免疫

Rhesus macaque is the best model to study of human immunodeficiency virus infection and to develop acquired immunodeficiency syndrome vaccine.

恒河猴是研究人免疫缺陷型病毒感染与获得性免疫缺失综合征疫苗研制的最为重要的动物模型，其组织相容性复合体结构的解析将有助于了解HIV 免疫逃逸机制。

guguan capsule has a definite effect on patients with metrorrhagia caused by intrauterine device , and the possible machenism is that guhuan capsule can improve body immune system.

其作用机制可能是通过提高红细胞对机体的免疫调节作用，清除机体中免疫复合物，降低ricr、sil 2r水平，改善机体免疫功能的结果。

Scanning electron micrography and X-ray fluorescence spectrometry were employed to characterize the construction process of the immunosensor. Cyclic voltammetry and differential pulse voltammetry were used to study its electrochemical properties. The content of CAP was determined with competitive immunoassay.

采用扫描电镜、X射线荧光光谱表征了免疫传感器的制备过程，采用循环伏安法和示差脉冲伏安法研究了免疫传感器的电化学性质，结合竞争性免疫分析法测定了CAP浓度。

Significant Spearman rank correlations were found between the results obtained by Luminex assay and by chemiluminescence immunoassay or time-resolved fluorescence immunoassay. Only 1 microliter of serum and 3 hours were needed for Luminex assay. With the advantages of unique capability of multiplexing and high-throughput, good reproducibility, increased detection range and sensitivity, Luminex assay would have a great potential in clinical application.

本研究应用液相芯片技术利用双抗体夹心免疫分析原理同时检测了人血清中的癌胚抗原、甲胎蛋白和乙肝表面抗原，评价了该方法的线性范围、最低检测限、精密度、灵敏度、特异度、准确度等指标，并对检测结果与化学发光免疫分析法（chemiluminescence immunoassay，CLIA）和时间分辨荧光免疫分析法（time-resolved fluorescence immunoassay，TRFIA）进行了相关性分析。

Methods Using prime-boost immunization strategy,BALB/c mice were inoculated muscularly with HIV-2 gp105 recombinant DNA vaccine and fowlpox virus.

目的：探讨应用prime-boost免疫策略进行HIV-2外膜蛋白重组鸡痘病毒与重组DNA疫苗联合免疫引起小鼠的免疫应答。

In order to develop a safe and effective immunoadjuvant to enhance the immunity and resistance of animals against infection, a novel CpG Oligodeoxynucleotides containing 11 CpG motifs was synthesized and inserted into the VR1012 plasmid, designated as VR1C. Then the recombinant VR1C was entrapped with Chitosan nanoparticles prepared by the method of ionic cross linkage, and employed to inject muscularly 3-weeks old Kunming mice; the blank VR1012 packed with CNP and saline were used to inoculate mice as the control groups. 28 days after inoculation, all mice were orally fed with 0.4ml 2x108CFU/ per mouse virulent hemorrhagic enteritis E. coli to challenge the resistance against infection.

为研制安全高效免疫调节剂增强动物免疫抗病能力，本实验设计合成含11个C pG基序的寡聚核苷酸，重组构建含CpG的VR1012质粒(VR1C)；制备壳聚糖纳米颗粒包裹重组质粒(CNP-VR1C)，肌注接种3周龄昆明小白鼠，设壳聚糖包裹空质粒和生理盐水对照组；接种后28天口服大肠杆菌攻毒观察小鼠天然免疫的变化和对强毒感染的抵抗力，Sandwich ELISA测定血清免疫球蛋白和白细胞介素含量。

Some serum samples from ducks immunized with attenuated DPV were assayed with the ELISA kit, and the antibodies were detected first on day 6 or day 9 post-immunization in ducks vaccinated subcutaneously, orally or nasally, and still detectable on day 60 PI.

