免疫

Theoretically, its calculation complexity is studied, while it is tested empirically by learning multi-samples generated randomly. The comparative analysis and applications show it has some merits of rapid searching and good clustering effect. B3. An immune network optimization algorithm with information feedback, which can solve special complex function optimization problems with high dimensions which objective functions are formed by summing multi-subobjective ones, is constructed based on the clone selection principle and the immune network regulation of the idiotype immune network.

为了对免疫系统中抗体如何学习抗原特征的整体行为有一定的认识，从单个抗体识别抗原及多个抗体并行识别抗原的特征出发，利用免疫应答的相关原理及免疫细胞的免疫功能设计了适合于批量样本聚类的抗原特征学习算法，理论上分析了其计算复杂度，获得了此算法中的关键参数与其搜索性能的关系；应用上，该算法通过对实际的多样本学习，获得了搜索速度快及聚类效果好的优点，比较分析表明这种方法是可行的和有效的。

Syngenic monoclonal anti-idiotypic antibodies (MAb_2s), AFE_8Id_(16-6) and ALA_(15-6)Id, were raised against an idiotype on a protective monoclonal antibody with specificity for NDV.

用AFE_8Id_(16-6)和ALA_(15-6)Id两株新城疫病毒的抗独特型单抗(MAb_2)分别免疫接种一组(18只)无HI抗体鸡，并以一组(7只)Lasota苗常规免疫鸡和一组(12只)不免疫的易感鸡分别作为免疫阳性和阴性对照。

Conclusions】 It is suggested intramuscular injection is an effective immune route. Mice inoculated with coding different stage gene recombinants alone or mixedly could all induce increased humoral and cellular immune response, and NK cell activity.

提示肌肉注射为一有效的DNA疫苗免疫途径，采用编码恶性疟原虫有性期阶段或无性红细胞阶段的重组质粒单独或两者混合免疫小鼠，均能诱导明显的体液免疫反应、细胞免疫反应和NK细胞杀伤活性。

By oral adminstration of methanol extract of Hoshouwu, the cytotoxic activity of natural killer cells and myelopoiesis were enhanced.

免疫调节方面，进行口服何首乌成分对免疫系统调节作用的动物实验研究，包括免疫细胞活性及抗体免疫反应方面的观察。

Healthy rabbits were inoculated each with vaccines of the recombinant Pilin and the recombinant fused Pilinn-IL2 protein at 250ug per dose to potentiate the immunogenicity. The D. nodosus Pilin vaccine and the killed D. nodosus whole cell vaccine were also inoculated into rabbits as controls in order to compare with the above two recombinant vaccines.

用纤毛蛋白基因工程疫苗、Pilin-IL-2融合基因工程疫苗分别免疫健康家兔，初步进行了纤毛蛋白疫苗和Pilin-IL-2融合基因工程疫苗的免疫原性试验，并同时用节瘤拟杆菌菌体灭活疫苗及菌体纤毛蛋白疫苗免疫家兔，进行了免疫原性比较试验。

By comparing the body weight, immune organs index and response of lymphocytes to ConA, to analyze the effect of QKL on immunological system of normal mouse. Delayed type hypersensitivity mouse model was established by stimulation with dinitrofluorobenzene. By comparing spleen index and swelling rate of pathogenetic ear, to illuminate the immunoregulation of QKL in vivo. Polymicrobial sepsis mouse model was induced by cecal ligation and puncture. The cumulative survival rate was observed in QKL and NS groups. Results: QKL had no influence on body weight and spleen index of normal mouse, but it could significantly decrease thymus index and inhibit the immune response of lymphocytes to ConA.

连续皮下注射QKL 5 d，通过检测小鼠体重、免疫器官指数和淋巴细胞对ConA刺激的反应，分析QKL对正常小鼠免疫功能的影响；采用二硝基氟苯诱发小鼠耳朵肿胀，发生接触性皮炎，建立DTH模型，通过比较QKL与NS对照对小鼠脾脏指数和发病耳肿胀抑制率评价QKL体内对小鼠的免疫调节功能；采用盲肠结扎穿孔术后引起的多细菌性腹膜炎，建立小鼠脓毒症模型，通过观察QKL对小鼠存活状况的影响，评价QKL的免疫调节作用。

①Rat MSC and VSMC were cultured and identified, respectively. MSC were labeled with DAPI firstly, and then co-cultivated with VSMC. The changes of morphology and ultrastructure of co-cultured cells were observed. Immunfluorescence analysis was performed by using monoclonal antibodies against specific antigen.②We established the regulatable system in two steps: a stable MSC line expressing rtTA has been constructed and characterized firstly by transfected with pUHD 17-1hyg and then selected by hygromycin B; in a second step, this line was used for trandfer the AT2R gene to MSC to get the well establishing double stable MSC lines;③The expression of AT2R regulated by doxycycline was evaluated by western blot;④The MSCs were transduced into rat carotid arteries with regulatable AT2R gene after the establishment of rat carotid balloon injury restenosis model. The intimal/medial area ratio were measured by digital analysis system.

