克隆

The new chimeric infectious DNA clone and its derived, a virulent chimeric virus are constructed from the nonpathogenic PCV1 in which the immunogenic ORF gene of the pathogenic PCV2 replaces a gene of the nonpathogenic PCV1, preferably in the same position.

所述新型嵌合的传染性DNA克隆及其衍生的有毒力的嵌合病毒是用非致病性PCV1构建的，其中，致病性PCV2的免疫原性ORF基因取代了非致病性PCV1的基因，优选是在相同的位置上取代。

In this paper, the complete nucleotide sequence of watermelon mosaic virus Chinese Isolate was determined. On the basis, the HC-Pro protein and CP protein both are the factors for aphid transmission were cloned and expressed in vitro, and their antiserum were prepared. By far western blot, viral receptors were identified in aphids, and their interactions were studied. The molecular mechanism of nonpersistent transmission was further elucidated.

本文完成了我国西瓜花叶病毒的基因组全序列的测定，在此基础上，克隆和体外表达了蚜传因子HC-Pro和CP蛋白，制备了相应抗体，利用Far western blot技术鉴定了其蚜虫体内的受体蛋白，并对三者互作关系进行研究，进一步阐明了非持久性传毒的分子机制。

By adding new self or nonself to meet system need dynamically, and standing a clonal selection additionally in the system after adding new self or nonself, some detectors arriving to threshold can be activated, others are to die or wait to be activated.

通过新增自体或非自体来动态满足检测系统的要求，并在系统中再一次进行克隆选择，达到激活阀值的检测器才能被激活，而没有达到的或死亡，或等待被激活。

Now that I'm out of the organization, I no longer have access to a licensed copy of Norton Ghost.

现在，我来到了该组织的，我已经不再使用的许可副本诺顿克隆精灵。

Cloning could massively improve the agricultural industry as the technique of nuclear transfer improves.

随着核转移技术的提高，克隆能大规模改善农业工业。

The major factors affected the efficiency of goat embryo nuclear transfer technique were discussed systematically in this study in order to establish an acceptable manipulation and production system of embryo nuclear transfer technique in our lab.

本研究系统的探讨了山羊胚胎细胞核移植过程中的几个主要影响因素，以期完善山羊胚胎细胞核移植操作程序和胚胎细胞核克隆胚胎生产体系。

Cloning, or nuclear transfer, is a laboratory technique which gets around the need for conventional fertilisation, the union of sperm and egg which brings together two half-sets of genetic material to provide the resulting individual with a full complement of chromosomes.

克隆，或称细胞核转移，是一种实验室技术，它绕过传统的繁殖方式，即精卵结合的受精方式，后者是把两套各一半的遗传物质合在一起构成新个体的完整染色体。

Alkaline nuclease gene and its flanking region of Bombyx mori nucleopolyhedrovirus strain Suzhou was cloned, sequenced, and analyzed.

基于为杆状病毒分类提供新的依据，克隆了家蚕核型多角体病毒苏州株碱性核酸酶基因及其侧翼区，并进行序列和进化分析。

The nucleoprotein gene and glycoprotein gene of rabies virus were amplified by RT-PCR and then cloned into pMD18-T plasmid,respectively.

将N基因和G基因分别克隆入质粒载体pMD18-T后，对筛选的含有N基因和G基因的重组质粒进行了全基因序列测定和分析。

Objective To stusy the cloning of the genes of glycoprotein and nucleoprotein of rabies virus and expression of the recombinant protein G and N in insect cells by using the baculovirus-insect cell expression system.

目的 从国内狂犬病疫苗aG株中克隆狂犬病病毒糖蛋白基因和核蛋白基因，应用杆状病毒-昆虫细胞表达系统使其在昆虫细胞中表达。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。