克隆

Abstract］ Objective: To clone the defensin genes of Musca domestica larvae and analyze their sequences.

目的：克隆家蝇幼虫防御素基因并进行序列分析。

The cDNA encoding AChE from susceptible and propoxur-resistant strains of Musca domestica had been cloned using RT-PCR.

2利用RT-PCR技术克隆了家蝇敏感和残杀威抗性品系的乙酰胆碱酯酶基因。

The cDNA sequence encoding ubiquitin from Musca domestica was cloned by RT-PCR and sequenced.

本研究根据GenBank已登录的真核生物泛素编码框的氨基酸序列，设计一对简并引物，RT-PCR克隆了家蝇Musca domestica泛素基因的编码区cDNA序列，并进行了测序。

Muscovy duck reovirus ; NS gene ; cloning ; sequence analysis.

番鸭呼肠孤病毒； NS基因；克隆；序列分析

In order to compare the homology of the gene encoding VP2 between MDPV-Q and MDPV which was registered in the GenBank, also in order to find out changes of the VP2 gene and the immuno-genicity between the wild-strain MDPV-Q and the attenuated strain MDPV-26 which derived by continued passage of virulent wild-type in muscovy duck embryo, a pair of primer (LHMP7/LHMP8) was designed. The upper one LHMP7 and the lower one LHMP8 corresponded to the MDPV-specific nucleotides sequence 2885-2900 and 4618-4604 respectively, according to the MDPV nucleotide sequence databases. The length of the sequence embraced by the primers was 1734bp.

为了获取这一基因片段与国外分离株进行同源性比较，同时也为了了解MDPV强、弱毒株VP2基因之间的异同关系，及其免疫原性的变化规律，通过DNA重组技术，设计了一对引物LHMP7／LHMP8，该对引物选取位于2885～2900及4618～4604的两段序列，跨幅为1734bp，并在这两条引物中分别加入两种限制性核酸内切酶SacⅡ和KpnⅠ的酶切位点，分别对MDPV-Q和由该株病毒经人工致弱后得到的MDPV-26株进行PCR扩增，并将PCR产物克隆到pMD18-T载体上，分别得到二个重组子：pMD18-T—M-Q VP2和pMD18-T-M-26 VP2。

Three isoforms of myosin heavy chain fragments, two of which were the first time reported, were isolated.

克隆到三条对应于肌球蛋白重链尾部保守区的异构体，其中两条是首次报道。

NBAC plans to call for a continued ban on federal government funding for any attempt to clone body cell nuclei n.

NBAC计划呼吁继续禁止使用联邦政府基金利用人体细胞核去克隆婴儿的任何企图。

Methods: The expression of EOMES gene, ETS-1 gene and MEF (myeloid Elf-1 like factor) gene in 25 cases of N-NK/T-L and 25 control cases including 11 cases of B cell lymphoma (BCL, 6 cases of T cell lymphoma, 3 cases of normal spleen, 5 cases of chronic nasopharyngitis were detected by tissue microarray and in situ hybridization.

通过对EOMES，ETS-1和MEF基因进行克隆、探针制备和组织芯片制备，对25例N-NK/T-L以及同部位11例B细胞淋巴瘤、6例T细胞淋巴瘤、3例正常脾组织和5例慢性炎性鼻黏膜组织进行了原位杂交检测。

Experiments were performed in three groups of mice,20 minutes later ,mice in control group were treated with 2ml PBS by ultrasonic nebulization for 20 minutes, mice in treatment group were treated with rhIL-10(10ng) by ultrasonic nebulization for 20 minutes.

结果：经DNA测序证实，克隆的hIL-10cDNA与文献报道的一致，应用ELISA方法检测证明在大肠杆菌中成功表达出了hIL-10，含量＞400pg/ml，检测其生物学活性与标准品无明显差异。

By using PCR method,partial sequences of chs gene exon2 were amplified from cDNA of Nelumbo /Jwci/era.

膨胀素基因的克隆膨胀素是一类存在于植物细胞壁上，并能缓冲细胞壁结构网中的张

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