克隆

In this study, we investigated the gene expression pattern of Cbfal in odontoblast as well as tooth development by gene cloning and in situ hybridization. The results revealed the essential role cbfal plays in tooth development.

本研究采用基因克隆与原位杂交的方法，研究了cbfal在牙乳头细胞及牙胚发育中的表达，初步证实了cbfal在牙胚发育中的作用，同时，作为整体实验设计的一部分，本研究所构建的cbfal cDNA探针及反义核酸cbfal真核表达载体为对cbfal进一步的研究奠定了基础。

Moreover, the pattern of odorant receptor gene expression and the organization of odorant receptor genes in cloned mice was indistinguishable from wild-type animals, indicating that irreversible changes to the DNA of olfactory neurons do not accompany receptor gene choice.

过去，克隆的动物一直是用来自分裂细胞的DNA培育的，如皮肤细胞。新的结果表明，来自非分裂的、成熟的细胞的DNA可被重新编排，表达正常发育所需的一套完整的基因。

In this paper,techniques of reverse transcription polymerase chain reaction and rapid amplification of cDNA ends have been used to clone a gene,which encodes a general odorant binding protein,from the antennae of Loxostege sticticalis.

利用RT-PCR结合RACE技术克隆了编码草地螟触角中一种普通气味结合蛋白的基因。

There are more than the Internet and Acorn International, the East's best known companies, such as similar to the shopping company, most of the names of only一字之差, is more direct cloning site. 2009 CCTV'3 15 'TV show on the crook of the exposure to the company, after a few days in the quiet resurgence of the company liar.

互联网上有多家与橡果国际、东方购物等知名公司雷同的购物公司，大多名字仅一字之差，更有甚者直接克隆网站。2009年央视'3.15'晚会对骗子电视购物公司进行了曝光，在沉寂几天之后骗子公司又死灰复燃。

In our experiment, the GADes gene from rat brain was cloned and expressed in E.coli, so as to make the recombinant GADes produced by gene technology for clinical application and further study on the relation between GAD and type 1 diabetes mellitus.Firstly, with the pUClS/GADes recombinant plasmid as template, the full-length encoding sequence of GADes was amplified by PCR, and cloned into pGEM-T vector. Secondly, the target gene was cut by EcoRI and inserted into restriction sites of pET42a vector for expression.

本研究首先用PCR方法，以pUC18/GAD_(65)为模板，扩增目的基因，克隆到pGEM-T载体中，构建pGEM-T/GAD_(65)重组质粒，然后用EcoRI酶切，切下的目的基因片段插入pET42a融合型表达载体中，经酶切鉴定和测序，证实插入方向、读码框架正确；重组表达质粒转化大肠杆菌BL21(DE_3)中诱导表达，表达产物经亲和层析纯化后获得了融合蛋白GST-GAD_(65)。

Two genes which were differentially expressed before and after ATRA treatment have been partly cloned, and one corresponds to a sequence known gene DPH2L with unknown function, which was first isolated from the critical region of deletion on chromosome 17p13.3 in human ovarian carcinoma, and regarded as a candidate tumor suppressor gene.

它的降调节可能参与了从细胞生长到分化／凋亡的转换过程。另一个克隆RAa7是一个未曾报道的新序列，在ATRA或4HPR反应性细胞中呈上升或下降调节，提示该基因可能也参与了细胞过程转换的调节。

CLONALG emphasizes that ontogenetic adaptive changes can be achieved by cumulative blind variation and selection within organisms.

基于以上机理，笔者认为在设计克隆选择算法时应该把基因重排考虑进去。

SO7 gene of Eizneria tenella YZ strain was amplified by RT-PCR from total RNA of sporulating oocyst, cloned into pUCm-T, and then sequenced.

通过RT—PCR扩增柔嫩艾美球虫YZ株折光体SO7抗原基因，并进行克隆和测序。

Methods The total RNA was extracted from the brain of human embryo. The segment of open read frame of DOC-1 was amplified by RT-PCR and was further cloned into the vector, pMD18-T, and the prokaryotic expression vector, pGEX-4T-1, by turns, to produce the new construct pGEX-4T-1-DOC-1. After restriction enzymes digestion analysis and sequenced, pGEX-4T-1-DOC-1 was transformed into E.coli BL21（DE3）. GST-p12 recombinant protein was expressed under IPTG induction and purified by affinity column.

从人胎脑组织中提取总RNA，经逆转录-聚合酶链式反应扩增DOC-1的CDS序列，再通过基因重组技术将该基因片段依次克隆到pMD18-T和pGEX-4T-1载体中，构建融合表达载体pGEX-4T-1-DOC-1，经酶切、测序鉴定后，用该重组质粒转化E.coliBL21，用IPTG诱导表达，Glutathione Sepharose 4B柱亲和层析纯化重组蛋白。

This is an immune clone algorithm procedure for solving a nonlinear problem of the optimal value function.

详细说明：这是一个免疫克隆算法程序，求解了一个非线性的函数问题的最优值。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。