克隆

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报酬由其余四个子公司分摊。3、对克隆技术的经济规模进行控制，如一次只能控制20个。4、其他笔者考虑不到的内容，如建议克隆已经破译遗传密码的人种。

According to the difficulties of detecting Clone Trusted Platform Module in DAA protocol using variable basename, we propose two new methods to detect Clone TPMs, which offers TPMs high degree of anonymity. Moreover, in order to reduce the time cost of verifying the DAA signature, a new multi-signature scheme is proposed.

针对DAA协议中使用可变名字基时检测克隆可信平台模块(Trusted Platform Mudule，TPM)存在的困难，在保持TPM高匿名度的前提下，提出了两种新的克隆TPM检测方案；针对验证DAA签名的时间开销过高的问题提出了一种多重签名方案。

It can be concluded that clonal selection algorithm is convergent, because BCA is an abstract of the generic CSA.

由于BCA是一般克隆选择算法的一种抽象，因此可以推断克隆选择算法的收敛性。

To some people, the question to clone or not to clone, in a sense, could be comparable to the question to be or not to be.

对某些人来说，克隆不克隆的问题在某种意义上可以比作是生还是死的问题。

We optimized reaction system for labelling-antibody in which the optimal amount of the purified anti-Stx2B IgG conjugated with colloidal gold beads was 60μg/mL, the purified antibody for E.coli O157 was 57μg/mL, the optimal pH was 8.2, the size of colloidal gold partical was 20nm, the optimization of stabilizing agent was BSA, the optimization buffer was boracic acid buffer with pH 8.2, the optimization of preserving fluid and eluant was boracic acid buffer with pH 8.2 including 5mM NaCl-1%BSA, confining liquid for NC membrane was 0.01% PBST with 3%BSA, the amount of polyclonal antibody against E.coli O157 and Stx2 conjugated with colloidal gold beads for conjugate pad was 3μg respectively, the amount of anti-Stx monoclonal antibody for test line was 0.1μg and 1μg for E.coli O157, the amount of goat anti rabbit IgG for control line of both GICA were 1μg.

测定了胶体金颗粒的最优化反应体系：胶体金颗粒的大小为20nm；抗体与胶体金溶液结合的最佳pH约为8.2；最佳蛋白结合量分别为抗大肠杆菌O157 IgG为57μg/mL，抗重组Stx2B IgG为60μg/mL；最佳稳定剂为BSA；最佳缓冲液为pH8.2硼酸溶液；最佳金标保存液和洗涤液为5mM NaCl-1%BSA的pH 8.2的硼酸缓冲液；NC膜的封闭液为3%BSA的0.01mol/L PBST；Stx2试纸条和大肠杆菌O157试纸条的质控线上的羊抗兔IgG的多克隆抗体最佳点样量均为1μg，其检测线的抗Stx2的单克隆抗体的点样量为0.1μg、抗大肠杆菌O157的单克隆抗体的点样量为1μg，结合垫上的金标抗大肠杆菌O157和重组Stx2B多克隆抗体点样量均为3μg。

Results The cell size appeared uniform,cell boundary was distinct,the cell recovery rate after frozen storing was above 90%.

出于对该突变细胞系的重视，我们不仅对其精心培养，同时进行了克隆，成功地获得了稳定的不死性的对病毒广谱敏感的克隆株，分别在汕头华茵和北京等三个实验室保存。

CDNA fragment of cadherin-like protein gene in the fifth instar larva of oriental tobacco budworm was amplified using degeneracy primers and RT-PCR technique, and the target fragment was further sequenced after being cloned into pMD18-T vector plasmid.

本研究以烟夜蛾五龄幼虫中肠的总RNA为模板，根据已经克隆的其他昆虫的类钙粘蛋白基因序列设计引物，利用RT-PCR技术扩增出了烟夜蛾类钙粘蛋白基因的cDNA片段，将其连接到pMD18-T载体，转化大肠杆菌后筛选阳性克隆并进行序列测定，测序得到的1335bP的片断编码444个氨基酸残基。

Insect resistance to Bt toxin is related with the variation of cadherin-like protein gene.cDNA fragment of cadherin-like protein gene in the fifth instar larva of oriental tobacco budworm was amplified using degeneracy primers and RT-PCR technique,and the target fragment was further sequenced after being cloned into pMD18-T vector plasmid.

本研究以烟夜蛾五龄幼虫中肠的总RNA为模板，根据已经克隆的其他昆虫的类钙粘蛋白基因序列设计引物，利用RT-PCR技术扩增出了烟夜蛾类钙粘蛋白基因的cDNA片段，将其连接到pMD18-T载体，转化大肠杆菌后筛选阳性克隆并进行序列测定，测序得到的1 335 bp的片断编码444个氨基酸残基。

With primers designed according to sequence(AY052417) of diapause pupae of oriental tobacco budworm registered on GenBank, a double-strand cDNA fragment of DH gene was obtained from RT-PCR amplification and was further cloned into pGEM-T Easy vector.

根据GenBank发表序列(AY052417)设计引物，以烟夜蛾(Helicoverpa assulta Guenée)滞育蛹为材料，利用RT-PCR技术扩增到滞育激素(diapause hormone，DH)cDNA，并通过TA克隆的方法将其克隆至pGEM-T Easy载体上，对目的基因进行测序，并利用设计的酶切位点将目的基因从T载体上切下。

This research is based on our previous successful cloning of a completely novel calcium metabolism related protein—calmodulin-like protein from pearl oyster and calmodulin. The main purpose of this research is to find out the function of CaLP on the cell level and compare functions of these two proteins.

为进一步揭示贝类生物矿化机理，本研究以目前我国用于生产海水珍珠的主要贝类合浦珠母贝为研究材料，以本实验室已克隆出的新蛋白钙调素类似蛋白(calmodulin-like protein，CaLP)为研究对象，对CaLP在细胞水平上的功能进行了进一步的研究，并将其与本实验室已克隆出来的另一钙代谢相关蛋白钙调素(calmodulin，CaM)进行了比较。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。