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The profilin of Caryota mitis pollen was cloned and expressed successfully in E. coli BL21 (DE3), which will be used for further study on Caryota mitis pollen related allergy.

成功地克隆和表达了短穗鱼尾葵花粉profilin,为短穗鱼尾葵花粉过敏的诊断和免疫治疗奠定了基础。

Methods RT-PCR and RACE methods were applied to clone the full-length panallergen gene from Caryota mitis pollen. The sequence of the insert was analyzed.

利用RT-PCR结合RACE技术克隆短穗鱼尾葵花粉中泛变应原profilin的全长基因,并进行序列分析。

The ORF of profilin of Caryota mitis pollen was amplified by RTPCR using specific primers and cloned into the expression vector pET-28a.

结果克隆获得了短穗鱼尾葵花粉profilin的全长基因,由608个碱基组成,开放阅读框为396个碱基,编码131个氨基酸。

IgE reactivity of recombinant Caryota mitis pollen profilin was investigated by western blot. Results The Caryota mitis pollen profilin gene was cloned. The cloned fragment was 608bp in length, with an 396bp open reading frame coding for 131 amino acids.

结果 克隆获得了短穗鱼尾葵花粉profilin的全长基因,由608个碱基组成,开放阅读框为396个碱基,编码131个氨基酸。

Methods Mono- and poly-clonal immunohistochemistry methods were used to compare the positive rate of the expression of Col X in articular cartilage of KBD patients with the control.

用单克隆和多克隆免疫组化法检测大骨节病与对照组关节软骨X型胶原表型和bFGF表达。

Therefore,this is the first receptor-like resistance geneisolated from wheat,a monocotyledon crop.TaLRR1 encode a membrane anchoringglycoprotein that can be divided into seven domains.

这是首次从小麦中克隆到的与双子叶植物番茄的Cf-2和Cf-4有类似结构的细胞外受体类抗病候选基因。

Seagrasses are monocotyledonous species that are adapted to live and reproduce entirely in the marine environments, where they suffer from a variety of disturbances, such as tides, hurricanes. To adapt to these environments, seagrasses have evolved a series of characteristics, among which strong clonality is a prominent one.

海草是适应在海洋环境中生存和繁殖的单子叶植物,由于所处环境常存在潮汐、风暴等的干扰,海草形成了一系列适应特征,克隆性是其中突出的一个。

Synthesis of monoglyceride comtaining omega-3 fatty acids by microbial lipase in organic solvent.

邓子新。类产碱假单胞菌耐热碱性脂肪基因的克隆

It is as if we were watching a new Monroe—or a cloned Monroe.

因为展现在我们面前的就好像是一位新的梦露——或者说是克隆的梦露。

She's just another Marilyn Monroe clone.

她仿佛是从玛丽莲·梦露克隆而来的。

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It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发,分析对称连杆曲线上鲍尔点的产生条件,提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品,年生产总值3000万美元,产品价廉物美、选料上乘、质量保证,深受国内外客户的青睐

The eˉtiology of hemospermia is complicate,but almost of hemospermia are benign.

血精的原因很,以良性病变为主。