克隆

We have cloned gene, designated as PvfadA, for C18:0 Δ9 desaturase catalytic activity from Pavlova viridis by RT-PCR, RNA ligase mediated RACE and Overlap-PCR strategy.

我们通过RT-PCR、RNA ligase mediated RACE and Overlap-PCR方法从海洋微藻绿色巴夫藻中克隆到一个命名为PvfadA的脂肪酸去饱和酶候选基因。

A pair of oligonucleotide primers were designed according to the conserved region of MC1R gene of gallus, coturnix japonica, anser caerulescens, and pavo cristatus.

本研究根据鸡、鹌鹑、雪雁、孔雀等MC1R基因同源序列的保守区设计1对引物，以半番鸭、北京鸭总DNA 为模板，PCR扩增，克隆测序。

Semiquantitative PCR analysis revealed that the levels of pectate lylase and β-galactosidase gene (MA-gal1) transcript were related with the banana fruit ripening and softening.

通过半定量PCR分析表明，所克隆的β-半乳糖苷酶基因（MA-gal1）和果胶裂解酶基因的表达与果实成熟软化相关。

With genomic DNA of Clostridium stercorarium as a template, a structural gene pel9A encoding pectate lyase was obtained by PCR amplification. The pel9A was cloned into pET28a to recombine an express plasmid pPel9A, and then the plasmid pPel9A was transformed into E. coli BL-21, expressed under the control of T7lac promoter at 37℃.

利用PCR技术从耐热梭状芽孢杆菌中扩增得到产耐热果胶裂解酶的结构基因pel9A，并将其克隆于表达载体pET28a中，最后将此重组质粒转化到受体菌E.coli BL-21中进行表达。

Then, cloning would be permissible in a small range of cases.

那么，克隆可能会在小范围的案例中被许可。

77%(35/45) of positive clones from eluted phage clones were obtained by 3 rounds of biopanning.

经过3轮淘洗，获得的阳性克隆率为77%(35/45)。

Inan attempt to select more efficient biocatalysts ,the hydantoinase and carbamylase genes fromBurkholderia pickettii were cloned in Escherichia coli, achieve a co-experssed strains. The geneswere assembled to give the carbamylase gene preceding the hydantoinase gene. The recombinantstrains stably and constitutively produced the two enzymes and efficiently converted thecorresponding hydantoins onto p-hydroxyphenylglycine and phenylglycine .

我们试着寻找更快更有效的转化菌株，把来源于皮氏伯克霍尔德氏菌的表达该两种酶的两个基因同时克隆到大肠杆菌中，获得共表达两种酶的基因工程菌株，两个基因的顺序是 Dcase 基因在 Dhase 基因的前面，同一个启动子。

Locations of transgenes in the recovered plasmids were determined by southern blotting. And then adjacent sequences of transgenes integration sites in three recovered aberrant classes were subcloned and analyzed. Transgene of A-2 was inserted into common carp DNA sequences homologous to the mouse phosphoglycerate kinase-1 gene and those of A-3 and A-6 were inserted into common carp DNA sequences homologous to the human epidermal keratin 14 gene and sequence of common carp β-actin gene, respectively.

通过Southern杂交对转植基因在回收质粒上的位置进行了定位分析，并对三种回收到的变异型转植基因旁侧顺序进行了亚克隆和顺序测定。A-2的转植基因整合到了与小鼠磷酸甘油酸盐激酶-1基因同源的鲤鱼基因组顺序旁侧，A-3的转植基因整合到了与人表皮角蛋白14基因同源的鲤鱼基因组顺序旁侧，A-6的转植基因整合到了普通鲤鱼β-actin基因顺序中。

Human cytomegalovirus ; Phosphoprotein ; Molecular cloning ; Restriction site

人巨细胞病毒；磷蛋白；分子克隆；限制性酶切位点

Cloning and sequencing of the phycocyanin gene from Spirulina maxima and its evolutionary analysis.

极大螺旋藻藻蓝蛋白基因的克隆及其进化分析。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。