应用该试剂盒对皮下注射、口服和滴鼻免疫DPV弱毒的20日龄樱桃谷鸭血液中抗DPV特异性IgG抗体效价进行了测定，皮下免疫鸭于第6d，口服和滴鼻免疫鸭于第9d可在血清中检测到DPV特异性抗体，且至第60d时依然可检测到高效价抗体。

Results ①Compared to sham group, ISH and IHC of rat hypothalamic CRHshow positive cells numbers increase, positive areas enlarge and average gray scaledecreases, which reveals the expression of hypothalamic CRH neurons enhances afterPX;②The enhancement of ISH and IHC of hypothalamic CRH in PX rats aftermelatonin supplementation dose-dependently recovers, but it can't return to be normaleither in high dose group or in low dose group and either at 4th week or at 8th week;③IHC of CRH in median eminence of neurohypophysis shows positive areas enlargeand average gray scale decreases, which reveals the releasing of CRH fromhypothalamic CRH neurons increases;④ELISA of CRH in serum shows thedifferences of CRH concentrations among all groups are not significant, which indicates PX and melatonin supplementation have no significant effect on CRH levels.

结果： ①松果体摘除后大鼠下丘脑室旁核CRH 原位杂交和免疫组化的结果与假手术对照组相比均显示阳性细胞数增多、阳性面积增加、平均灰度降低，提示松果体摘除后下丘脑CRH 神经元的表达增强；②松果体摘除大鼠补充褪黑素后，下丘脑CRH 原位杂交和免疫组化染色增强呈剂量依赖性恢复，不过不论是低剂量组还是高剂量组，也不论是术后4 周还是术后8周，均不能恢复至正常；③松果体摘除后神经垂体正中隆起CRH 免疫组化结果显示阳性面积增加、平均灰度降低，提示下丘脑CRH 神经元释放CRH 增加；④ELISA 法检测血清CRH结果示各组血清CRH 浓度差异没有显著性，表明松果体摘除及补充褪黑素对血清CRH 水平没有显著影响。

Results ①Compared to sham group, ISH and IHC of rat hypothalamic CRHshow positive cells numbers increase, positive areas enlarge and average gray scaledecreases, which reveals the expression of hypothalamic CRH neurons enhances afterPX;②The enhancement of ISH and IHC of hypothalamic CRH in PX rats aftermelatonin supplementation dose-dependently recovers, but it can't return to be normaleither in high dose group or in low dose group and either at 4th week or at 8th week;③IHC of CRH in median eminence of neurohypophysis shows positive areas enlargeand average gray scale decreases, which reveals the releasing of CRH fromhypothalamic CRH neurons increases;④ELISA of CRH in serum shows thedifferences of CRH concentrations among all groups are not significant, whichindicates PX and melatonin supplementation have no significant effect on CRH levels.

结果： ①松果体摘除后大鼠下丘脑室旁核CRH 原位杂交和免疫组化的结果与假手术对照组相比均显示阳性细胞数增多、阳性面积增加、平均灰度降低，提示松果体摘除后下丘脑CRH 神经元的表达增强；②松果体摘除大鼠补充褪黑素后，下丘脑CRH 原位杂交和免疫组化染色增强呈剂量依赖性恢复，不过不论是低剂量组还是高剂量组，也不论是术后4 周还是术后8周，均不能恢复至正常；③松果体摘除后神经垂体正中隆起CRH 免疫组化结果显示阳性面积增加、平均灰度降低，提示下丘脑CRH 神经元释放CRH 增加；④ELISA 法检测血清CRH结果示各组血清CRH 浓度差异没有显著性，表明松果体摘除及补充褪黑素对血清CRH 水平没有显著影响。

Before the first immune and 1 week after strengthened immune, take blood and measure AsAb with ELISA method, measure the immune integral of IgG and IgA in uterus tunica intima with SABC method; meanwhile, compare the pregnant rats and average nidation points in all groups.

在首次免疫前、各次加强免疫后1周取血ELISA法测定AsAb，SABC法检测子宫内膜IgG、IgA免疫积分，同时比较各组大鼠受孕数及孕鼠平均着床点数。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。