conal selection principle and K-Nearest Neighbor method, a fault detection algorithm that can produce the valid detector to diagnose fault is designed by the three parts of immune vaccine, immune learning and immune response.

3基于克隆选择原理和K-Nearest Neighbor分类法按照免疫疫苗、免疫学习和免疫应答三个过程设计了能生成有效检测器的故障检测算法。

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