研究方法：(1)密度梯度离心法及胶原酶消化法分别培养原代大鼠MSC及VSMC，细胞共培养并行免疫荧光化学染色和透射电镜观察超微结构；(2)组成受Dox调控的哺乳动物表达系统的四种成分的转化、扩增及提纯并酶切鉴定；(3)采用常规分子生物学方法连续两个回合转染体外培养的MSC，并分别采用发光计检测不同细胞克隆萤光素酶活性改变以及RT-PCR方法检测AT2R目的基因mRNA表达情况，根据各个细胞克隆受Dox调控表达的程度，选择低背景、高诱导表达AT2R的细胞系，作为双重稳定MSC细胞系；蛋白免疫印迹法观察该细胞系在Dox调控下AT2R表达的时相性、持续性及在不同浓度Dox调控下的表达情况；(4)建立大鼠颈动脉球囊损伤动物模型，将双重稳定MSC在术中导入血管，分别于14 d、28 d进行病理切片，检测可调控AT2R对新生内膜增生的影响；采用RT-PCR免疫组织化学免疫荧光等技术观察AT2R基因在新生内膜中的表达以及细胞外基质成分表达的改变，TUNEL法检测血管组织中细胞凋亡的变化情况。

Methods Red cell immune adherence function,T-cell and its subsets in peripheral blood,immunoglobulin and complement in serum were detected by erythrocyte saccharomycete rosette test,indirect immune fluorescence staining and flow cytometry and radio immuno-dif...

方法应用红细胞酵母菌花环试验、间接免疫荧光流式细胞仪检测法、单向免疫扩散法对围产期窒息及HIE患儿红细胞免疫粘附功能，血T细胞亚群、血清免疫球蛋白及补体进行检测。

Plaster Yiyi can modulate humoral immunity and cell-mediated immunity, solidate the recovery and protection function, especially for the soft tissue hurt and low-immune function condition.

乙乙膏能调节体液免疫和细胞免疫(Cellular cell-mediated immunity)状态，增强机体对免疫器官的保护和恢复作用，对软组织损伤时，机体的免疫功能低下有良好的改善作用。

There were no significant differences for the firing rates in the site of contralateral TNC neurons among during pre-CSD,CSD,and post-CSD (P＞0.05).For flunarizine group,the firing rates in the site of ipsilateral TNC neurons during pre-CSD were higher as compared with during CSD(P＜0.05).2.1 There were statistical differences on palasma levels of CGRP and SP among the three groups(P＜0.05).The levels of CGRP and SP in CSD group were higher than control group(P＜0.05).No significant differences on the levels of CGRP and SP in ipsilateral trigeminal ganglia were found among the three groups(P＞0.05).2 The number of neurons with positive CGRP and SP immunoreactivity was statistically different in right-sided trigeminal ganglia among the three groups (P＜0.05).The number in fight-sided trigeminal ganglia in CSD group was higher as compared with control group(P＜0.05).The number in right-sided trigeminal ganglia was statistically higher than that in left-sided trigeminal ganglion in CSD group(P＜0.05).3.1 Altered ReHo in ipsilateral pons and other brain regions response to pain such as basal nuclei,thalamus,cingulated gyms and prefrontal cortex was detected during the acute spontaneous attack as compared with during headache remission(P＜0.05,corrected by Monte Carlo simulation). 2 Positive functional connectivity was detected between ipsilateral pons and other brain regions related to pain within pain state and within non-pain state (P＜0.05,corrected by false discovery rate,FDR).Increased functional correlation between ipsilateral pons and other pain-related brain regions such as ipsilateral prefrontal cortex and contralateral subcallosal gyrus was detected during the acute spontaneous attack as compared with during headache remission(P＜0.05,corrected by Monte Carlo simulation).

结果1。对照组未发现CSD；同侧TNC放电频率，CSD中＞CSD后＞CSD前P＜0.05对侧TNC放电频率，CSD前、中、后无统计学差异(P＞0.05氟桂利嗪组同侧TNC放电频率，CSD前＞CSD中(P＜0.05)，CSD前与CSD后及CSD中与CSD后之间无统计学差异(P＞0.05)。2.1关于放免测定，各组血浆CGRP、SP水平有统计学差异(P＜0.05)，CSD组高于对照组(P＜0.05)，CSD组与氟桂利嗪组、对照组与氟桂利嗪组之间均无统计学差异P＞0.05各组之间同侧三叉神经节中CGRP、SP水平未见变化(P＞0.05.2关于免疫组化研究，右侧三叉神经节CGRP、SP免疫阳性细胞数三组之间有统计学差异(P＜0.05)，多重两两比较结果CSD组大于对照组(P＜0.05)，CSD组与氟桂利嗪组之间、对照组与氟桂利嗪组之间无统计学差异P＞0.05左侧三叉神经节CGRP、SP免疫阳性细胞数三组之间无统计学差异(P＞0.05CSD组中右侧三叉神经节CGRP、SP免疫反应阳性细胞数大于左侧(P＜0.05)。3.1局部一致性分析发现两组患者头痛疼痛状态较非疼痛状态脑活动发生变化的脑区有同侧脑桥以及其他疼痛相关脑区如基底节区、丘脑、扣带回、前额叶皮层等(P＜0.05，蒙特卡罗模拟校正)。2功能连接分析发现疼痛状态与非疼痛状态下主要疼痛相关脑区均与同侧脑桥有功能联系P＜0.05，false discovery rate，FDR校正疼痛状态与非疼痛状态比较，同侧前额叶皮层、对侧胼胝下回等疼痛相关脑区与同侧脑桥之间功能联系增强(P＜0.05，蒙特卡罗模拟校正。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